pH-dependent Cytotoxicity of a Peptide Toxin, Tolaasin

펩티드 독소 Tolaasin에 의한 세포독성의 pH 의존성

  • Kim, Sung-Tae (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Choi, Tae-Keun (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Kim, Young-Kee (Department of Agricultural Chemistry, Chungbuk National University)
  • 김성태 (충북대학교 농업생명환경대학 농화학과) ;
  • 최태근 (충북대학교 농업생명환경대학 농화학과) ;
  • 김영기 (충북대학교 농업생명환경대학 농화학과)
  • Published : 2007.12.31

Abstract

Tolaasin, a peptide toxin produced by Pseudomonas tolaasii, causes a serious disease on the cultivated mushrooms, known as brown blotch disease. Hemolysis using red blood cells was designed to measure the cytotoxicity of tolaasin molecules. Since tolaasin has two amine groups near the C-terminus, its membrane binding will be dependent on the ionic states of the amine groups. When the tolaasin peptide was titrated, its titration curve indicated the presence of titratable amine(s) at pH ranges from 7.0 to 9.6. When the pH-dependence of tolaasin-induced hemolysis was measured at various pHs, hemolysis was more efficient at alkaline pHs. In order to measure the membrane binding activity of tolaasin at different pHs, RBCs were incubated with tolaasin molecules for short time periods and washed out with fresh buffer. Because of the tolaasin binding during the preincubation period, fast hemolyses were observed at pH 8 or higher. These results imply that non-charged or less positively charged states of tolaasin molecules easily bind to membrane and show high hemolytic activity.

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