Screening of Antimicrobial Lactic Acid Bacteria against Bovine Mastitis

여러 분리원으로부터 유방염 원인균에 대한 항균력을 가진 유산균의 분리

  • Lee, Na-Kyoung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Choi, In-Ae (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Park, Yong-Ho (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Jong-Man (National Veterinary Research & Quarantine Service) ;
  • Kim, Jae-Myung (National Veterinary Research & Quarantine Service) ;
  • Jung, Suk-Chan (National Veterinary Research & Quarantine Service) ;
  • Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • 이나경 (건국대학교 축산식품생물공학과) ;
  • 최인애 (건국대학교 축산식품생물공학과) ;
  • 박용호 (서울대학교 수의과대학) ;
  • 김종만 (국립수의과학검역원) ;
  • 김재명 (국립수의과학검역원) ;
  • 정석찬 (국립수의과학검역원) ;
  • 백현동 (건국대학교 축산식품생물공학과)
  • Published : 2007.12.30


Bovine mastitis is costly infectious disease of dairy cattle, being responsible for significant economic losses all over the world. Also, mastitis has troubled about resistance to antibiotics. The purpose of this study was to screen a novel antimicrobial strain from various sources (raw milk and feeds (from farm of Paju, Dangjin, and Hwasung), commercial milk, Korean traditional fermented foods, and chicken feces). The isolate was screened using triple agar layer method and deferred method was used for confirmation of antimicrobial effect. Seventy six of isolates were screened using triple agar layer method. In these strain, 42 isolates were shown a broad spectrum of autimicrobial activity against mastitis pathogens. Especially, fourteen isolates were shown over 20 mm inhibition zone against S. aureous ATCC 25923. These results suggest that these novel antimicrobial strains could be used for the alternative of antibiotics.


  1. Bongaerts, G. P. A. and Severijnen, R. S. V. M. (2001) The beneficial, antimicrobial effect of probiotics. Med. Hypotheses 56, 174-177
  2. Britten, A. M. (1998) Delivering mastitis control systems to your clients. Large Anim. Pract. 19, 14-21
  3. Jain, N. C. (1979) Common mammary pathogens and factors in infection and mastitis. J. Dairy Sci. 62, 128-134
  4. Jo, S. N., Liu, J., Lee, S. E., Hong, M. S., Kim, D. H., Kim, M. C., Cho, S. W., and Jun, M. H. (2005) A therapeutic effect of ozonated oil on bovine mastitis. J. Vet. Clin. 22, 318-321
  5. Kang, H. M., Moon, J. S., Jang, G. C., Kim, J. M., Song, M. D., and Yang, S. Y. (2005) Antibacterial effects of Terminaliae chebula extract against major pathogens and methicillinresistant Staphylococcus aureus (MRSA) from bovine mastitis milk. Korean J. Vet. Res. 45, 113-119
  6. Kwon, H. J., Liu, J., Jo, S. N., Song, K. H., Kim, D. H., Jun, M. H., Cho, S. W., Kim, M. C., and Yoon, H. I. (2005) Therapeutic effect of ozone gas on bovine mastitis. J. Vet. Clin. 22, 314-317
  7. Lee, E. S., Kang, H. M., Chung, C. I., and Moon, J. S. (2007A) Antimicrobial susceptibility and prevalence of gram-negative bacteria isolated from bovine mastitis. Korean J. Vet. Res. 47, 67-75
  8. McDonald, J. S., McDonald, T. J., and Anderson, A. J. (1977) Antimicrobial sensitivity of aerobic gram-negative rods isolated from bovine udder infections. Am. J. Vet. Res. 38, 1503-1507
  9. Palombo, E. A. and Semple, S. J. (2002) Antibacterial activity of Australian plant extracts against methicillin-resistant Staphylococcus au reus (MRSA) and vancomycin-resistant enterococci (VRE). J. Basic Microb. 42, 444-448<444::AID-JOBM444>3.0.CO;2-8
  10. Watts, J. L. (1988) Etiological agents of bovine mastitis. Vet. Microbiol. 16, 41-66
  11. O'Sullivan, L., Ryan, M. P., Ross, R. P., and Hill, C. (2003) Generation of food-grade lactococcal starters which produce the lantibiotics lacticin 3147 and lacticin 481. Appl. Environ. Microbiol. 69, 3681-3685
  12. Lee, G., Kang, H. M., Chung C. I., and Moon, J. S. (2007B) Antimicrobial susceptibility and genetic characteristics of Streptococcus uberis isolated from bovine mastitis milk. Korean J. Vet. Res. 47, 33-41
  13. McDonald, J. S. (1975) Effect of milking machine design and function on new intramammry infection. J. Milk Food Technol. 38, 44-51
  14. Nam, H. M., Moon, J. S., Joo, Y. S., Park, Y. H., and Han, H. R. (2006) Effects of injectable B-carotene on mastitis in dairy cows. Korean J. Vet. Res. 46, 149-158
  15. Smith, K. L. and Hogan, J. S. (1993) Environmental mastitis. Vet. Clin. N. Am.-Food A. 9, 489-498
  16. Kim, H. J., Kim, J. H., Son, J. H., Sea, H. J., Park, S. J., Park, N. S., and Kim, S. K. (2004) Characterization of bacteriocin produced by Lactobacillus bulgaricus. J. Microbiol. Biotechnol. 14, 503-508
  17. Bradley, A. (2002) Bovine mastitis: an evolving disease. Vet. J. 164, 116-128