Inhibitory Effect on Rotavirus by Exopolysaccharides Extracted from Kefir

Kefir에서 추출한 Exopolysaccharide의 Rotavirus의 저해효과

  • Song, Jin-Ook (Department of Science and Technology Education, Cyber Graduate School, Joongbu University) ;
  • Kim, Tae-Jin (R&D Center, Maeil Dairy Industry CO., LTD.) ;
  • Kim, Yong-Hui (Department of Hotel and Foodservice Industry, Joongbu University)
  • 송진욱 (중부대학교 원격대학원 과학기술교육학과) ;
  • 김태진 (매일유업(주) 중앙연구소) ;
  • 김용휘 (중부대학교 호텔외식산업학과)
  • Published : 2007.12.30


This study was carried out to investigate the toxicity of exopolysaccharides (EPS) from kefir toward MA104 cells and evaluate the inhibitory effects of kefir EPS on rotavirus infection. The results obtained are summarized as follows: Lactic acid bacteria (Lactobacillus fermentum, L. acidophilus, L. brevis) and yeasts (Candida kefyr, Cryptococcus albidus, Pichia ohmeri) were isolated and identified from kefir grain and culture. At 1% EPS, the inhibitory effects of EPS on the infection of MA-104 cells using the MTT assay were $72.52{\pm}6.48%$ for human rotavirus (KU), $36.06{\pm}7.63%$ for bovine rotavirus (NCDV), and $81.66{\pm}1.11%$ for porcine rotavirus (OSU). At 1/128% EPS, the effects were $24.98{\pm}4.58%$ for human rotavirus (KU), $4.71{\pm}6.16%$ for bovine rotavirus (NCDV), and $4.05{\pm}14.90%$ forporcine rotavirus (OSU). EPS isolated from kefir have inhibitory effects on rotaviruses of various serotypes and rotaviruses from different animals.


kefir;exopolysaccharide (EPS);rotavirus;MTT assay


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