The Antimutagenic and Antioxidant Effects of Red Pepper Seed and Red Pepper Pericarp (Capsicum annuum L.)

  • Sim, Ki-Hyeon (Department of Food & Nutrition, Sookmyung Women's University) ;
  • Han, Young-Sil (Department of Food & Nutrition, Sookmyung Women's University)
  • Published : 2007.12.31


In this study, we examined the antimutagenicity of red pepper seed and red pepper pericarp ethanol extracts using the standard Ames test in the presence and absence of S9 mix. The extracts showed inhibitory effects on both the TA98 and TA100 Salmenella Typhimurium strains against the mutagenic activity of promutagen 2-aminoanthracene, and were also protective against the directly acting mutagens sodium azide and 2-nitrofluorene. The red pepper seed elicited stronger antimutagenicity than the red pepper pericarp. Both the red pepper seed and red pepper pericarp directly quenched nitric oxide to different degrees and the scavenging activities increased with increasing concentrations. Nitric oxide scavenging activity ranged from $22{\sim}77%$ in the red pepper seed, and from $36{\sim}49%$ in the red pepper pericarp. The TEAC values for red pepper seed extract were $47.89{\pm}1.64mg\;g^{-1}$ in the ABTS radical scavenging assays, while those of red pepper pericarp extract were $94.18{\pm}1.61mg\;g^{-1}$. Therefore, we conclude that red pepper seed and red pepper pericarp have antimutagenic activities as well as antioxidant activity.


  1. Matsufuji H, Nakamuro H, Chino M, Mitsuharo T. 1998. Antioxidant activity of capsanthin and the fatty acid esters in paprika (Capsicum annuum). J Agric Food Chem 46: 3462-3472
  2. Hornero-Mendez D, Guevara RGL, Mínguez-Mosquera MI. 2000. Carotenoid Biosynthesis Changes in five red pepper (Capsicum annuum L.) cultivars during ripening. Cultivar selection for breeding. J Agric Food Chem 48: 3857-3864
  3. Hasler CM. 1998. Functional foods: their role in disease prevention and health. Food Technol 52: 63-69
  4. Ou B, Huang D, Hampschwoodwill M, Flanagan TA, Deemer EK. 2002. Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity and FRAP assays, a comparative study. J Agric Food Chem 50: 3122-3128
  5. Morimoto C, Tsujita T, Okuda H. 1997. Epinephrine- induced lipolysis in rats fat cell from visceral and subcutaneous sites: role of hormone-sensitive and lipid droplets. J Lipid Res 38: 132-138
  6. Park JS, Park KY, Yu R. 1998. Inhibition of nitrosation by capsaicin and its metabolism. J Korean Soc Food Sci Nutr 27: 1015-1018
  7. Choi SM, Jeon YS, Park KY, Jung KO. 2001. Antimutagenic effects of different kinds and parts of red pepper powder on the N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutagenicities. J Korean Cancer Prev 6: 108-115
  8. Yang KS, Yu JH, Hwang JI, Yang R. 1974. Synergistic effect of citric acid on antioxidant property of red pepper. Korean J Food Sci Technol 6: 193-198
  9. Shalini VK, Srinivas L. 1987. Lipid peroxide induced DNA damage: protection by turmeric (C. longa). Mol Cell Biochem 77: 39-48
  10. Srinivasan K. 2005. Spices as influencers of body metabolism: an overview of three decade of research. Food Res Int 38: 77-86
  11. Shalini VK, Srinivas L. 1990. Fuel smoke condensate induced DNA damage in human lymphocytes and protection by turmeric. Mol Cell Biochem 95: 21-30
  12. Kim SH, Park KY, Sur MJ. 1991. Inhibitory effect of aflatoxin B1 mediated mutagenicity by red pepper powder in the Salmonella assay system. J Korean Soc Food Nutr 20: 156-161
  13. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173-215
  14. Kato H, Lee IE, Chyen N, Kim SB, Hayase F. 1987. Uninhibitory of nitrosamine formation by nondialyzable melanoidins. Agric Bio Chem 51: 1333-1338
  15. Manjeshwar SB, Ganesh CJ, Shaival KR, Kiran BS. 2003. Evaluation of nitric oxide scavenging activity of certain spices in vitro: A preliminary study. Nahrung/Food 47: 261-264
  16. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237
  17. Choi SM, Jeon YS, Jung KO, Park KY. 2002. Fermentation characteristics and antimutagenicity of kimchi that prepared with different ratio of seed in red pepper powder. J Korean Cancer Prev 7: 51-59
  18. Surh YJ, Lee RCJ, Park KK, Mayne ST, Kiem A, Miller JA. 1995. Chemoprotective effects of capsaicin and diallyl sulfide against mutagenesis or tumorigenesis by vinyl carbamate and N-nitrosodimethylamine. Carcinogenesis 16: 2467-2471
  19. Kumaran A, Karunakaran RJ. 2007. In viro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT 40: 344-352
  20. Katalinic V, Milos M, Kulisic T, Jukic M. 2006 Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 94: 550-557
  21. Theriault M, Caillet S, Kermasha S, Lacroix M. 2006. Antioxidant, antiradical and antimutagenic activities of phenolic compounds present in maple products. Food Chem 98: 490-501
  22. Sharififar F, Moshafi MH, Mansouri SH, Khodashenas M, Khoshnoodi M. 2007. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control 18: 800-805
  23. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84: 407-412
  24. Rice-Evans C, Miller NJ. 1994. Total antioxidant status in plasma and body fluids. Meth Enzym 234: 279-293
  25. Kim SN, Lee KW, Park JB, Lee HJ, Hwang IK. 2006. Effect of drying in antioxidant activity and changes of ascorbic acid and colour by different drying and storage in Korean red pepper (Capsicum annuum L.). Int J Food Sci Technol 41: 90-95
  26. Navarro J, Flores P, Garrido C, Martinez V. 2006. Changes in the contents of antioxidant compounds in pepper fruits at different ripening stages, as affected by salinity. Food Chem 96: 66-73
  27. Rice-Evans C, Miller NJ, Paganda G. 1996. Structure- antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 20: 933-956
  28. Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW. 1998. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agr Food Chem 46: 1887-1892
  29. Riedl K, Hagerman AE. 2001. Tannin-protein complexes as radical scavengers and radical sinks. J Agr Food Chem 49: 4917-4923
  30. Newman MJ. 1990. Inhibition of carcinoma and melanoma cell growth by type I transforming growth factor $\beta$1 is dependent on the presence of poly-unsaturated fatty acids. Pro NAtl Acad Sci 87: 5543-5547
  31. Kwon YM, Rhee SH, Park KY. 1995. Antimutagenin effects of juice from peppers in Salmonella assay system. J Korean Soc Food Nutr 24: 162-163

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