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Antioxidant Properties of Ginseng (P. ginseng C.A. Meyer) Extracts by Organic Solvent Fractionation

  • Kim, Ji-Sang ;
  • Moon, Gap-Soon ;
  • Kim, Hyun-Oh ;
  • Lee, Young-Soon
  • Published : 2007.12.31

Abstract

The objective of this study was to investigate antioxidant activities of Panax ginseng extracted with various solvents including n-hexane, chloroform, EtOAC, n-butanol and water. Among the various ginseng extracts, ethyl acetate (EtOAC) extracts showed the most powerful scavenging activities against DPPH radicals. Among the other solvent extracts, the butanol extract seemed relatively more effective in scavenging activity, followed by chloroform, water and hexane extracts. Moreover, the highest reducing power and ferrous ion chelating activity were found in the EtOAC extract followed by other extracts of ginseng. EtOAC extracts, which exhibited the best antioxidant activities of all solvent extracts of ginseng, possessed higher concentrations of total phenolics (777.61 mg/100 g) than other extracts. These results suggest that EtOAC extracts of ginseng (P. ginseng C.A. Meyer) have the most effective antioxidant capacity compared to n-hexane, chloroform, n-butanol and water tested in this study, and has important applications for the pharmaceutical and food industries.

Keywords

antioxidant properties;ginseng;organic solvent fractionation;polarity

References

  1. Sugaya A, Yuzurihara M, Tsuda T, Yasuda K, Kajiwara K, Sugaya E. 1998. Proliferative effect of ginseng saponin on neurite extension of primary cultured neurons of the rat cerebral cortex. J Ethnopharmacol 22: 173-181 https://doi.org/10.1016/0378-8741(88)90125-0
  2. Banerjce U, Izquierdo JA. 1982. Antistress and antifatigue properties of Panax ginseng: Comparison with piracetan. Acta Physiol Lat Am 32: 277-285
  3. Deng HW, Guan YY, Kwan CY. 1990. Effects of ginseng saponins on lipid peroxidation in liver and cardiac muscle homogenates. Biochem Arch 6: 359-365
  4. Sohn HO, Lim HB, Lee YG, Lee DW, Kim YT. 1993. Effect of subchronic administration of antioxidants against cigarette smoke exposure in rats. Arch Toxicol 67: 667- 673 https://doi.org/10.1007/BF01973689
  5. Attele AS, Wu JA, Yuan CS. 1999. Ginseng pharmacology-multiple constituents and multiple actions. Biochem Pharmacol 58: 1685-1693 https://doi.org/10.1016/S0006-2952(99)00212-9
  6. Ren G, Chen F. 1999. Simultaneous quantification of ginsenosides in American ginseng (Panax quinquefolium) root powder by visible/near-infrared reflectance spectroscopy. J Agric Food Chem 47: 2771-2775 https://doi.org/10.1021/jf9812477
  7. Ali MB, Hahn EJ, Paek KY. 2005. CO2-induced total phenolics in suspension cultures of Panax ginseng C.A. Mayer roots: role of antioxidants and enzymes. Plant Physiol Biochem 43: 449-457 https://doi.org/10.1016/j.plaphy.2005.03.005
  8. Zheng W, Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 49: 5165-5170 https://doi.org/10.1021/jf010697n
  9. Wanasundara PKJPD, Shahidi F. 1996. Optimization of hexametaphosphate-assisted extraction of flaxseed proteins using response surface methodology. J Food Sci 61: 604-607 https://doi.org/10.1111/j.1365-2621.1996.tb13168.x
  10. Zhang D, Yasuda T, Yu Y. 1996. Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by iron mediated lipid peroxidation. Free Radic Biol Med 20: 145-150 https://doi.org/10.1016/0891-5849(95)02020-9
  11. Leanderson P, Faresjo AO, Tagesson C. 1997. Green tea polyphenols inhibit oxidant-induced DNA strand breakage in cultured lung cells. Free Radic Biol Med 23: 235-242 https://doi.org/10.1016/S0891-5849(96)00590-4
  12. Goldbohm RA, Hertog MG, Brants HA, van Poppel G, van den Brandt PA. 1996. Consumption of black tea and cancer risk: a prospective cohort study. J Natl Cancer Inst 88: 93-110 https://doi.org/10.1093/jnci/88.2.93
  13. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu's reagent. Meth Enzymol 299: 152-178 https://doi.org/10.1016/S0076-6879(99)99017-1
  14. Yen GC, Hsieh PP. 1995. Antioxidative activity and scavenging effects on xylose-lysine Maillard reaction products. J Sci Food Agric 67: 415-420 https://doi.org/10.1002/jsfa.2740670320
  15. Singh N, Rajini PS. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85: 611-616 https://doi.org/10.1016/j.foodchem.2003.07.003
  16. Dinis TCP, Madeira VMC, Almerida LM. 1994. Action of phenolic derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavenges. Arch Biochem Biophys 315: 161-16 https://doi.org/10.1006/abbi.1994.1485
  17. Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44: 307- 315 https://doi.org/10.5264/eiyogakuzashi.44.307
  18. Kumaran A, Joel Karunakaran R. 2007. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Lebensmittel-Wissenschaft und -Technologie 40: 344-352 https://doi.org/10.1016/j.lwt.2005.09.011
  19. Lee JC, Lim KT, Jang YS. 2002. Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim Biophys Acta 1570: 181-191 https://doi.org/10.1016/S0304-4165(02)00196-4
  20. Yen GC, Hsieh CL. 1998. Antioxidant activity of extracts from Du-zhong (Eucommia ulmoides) toward various lipid peroxidation models in vitro. J Agric Food Chem 46: 3952-3957 https://doi.org/10.1021/jf9800458
  21. Osawa T. 1994. Novel natural antioxidants for utilization in food and biological systems. In Postharvest Biochemistry of Plant Food-Materials in the Tropics. Uritani I, Garcia VV, Mendoza EM, eds. Japan Scientific Societies Press, Japan. p 241-251
  22. Ozcelik B, Lee JH, Min DB. 2003. Effect of light, oxygen, and pH on the absorbance of 2,2-diphenyl-1-picrylhydrazyl. J Food Sci 68: 487-490 https://doi.org/10.1111/j.1365-2621.2003.tb05699.x
  23. Matthaus B. 2002. Antioxidant activity of extracts obtained from residues of different oilseeds. J Agric Food Chem 50: 3444-3452 https://doi.org/10.1021/jf011440s
  24. Shih PW, Lai PL, Jen HWK. 2006. Antioxidant activities of aqueous extracts of selected plants. Food Chem 99: 775-783 https://doi.org/10.1016/j.foodchem.2005.07.058
  25. Robards K, Prenzeler PD, Tucker G, Swatsitang P, Glover W. 1999. Phenolic compounds and their role in oxidative process in fruits. Food Chem 66: 401-436 https://doi.org/10.1016/S0308-8146(99)00093-X
  26. Pyo YH, Lee TC, Logendra L, Rosen RT. 2004. Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chem 85: 19-26 https://doi.org/10.1016/S0308-8146(03)00294-2
  27. Rice-Evans CA, Miller NJ, Paganga G. 1996. Structure- antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 20: 933-956 https://doi.org/10.1016/0891-5849(95)02227-9
  28. Ramarathnam N, Ochi H, Takeuchi M. 1997. Antioxidant defense system in vegetable extracts. In Natural Antioxidants: Chemistry, Health Effects and Applications. Shahidi F, ed. AOCS Press, IL, USA. p 76-87
  29. Frankel EN. 1991. Recent advances in lipid oxidation. J Agric Food Chem 54: 495-511 https://doi.org/10.1002/jsfa.2740540402
  30. Haber F, Weiss J. 1934. The catalytic decomposition of hydrogen peroxide by iron salts. Proc Royal Soc London Serv A 147: 332-351
  31. Miller DD. 1996. Mineral. In Food Chemistry. Marcel D, ed. Marcel Dekker, New York, USA. p 618-649
  32. Halliwell B, Gutteridge JMC. 1984. Oxygen toxicology, oxygen radicals, transition metals and disease. Biochem J 219: 1-4 https://doi.org/10.1042/bj2190001
  33. Chandrika M, Liyana P, Fereidoon S. 2007. Antioxidant and free radical scavenging activities of whole wheat and milling fractions. Food Chem 101: 1151-1157 https://doi.org/10.1016/j.foodchem.2006.03.016
  34. Pazos M, Gallardo JM, Torres JL, Medina I. 2005. Activity of grape polyphenols as inhibitors of the oxidation of fish lipids and fish muscle. Food Chem 92: 547-557 https://doi.org/10.1016/j.foodchem.2004.07.036
  35. Yamaguchi R, Tatsumi MA, Kato K, Yoshimitsu U. 1988. Effect of metal salts and fructose on the autoxidation of methyl linoleate in emulsions. Agric Biol Chem 52: 849-850 https://doi.org/10.1271/bbb1961.52.849
  36. Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. 2002. Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. J Agric Food Chem 50: 2454-2458 https://doi.org/10.1021/jf011369q
  37. Duh PD. 1998. Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free radical and active oxygen. J Am Oil Chem Soc 75: 455-461 https://doi.org/10.1007/s11746-998-0248-8
  38. Tanaka M, Kuie CW, Nagashima Y, Taguchi T. 1988. Applications of antioxidative Maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakk 54: 1409-1414 https://doi.org/10.2331/suisan.54.1409
  39. Shon MY, Kim TH, Sung NJ. 2003. Antioxidants and free radical scavenging activity of Phellinus baumii extracts. Food Chem 82: 593-59 https://doi.org/10.1016/S0308-8146(03)00015-3
  40. Xing R, Liu S, Guo Z. 2006. The antioxidant activity of glucosamine hydrochloride in vitro. Bioorg Med Chem 14: 1706-1709 https://doi.org/10.1016/j.bmc.2005.10.018
  41. Zhao GR, Xiang ZJ, Ye TX, Yuan YJ, Guo ZX. 2006. Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chem 99: 767-774 https://doi.org/10.1016/j.foodchem.2005.09.002

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