Enhancement of Bacteriocin Production by Bacillus subtilis cx1 in the Presence of Bacillus subtilis ATCC6633

Bacillus subtilis ATCC6633이 Bacillus subtilis cx1의 박테리오신 생산에 미치는 유도효과

  • Chang Mi (Department of Food and Nutrition, Chosun University) ;
  • Chang Hae-Choon (Department of Food and Nutrition, Chosun University)
  • 장미 (조선대학교 식품영양학과) ;
  • 장해춘 (조선대학교 식품영양학과)
  • Published : 2006.09.01


BSCX1 was an antimicrobial peptide produced by Bacillus subtilis cx1. Attempts were made to determine the location of inducing factor in the bacteriocin-sensitive cell affecting bacteriocin BSCX1 production. Mixed culture of the bacteriocin producer strain B. subtilis cx1 and its sensitive strain B. subtilis ATCC6633, increased production of bacteriocin BSCX1. The result suggested the presence of a bacteriocin inducing factor in the sensitive strain. The inducing factor was localized in the cell debris and intracellular fraction of B. subtilis ATCC6633. Bacteriocin BSCX1 inducing factor was found to be highly stable in the pH range 2.5-9.5, but inactivated within 3h over $50^{\circ}C$, and treatment with proteinase K destroyed its inducing activity, this result suggested that the inducing factor should be a proteinaceous nature.


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