Simultaneous detection for synthetic antimicrobials in muscle by high performance liquid chromatography-mass selective detector (HPLC-MSD)

HPLC-MSD 를 이용한 식육 중 합성항균제의 동시분석

  • Hong In-Suk (Seoul Metropolitan Health & Environment Research Institute) ;
  • Choi Yoon-Hwa (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kwon Taek-Boo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Lee Jung-Hark (Seoul Metropolitan Health & Environment Research Institute)
  • 홍인석 (서울특별시 보건환경연구원) ;
  • 최윤화 (서울특별시 보건환경연구원) ;
  • 권택부 (서울특별시 보건환경연구원) ;
  • 이정학 (서울특별시 보건환경연구원)
  • Published : 2006.09.01

Abstract

This study was conducted to develop the analytical method about simultaneous determination for synthetic antimicrobials in muscle by high performance liquid chromatography - mass selective detector (HPLC- MSD). Solid phase extraction (SPE), matrix solid phase dispersion (MSPD) and liquid-liquid extraction (LLE) have been adapted as pretreatment procedures for HPLC- MSD. Among various solvent tested, methanol was chosen for extraction of synthetic antimicrobials in muscles. For the optimized response, the values of various MS parameters including fragment voltage, drying gas flow, nebulizer pressure, drying gas temperature were verified. The average recovery rates using MSPD and SPE for muscles of bovine and pork were 78.9-127.1% and 78.3-121.7%, respectively. This method was verified the satisfactory performance for fourteen synthetic antimicrobials excepting carbadox in muscle of pork as detection limit of $0.05{\mu}g/g$ on API/ES SIM mode.

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