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Development of a Fiber-Optic Biosensor for the Detection of Listeria monocytogenes

리스테리아 식중독균 검출을 위한 광학식 바이오센서 개발

  • Kim G. (National Institute of Agricultural Engineering, RDA) ;
  • Choi K.H. (National Institute of Agricultural Engineering, RDA)
  • Published : 2006.04.01

Abstract

Frequent outbreaks of foodborne illness demand the need for rapid and sensitive methods for detection of these pathogens. Recent development of biosensor technology has a great potential to meet the need for rapid and sensitive pathogens detection from foods. An antibody-based fiber-optic biosensor and an automated reagents supply system to detect Listeria monocytogenes were developed. The biosensor for detection of Listeria monocytogenes in PBS and bacteria spiked food samples was evaluated. The automated reagents supply system eliminated cumbersome sample and detection antibody injection procedures that had been done manually. The biosensor could detect $10^4$ cfu/ml of Listeria monocytogenes in PBS. By using the fiber-optic biosensor, $2x10^8$ cfu/ml of Listeria monocytogenes in the food samples were detectable.

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