Effective in Vitro Propagation by Bulb Scale Segments Culture of Muscari comosum var. plumosum

  • Ko Jeong-Ae (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Choi Jeong-Ran (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Xudong He (The Key Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University) ;
  • Kim Hyun-Soon (International Technical Cooperation Center Rural Development Administration)
  • Published : 2006.06.01

Abstract

A rapid and mass propagation method for multiple shoots and plant regeneration using bulb scales of Muscari comosum var. plumosum were developed. In vitro different parts of bulb scale as explants were cultured on 11 kinds of MS (1962) media supplemented with various plant growth regulators to induce shoot and callus. A combination of 2.0 mg/L 6-BA and 2.0 mg/L IBA on MS medium was the most favorable and induced the highest production (80%) of shoot formation after 30 days. We also found that the middle part of bulb scale was the best for mass propagation of Muscari comosum var. plumosum of which production could reach 64.4%.

References

  1. Bae, H.C., H.G. Ahn, S.K. Park, W.M. Yue, W.Y. Jung, Y.D. Chang and S.T. Choi. 2000. Effect of scaling time and scale position on bulblet formation in scaling of Muscari armeniacum 'early giant'. J. Kor. Soc. Hort. Sci. 41: 93-95
  2. Choi, S.T., S.K. Park, W.Y. Jung, H.G. Ahn, I.H. Park, Y.D. Chang and S.T. Kim. 2000. Effect of leaf cutting time and leaf part on bulblet formation ofMuscari armeniacum 'early gaint'. Soc. Horc. Sci. 41: 87-89
  3. Godo, T., K. Kobayashi, T. Tagami, K. Matsui and T. Kida. 1998. In vitro propagation utilizing suspension cultures ofmeristemic nodular cell clumps and chromosome stability of Lilium formolongi hort. Sci. Hort. 72: 193202
  4. Hou, Q., J.G. Carman and W.A. Varga. 1997. Micropropagation of Sego lily. Plant Cell Tissue and Organ Culture 49: 149-151 https://doi.org/10.1023/A:1005883528776
  5. Li, Q.X., Q.F. Zhao and H.L. Li. 2004, Study on tissue culture and propagation of Nettuno, Journal of Northwest Normal University (Natural Science) 40: 18-20
  6. Maesato, K., K. Sharada, H. Fukui, T. Hara and K.S. Sarma. 1994. In vitro bulblet regulation from bulbscale explants of Lilium japonicum Thunb.: effect of plant growth regulators and culture environment. J. Hort. Sci. 69: 289297
  7. Masaru, N., S. Tanaka, S. Kagami and H. Saito. 2005. Plantlet regeneration from protoplasts of Muscari armeniacum leichtl.ex bak. Plant Biotechnology 22: 249-251 https://doi.org/10.5511/plantbiotechnology.22.249
  8. Park, S.K., H.G. Ahn, W.Y. Jung, Y.D. Chang, S.T.Kim, I.H. Park and S.T. Choi. 2000. Effect of leaf order and position on bulblet formation in leaf cutting of Muscari armeniacum 'early giant'. Soc. Hort. Sci. 41: 90-92
  9. Peck, D.E. and B.G. Cumming. 1986. Beneficial effects of activated charcoal on bulblet production in tissue cultures of Muscari armeniacum. Plant Cell Tissue Organ Culture 6: 9-14 https://doi.org/10.1007/BF00037753
  10. Pierik, R.L.M. and A.J.M. Post. 1975. Rapid vegetative propagation of Hyacinthus orientalis L. in vitro. ScientiaHort. 3: 293-297 https://doi.org/10.1016/0304-4238(75)90013-8
  11. Takayama, S. and M.Misawa. 1983. Ascheme for mass propagation of Lilium in vitro. Scientia Horticulturae 18: 353-362 https://doi.org/10.1016/0304-4238(83)90017-1
  12. Wang, F.L., H.G. Zhou, Z.F. Hang and Y.P. Lin. 2005. Study on techniques of rapid propagation by tissue culture of the Asiatic Hybrids, Journal of Anhui Agri. Sci. 33: 820-821
  13. Wang, G.P., Y. Chen, J. Zhou, L. Zhang and M.R. Huang. 2005. Blub induction and plant regeneration of Lycoris longituba. Plant Physiology Communication 41: 457-460
  14. Zhu, J., Q. Zhuge, S.S. Yu and N.J. Chen. 2002, Study on tissue culture propagation technique of Lycoris radiate, Jour. of Zhejiang For. Sci & Tech. 22: 45-48