Effective in Vitro Propagation by Bulb Scale Segments Culture of Muscari comosum var. plumosum

  • Ko Jeong-Ae (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Choi Jeong-Ran (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Xudong He (The Key Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University) ;
  • Kim Hyun-Soon (International Technical Cooperation Center Rural Development Administration)
  • Published : 2006.06.01


A rapid and mass propagation method for multiple shoots and plant regeneration using bulb scales of Muscari comosum var. plumosum were developed. In vitro different parts of bulb scale as explants were cultured on 11 kinds of MS (1962) media supplemented with various plant growth regulators to induce shoot and callus. A combination of 2.0 mg/L 6-BA and 2.0 mg/L IBA on MS medium was the most favorable and induced the highest production (80%) of shoot formation after 30 days. We also found that the middle part of bulb scale was the best for mass propagation of Muscari comosum var. plumosum of which production could reach 64.4%.


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