Effects of Sediment Harvesting on Bacterial Community Structure

골재채취가 세균군집구조에 미치는 영향

  • Park, Ji-Eun (Department of Life Sciences, Daegu University) ;
  • Lee, Young-Ok (Department of Life Sciences, Daegu University)
  • 박지은 (대구대학교 자연과학대학 생명과학과) ;
  • 이영옥 (대구대학교 자연과학대학 생명과학과)
  • Published : 2006.06.01

Abstract

The dynamics of bacterial populations belonging to $\alpha\;\beta\;\gamma-subclass$ proteobacteria, Cytophaga-Flavobacterium (CF) group and sulfate reducing bacteria (SRB) in water column of the middle reaches of Nakdong River depending on sediment harvesting were analyzed by fluorescent in situ hybridization (FISH) at sediment harvesting site (near the Seongju bridge) and non-sediment harvesting site (near the Gumi bridge). In addition, some physico-chemical parameters such as temperature, pH, $chi-\alpha$ and electrical conductivity were measured. Regarding the number of total cell counts, cells stained by DAPI, there were no substantial quantitative differences between both sites, but those fluctuation at sediment Harvesting site was greater. And also the ratios of CFgroup and SRB to total cell counts tend to increase at sediment harvesting site with higher $chl-\alpha$, maybe due to the resuspension of sediment into water column. But the total proportion of all determined bacterial populations to total cell counts were greater at non-sediment harvesting site, compared with those at sediment harvesting site. Since the detectibility of bacteria by FISH depends on their metabolic activity, those lower proportion at the sediment harvesting site implies that sediment harvesting may lead to malfunction of those bacteria respect to nutrient recycling and subsequently negative effects on microbial food web.

References

  1. 박정원, 최재신, 김미경. 2004. 낙동강 중류 지역의 부착규조 군집의 변화와 유기오탁지수 (DAIpo)에 의한 수질평가. 한국육수학회지. 37:70-77
  2. Kim HW, KH Chang and GJ Joo. 2005. Characteristics and inter-annual variability of zooplankton dynamics in the middle part of the river (Nakdong River). Korean J. Limnol. 38:412-419
  3. Llobet-Brossa E, R Rosse116-Mora and R Amann. 1998. Microbial community composition of wadden sea sediments as revealed by fluorescence in situ hybridization. Appl. Environ. Microbiol. 64:2691-2696
  4. Schallenberg M and CW Burns. 2004. Effects of sediment resuspension on phytoplankton production:teasing apart the influences of light, nutrients and algal entrainment. Freshwat. Biol. 49:143-159 https://doi.org/10.1046/j.1365-2426.2003.01172.x
  5. Wagner M, GRath, HP Koops, J Floos and R Amann. 1996. In situ analysis of nitrifying bacteria in sewage treatment plants. Wat. Sci. Tech. 34:237-244
  6. APHA, AWWA, WEF. 1995. Standard methods for the examination of water and wastewater. 19th ed. American Public Health Association, Washington, DC
  7. 강영훈, 서준원, 금지돈, 양홍준. 2004. 낙동강 중류 (구미 지역)의 어류군집구조. 한국육수학회지. 37:227-235
  8. Hicks R, R Amann and DA Stahl. 1992 Dual staining of natural bacterioplankton with 4, 6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom level 16S rRNA sequences. Appl. Environ. Microbiol. 58:2158-2136
  9. Manz W, M Wagner, R Amann and KH Schleifer. 1994. In situ characterization of the microbial consortia active in two wastewater treatment plants. Wat. Res. 28: 1715-1723 https://doi.org/10.1016/0043-1354(94)90243-7
  10. 한국건설연구원. 2001. 한국건설연구원 자료집
  11. Manz W, R Amann, W Ludwig, M Wagner and KH Schleifer. 1992. Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions, system. Appl. Microbiol. 15:593-600 https://doi.org/10.1016/S0723-2020(11)80121-9
  12. Ramsing NB, H Fossing, TG Ferdelman. 1996. Distribution of bacterial populations in a stratified Fjord (Mariager Fjord, Denmark) quantified by in situ hydridization and related to chemical gradients in the water column. Appl. Environ. Microbiol. 62:1391-1404
  13. Amann R, W Ludwig and KH Schleifer. 1995. Phylogenetic and in situ detection of individual microbial cells without cultivation. Microbial. Rev. 59: 143-169
  14. Pusceddu A, C Fiordelmondo and R Danovaro. 2005. Sediment resuspension effects on the benthic microbial loop in experimental microcosms. Microbiol. Ecol. 50:602-613 https://doi.org/10.1007/s00248-005-5051-6
  15. Widdel F and Bak F. 1992. Gram-negative mesophilic sulfatereducing bacteria, 3352-3378. In: Balows A, HG Trupper, M Dworkin. W Harder and KH Schleifer. (ed.), The Prokaryotes, vol. 4. Springer-Verlag
  16. DeLong EF, GS Wickham and AA Davis, 1993. Phylogenetic diversity of substrate marine microbial communities from the Atlantics and Pacific Oceans. Science 243:1360-1363 https://doi.org/10.1126/science.2466341
  17. Manz W, U Szewzyk, P Ericsson, R Amann, KH Schleifer and T Stenstom. 1993. In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S rRNA-directed fluorescent oligonucleotide probes. Appl. Environ. Microbiol. 59:2293-2298
  18. Amann R, W Ludwig and KH Schleifer. 1994. Identification of uncultured bacteria: A challenging task for molecular taxonomists. ASM News 60:360-365
  19. Glockner FO, BM Fuchs and R Amann. 1999. Bacterioplankton composition of lake and oceans: a first comparison based on fluorescence in situ hybridization. Appl. Environ. Microbiol. 65:3721-3726
  20. Pernthhaler J, FO Glockner, S Unterholzner, A Alfreider, R Psenner and R Amann. 1998. Seasonal community and population dynamics of pelagic bacteria and archaea in a high mountain lake. Appl. Environ. Microbiol. 64:4299-4306
  21. 김동주, 홍선희, 안태석. 1999. 소양호에서 세균군집소의 계절적. 수직적 변화. 한국미생물학회지. 35:242-247
  22. Alfreider AJ, R Pernthhaler, B Amann, FO Sattler, A Glockner, A Wille and R Psenner. 1996. Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization. Appl. Environ. Microbiol. 62:2138-2144
  23. Lee YO, JH Park and JK Park. 2005. Microbial characterization of excessive growing biofilm in sewer lines using molecular technique. J. Microbiol. Biotechnol. 15:938-945