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Adhesion Properties of Indigenous Dadih Lactic Acid Bacteria on Human Intestinal Mucosal Surface

  • Dharmawan, Jorry (Food Science and Technology Programme, Department of Chemistry, National University of Singapore) ;
  • Surono, Ingrid S. (Center for the Assessment and Application of Biotechnology, Agency of Assessment and Application Technology) ;
  • Kun, Lee Yuan (Department of Microbiology, Faculty of Medicine, National University of Singapore)
  • Received : 2005.08.04
  • Accepted : 2005.12.20
  • Published : 2006.05.01

Abstract

Dadih is Indonesian traditional fermented buffalo milk believed by the natives to have beneficial effects on human health. This may be due to the probiotic properties possessed by the lactic acid bacteria (LAB) involved in its fermentation process. It was discovered that ten strains of dadih lactic isolates possessed some probiotic properties in vitro. In this study, the adhesion properties of dadih LAB, in comparison with documented probiotic strains, were investigated in vitro by using mucin extracted from human faeces and Caco-2 cells as the models for human intestinal mucosal surface and intestinal cells respectively. The adhesion results showed the distinction of Lactobacillus reuteri IS-27560 in adhering to both mucus layer and Caco-2 cells. The competition assay for adhesion to the mucus layer between dadih LAB and selected pathogens indicated the competence of Lactococcus lactis IS-16183 and Lactobacillus rhamnosus IS-7257 in significantly inhibiting the adhesion of Escherichia coli O157:H7. Accordingly, these two strains may be potential candidates for use as probiotic strains. Overall, the adhesion properties of all dadih LAB strains were relatively comparable to that of Lactobacillus casei Shirota and Lactobacillus rhamnosus GG, the documented probiotic strains.

References

  1. Beachey, E. H. 1981. Bacterial adherence: adhesion-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J. Infect. Dis. 143:325-345 https://doi.org/10.1093/infdis/143.3.325
  2. Bibiloni, R., P. F. Pérez and G. L. De Antoni. 1999. Will a high adhering capacity in a probiotic strain guarantee exclusion of pathogens from intestinal epithelia? Anaerobe 5:519-524 https://doi.org/10.1006/anae.1999.0198
  3. Hosono, A., R. Wardojo and H. Otani. 1990. Binding of amino acid pyrolyzates by lactic acid bacteria isolated from dadih. Lebensm-Wiss. Technol. 23:149-153
  4. Kaur, I. P., K. Chopra and A. Saini. 2002. Probiotics: potential pharmaceutical applications. Eur. J. Pharm. Sci.15:1-9 https://doi.org/10.1016/S0928-0987(01)00209-3
  5. Kirjavainen, P. V., A. C. Ouwehand, E. Isolauri and S. J. Salminen. 1998. The ability of probiotic bacteria to bind to human intestinal mucus. FEMS Microbiol. Lett. 167:185-189 https://doi.org/10.1111/j.1574-6968.1998.tb13226.x
  6. Ouwehand, A. C., P. V. Kirjavainen, C. Shortt and S. J. Salminen. 1999. Probiotics: mechanisms and established effects. Int. Dairy J. 9:43-52 https://doi.org/10.1016/S0958-6946(99)00043-6
  7. Ouwehand, A. C., S. J. Salminen and E. Isolauri. 2002. Probiotics: an overview of beneficial effects. Anton. Leeuw. Int. J. G. 82:279-289
  8. Pato, U., I. S. Surono, Koesnandar and A. Hosono. 2004. Hypocholesterolemic effect of indigenous dadih lactic acid bacteria by deconjugation of bile salts. Asian-Aust. J. Anim. Sci. 17:1741-1745 https://doi.org/10.5713/ajas.2004.1741
  9. Saarela, M., G. Mogensen, R. Fonden, J. Mättö and T. Mattila- Sandholm. 2000. Probiotic bacteria: safety, functional and technological properties. J. Biotechnol. 84:197-215 https://doi.org/10.1016/S0168-1656(00)00375-8
  10. Saarela, M., L. Lähteenmäki, R. Crittenden, S. J. Salminen and T. Mattila-Sandholm. 2002. Gut bacteria and health foods-the european perspective. Int. J. Food Microbiol. 78:99-117 https://doi.org/10.1016/S0168-1605(02)00235-0
  11. Salminen, S. J., E. Isolauri and E. Salminen. 1996. Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Anton. Leeuw. Int. J. G. 70: 347- 358 https://doi.org/10.1007/BF00395941
  12. Salminen, S. J., A. C. Ouwehand and E. Isolauri. 1998. Clinical applications of probiotic bacteria. Int. Dairy J. 8:563-572 https://doi.org/10.1016/S0958-6946(98)00077-6
  13. Salminen, S. J., A. C. Ouwehand, Y. Benno and Y. K. Lee. 1999. Probiotics: how should they be defined? Trends Food Sci. Tech. 10:107-110 https://doi.org/10.1016/S0924-2244(99)00027-8
  14. Surono, I. S. and A. Hosono. 1996. Antimutagenicity of milk cultured with lactic acid bacteria from dadih against mutagenic terasi. Milchwissenschaft 51:493-497
  15. Surono, I. S. 2003. In vitro probiotic properties of indigenous dadih lactic acid bacteria. Asian-Aust. J. Anim. Sci. 16:726- 731 https://doi.org/10.5713/ajas.2003.726
  16. Tuomola, E. M., A. C. Ouwehand and S. J. Salminen. 1999. The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus. FEMS Immunol. Med. Mic. 26:137- 142 https://doi.org/10.1111/j.1574-695X.1999.tb01381.x