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Effect of Forage to Concentrate Ratio and Monensin Supplementation on cis-9, trans-11 Conjugated Linoleic Acid and trans-11 Octadecenoic Acid Concentrations of Ruminal Contents and Plasma in Sheep

  • Zhang, Yuzhi (College of Animal Science and Technology, China Agricultural University) ;
  • Kong, Xianghao (College of Animal Science and Technology, China Agricultural University) ;
  • Zhu, Xiaoping (College of Animal Science and Technology, China Agricultural University) ;
  • Wang, Runlian (College of Animal Science and Technology, China Agricultural University) ;
  • Yan, Yichai (Beijing Chuangxin Kenong Agri-Husbandry Technology) ;
  • Jia, Zhihai (College of Animal Science and Technology, China Agricultural University)
  • Received : 2005.08.04
  • Accepted : 2006.01.03
  • Published : 2006.05.01

Abstract

Twenty-four cannulated Small-tailed Han${\times}$Poll Dorset wethers (BW $47.5{\pm}2.1kg$) were used to determine the effects of forage to concentrate ratio (40:60 vs. 70:30), monensin supplementation (0, 15 or 30 ppm, DM basis) and interactions of these two factors on cis-9, trans-11 conjugated linoleic acid (cis-9, trans-11 CLA) and trans-11 octadecenoic acid (trans11-$C_{18:1}$) concentrations in ruminal contents and plasma in sheep. The experiment was designed as a $2{\times}3$ factorial. The diet contained Chinese wild rye grass hay (Aneurolepidium Chinese), cracked corn, soybean meal, NaCl, limestone and trace mineral premix. Dietary crude fat and linoleic acid ($C_{18:2n-6}$) were adjusted with soybean oil to about 7.0% and 24.0 mg/g (DM basis), respectively. High forage diets increased (p<0.001) the concentrations of trans11-$C_{18:1}$ and cis-9, trans-11 CLA in ruminal contents and plasma. Monensin supplementation increased (p<0.001) the concentration of trans11-$C_{18:1}$ in ruminal contents, but had no effect on that of cis-9, trans-11 CLA. Concentrations of trans11-$C_{18:1}$ (p<0.019) and cis-9, trans-11 CLA (p<0.022) in plasma increased with dietary monensin levels. Interactions of forage: concentrate ratio and monensin level tended to affect the concentrations of trans11-$C_{18:1}$ (p<0.091) and $C_{18:2n-6}$ (p<0.083) in ruminal contents. Increasing forage levels increased the concentrations of trans11-$C_{18:1}$ and cis-9, trans-11 CLA in the rumen. Supplementing with monensin increased the ruminal production of trans11-$C_{18:1}$ and concentrations of trans11-$C_{18:1}$ and cis-9, trans-11 CLA in plasma.

Keywords

cis-9;trans-11 CLA;trans11-$C_{18:1}$;Forage to Concentrate Ratio;Monensin;Rumen;Plasma

References

  1. An, B. K., C. W. Kang, Y. Izumi, Y. Kobayashi and K. Tanaka. 2003. Effects of dietary fat sources on occurrences of conjugated linoleic acid and trans fatty acids in ruminal contents. Asian-Aust. J. Anim. Sci. 16(2):222-256 https://doi.org/10.5713/ajas.2003.222
  2. Beaulieu, A. D., J. K. Drackley and N. R. Merchen. 2002. Concentrations of conjugated linoleic acid(cis-9, trans-11- octadecadienoic acid)are not increased in tissue lipids of cattle fed a high-concentrate diet supplemented with soybean oil. J. Anim. Sci. 80:847-861
  3. Kalscheur, K. F., B. B. Teter, L. S. Piperova and R. A. Erdman. 1997. Effect of dietary forage concentration and buffer addition on duodenal flow of trans-$C_{18:1}$ fatty acids and milk fat production in dairy cows. J. Dairy Sci. 80:2104-2114 https://doi.org/10.3168/jds.S0022-0302(97)76156-3
  4. Martin, S. A. and T. C. Jenkins. 2002. Factors affecting conjugated linoleic acid and trans-C18:1 fatty acid production by mixed ruminal bacteria. J. Anim. Sci. 80:3347-3352 https://doi.org/10.2527/2002.80123347x
  5. Russell, J. B. and R. L. Baldwin. 1979. Comparison of substrate affinities among several rumen bacteria: a possible determinant of rumen bacterial competition. Appl. Environ. Microbiol. 37:531-536
  6. Wang, J. H. and M. K. Song. 2003. pH affects the in vitro formation of cis-9, trans-11 CLA and trans-11 octadecenoic acid by ruminal bacteria when incubated with oilseeds. Asian-Aust. J. Anim. Sci. 16(12):1743-1748 https://doi.org/10.5713/ajas.2003.1743
  7. Wu, Z., O. A. Ohajuruka and D. L. Palmquist. 1991. Ruminal synthesis, biohydrogenation, and digestibility of fatty acids by dairy cows. J. Dairy Sci. 74:3025-3034 https://doi.org/10.3168/jds.S0022-0302(91)78488-9
  8. Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18(2):221-225 https://doi.org/10.5713/ajas.2005.221
  9. Wang, J. H., S. H. Choi and M. K. Song. 2003. Effects of concentrate to roughage ratio on the formation of cis-9, trans- 11 CLA and trans-11-octadecenoic acid in rumen fluid and plasma of sheep when fed high oleic or high linoleic acid oils. Asian-Aust. J. Anim. Sci. 16(12):1604-1609 https://doi.org/10.5713/ajas.2003.1604
  10. Fellner, V., F. D. Sauer and J. K. G. Kramer. 1997. Effect of nigericin, monensin, and tetronasin on biohydrogenation in continuous flow-through ruminal fermenters. J. Dairy Sci. 80:921-928 https://doi.org/10.3168/jds.S0022-0302(97)76015-6
  11. Kim, T. W., N. J. Choi, J. Hwangbo, Jih Tay Hsu, S. S. Lee, M. K. Song, I. J. Seo and Y. J. Kim. 2005. Production of trans-10, cis-12 conjugated linoleic acid by Megasphaera elsdenii YJ-4: physiological roles in the rumen. Asian-Aust. J. Anim. Sci. 18(10):1425-1429 https://doi.org/10.5713/ajas.2005.1425
  12. Qiu, X., M. L. Eastridge, K. E. Griswold and J. L. Firkins. 2004. Effects of substrate, passage rate and pH in continuous culture on flows of conjugated linoleic acid and trans C18:1. J. Dairy Sci. 87:3473-3479 https://doi.org/10.3168/jds.S0022-0302(04)73482-7
  13. Van Nevel, C. and D. I. Demeyer. 1995. Lipolysis and biohydrogenation of soybean oil in the rumen in vitro: Inhibition by antimicrobials. J. Dairy Sci. 78:2797-2806 https://doi.org/10.3168/jds.S0022-0302(95)76910-7
  14. Jenkins, T. C., V. Feller and R. K. McGuffey. 2003. Monensin by fat interactions on trans fatty acids in cultures of mixed ruminal microorganisms grown in continuous fermentors fed corn or barley. J. Dairy Sci. 86:324-330 https://doi.org/10.3168/jds.S0022-0302(03)73609-1
  15. Kim, Y. J. 2003. Partial inhibition of biohydrogenation of linoleic acid can increase the conjugated linoleic acid production of Butyrivibrio fibrisolvens A38. J. Agric. Food. Chem. 51:4258- 4262 https://doi.org/10.1021/jf034057r
  16. Sackmann, J. R., S. K. Duckett, M. H. Gillis, C. E. Realini, A. H. Parks and R. B. Eggelston. 2003. Effects of forage and sunflower oil levels on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets. J. Anim. Sci. 81:3174-3181
  17. Griinari, J. M., B. A. Corl, S. H. Lacy, P. Y. Chouinard, K. V. V. Nurmela and D. E. Bauman. 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by ${\Delta}^9$-desaturase. J. Nut. 130:2285-2291 https://doi.org/10.1093/jn/130.9.2285
  18. Harfoot, C. G. 1981. Lipid metabolism in the rumen. In: Lipid metabolism in ruminant animals, (Ed. W. W. Christie). Pergamon press, New York, pp. 21-55
  19. Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002a. Effect of concentrate level on the formation of conjugated linoelic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseeds in vitro. Asian-Aust. J. Anim. Sci. 15(5):687-694 https://doi.org/10.5713/ajas.2002.687
  20. Griinari, J. M. and D. E. Bauman. 1999. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In: Advances in conjugated linoleic acid research, Volume 1, (Ed. M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, G. J. Nelson and G. J. Nelson). AOCS press, Illinois, Chapter 13, pp. 180-200
  21. Kepler, C. R., W. P. Tucker and S. B. Tove. 1970. Biohydrogenation of unsaturated fatty acids. IV. Substrate specificity and inhibition of linoleate delta 12-cis, delta 11- trans isomerase from Butyrivibrio fibrisolvens. J. Biol. Chem. 245:3612-3620
  22. Khanal, R. C. 2004. Potential health benefits of conjugated linoleic acid (CLA): a review. Asian-Aust. J. Anim. Sci. 17(9):1315-1328 https://doi.org/10.5713/ajas.2004.1315
  23. Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002b. Addition of seed-associated or free linseed oil on the formation of cis-9, trans-11 conjugated linoleic acid and octadecenoic acid by ruminal bacteria in vitro. Asian-Aust. J. Anim. Sci. 15(8):1115-1120 https://doi.org/10.5713/ajas.2002.1115
  24. Kucuk, O., B. W. Hess, P. A. Ludden and D. C. Rule. 2001. Effect of forage:concentrate ratio on ruminal digestion and duodenal flow of fatty acids in ewes. J. Anim. Sci. 79:2233-2240
  25. Choi, S. H. and M. K. Song. 2005. Effect of C18-polyunsaturated fatty acids on their direct incorporation into the rumen bacterial lipids and CLA production in vitro. Asian-Aust. J. Anim. Sci. 18(4):512-515 https://doi.org/10.5713/ajas.2005.512
  26. Wang, J. H. and M. K. Song. 2001. Effect of sources and levels of carbohydrates on fermentation characteristics and biohydrogenation of linoleic acid by rumen bacteria in vitro. Asian-Aust. J. Anim. Sci. 14(1):48-53 https://doi.org/10.5713/ajas.2001.48
  27. Folch, J., M. Lees and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509
  28. NRC. 1985. Nutrient requirements of sheep. (6th ed.). National Academy of Science. Washington, DC

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