DOI QR코드

DOI QR Code

Vascular Endothelial Growth Factor Has Beneficial Effect Independent of Serum Components throughout Oocyte Maturation and Early Embryonic Development in Cattle

  • Luo, Hailing (The College of Animal Science and Technology, China Agricultural University) ;
  • Kimura, Koji (Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science) ;
  • Hirako, Makoto (Department of Research Planning and Coordination, Headquarters of National Agriculture and Bio-oriented Research Organization)
  • Received : 2005.05.28
  • Accepted : 2005.11.24
  • Published : 2006.04.01

Abstract

In our previous studies, we demonstrated that Vascular Endothelial Growth Factor (VEGF) enhances bovine oocyte maturation and early embryonic development in serum supplemented media. In this experiment, to determine the synergistic effect of VEGF with serum components on early embryonic development in vitro in cattle, 1 mg/ml polyvinyl-alcohol (PVA) was replaced with foetal bovine serum (FBS) in maturation and culture media. Bovine oocytes were matured in Synthetic Oviduct Fluid (SOF) supplemented with PVA, PVA+5 ng/ml of VEGF, FBS, or FBS+VEGF. Fertilized oocytes were cultured in the same conditions for 8 days. The development of embryos was examined at 48 h post- insemination and on days 6, 7 and 8. The results were analyzed using repeated measures two- factor ANOVA, in which the effects of VEGF and serum were assigned as two factors. The development rate to 4- to 8-cell embryos at 48 h was significantly higher in the PVA+VEGF group than in the PVA group (44.7% and 31.5%, respectively). However, the highest development rate to 4- to 8-cell embryos was obtained from the FBS+VEGF group (58.8%). On day 8, the blastocyst rates were higher in the PVA+VEGF (22.8%), FBS (32.1%, p<0.05) and FBS+VEGF (42.1%, p<0.05) groups than in the PVA group (17.1%). Two- factor ANOVA of the development rates indicates that VEGF had a significant effect, but had no synergistic effect with serum components on early embryonic development. The results of the present study demonstrate that VEGF improves the in vitro developmental competence of bovine oocytes and/or embryos independent of the effect of serum components.

References

  1. Ferrara, N. and T. Davis-Smyth. 1997. The biology of vascular endothelial growth factor. Endocr. Rev. 18:4-25 https://doi.org/10.1210/er.18.1.4
  2. Gabler, C., A. Einspanier, D. Schams and R. Einspanier. 1999. Expression of vascular endothelial growth factor (VEGF) and its corresponding receptors (flt-1 and flk-1) in the bovine oviduct. Mol. Reprod. Dev. 53:376-383 https://doi.org/10.1002/(SICI)1098-2795(199908)53:4<376::AID-MRD2>3.0.CO;2-Z
  3. Lee, E. S. and Y. Fukui. 1995. Effect of various growth factors in defined culture medium on in vitro development of bovine embryos matured and fertilized in vitro. Theriogenol. 44:71-83 https://doi.org/10.1016/0093-691X(95)00149-3
  4. Trounson, A. O., S. M. Willadsen and L. E. A. Rowson. 1977. Fertilization and development capability of bovine follicular oocytes matured in vitro and in vivo and transferred to the oviducts of rabbits and cows. J. Reprod. Fertil. 51:321-327 https://doi.org/10.1530/jrf.0.0510321
  5. Connolly, D. T. 1991. Vascular permeability factor: a unique regulator of blood vessel function. J. Cell. Biochem. 47:219- 223 https://doi.org/10.1002/jcb.240470306
  6. Einspanier, R., K. Muller, B. Bieser, M. Kosmann and D. Schams. 1999. Differential expression of VEGF in bovine ovarian follicles and first effect of VEGF applied during in vitro maturation of oocytes. J. Reprod. Fertil. (Abstract series) 23:7
  7. Brackett, B. G. and G. Oliphant. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12:260-274 https://doi.org/10.1095/biolreprod12.2.260
  8. Connolly, D. T., J. V. Olander, D. Heuvelman, R. Nelson, R. Monsell, N. Siegel, B. L. Haymore, R. M. Leimgruber and J. Feder. 1989. Human vascular permeability factor. Isolation from U937 cells. J. Biol. Chem. 264:20017-20024
  9. Park, K. W., K. Iga and K. Niwa. 1997. Exposure of bovine oocytes to EGF during maturation allows them to development blastocysts in a chemically-defined medium. Theriogenol. 48:1127-1135 https://doi.org/10.1016/S0093-691X(97)00345-2
  10. Gandolfi, F., T. A. L. Brevini, S. Modina and A. Lauria. 1991. Detection and characterization of a growth factors in bovine oviduct secretions. J. Reprod. Fertil. (Abstract series) 7:6
  11. Heyner, S., N. Shan, R. M. Smith, A. J. Watson and G. A. Schultz. 1993. The role of growth factors in embryo production. Theriogenol. 39:151-161 https://doi.org/10.1016/0093-691X(93)90030-9
  12. Host-Hansen, T., B. Avery, P. Hyttel and T. Greve. 1993. Embryonic growth factor receptors and growth factor secretion by cultured oviduct epithelial cells in cattle (Bos Taurus). Reprod. Domest. Anim. 28:193-194 https://doi.org/10.1111/j.1439-0531.1993.tb00120.x
  13. Gabler, C., D. Schams, A. Einspanier and R. Einspanier. 1996. VEGF, TGF-alpha, TGF-beta in the bovine oviduct and indication of their regulation during the estrous cycle. Arch. Tierzucht. (SPEC. ISSUE) 39:29
  14. Connolly, D. T., D. M. Heuvelman, R. Nelson, J. V. Olander, B. L. Eppley, J. J. Delfino, N. R. Siegel, R. M. Leimgruber and J. Feder. 1989. Tumor vascular permeability factor stimulates endothelial cell growth and angiogenesis. J. Clin. Invest. 84:1470-1478 https://doi.org/10.1172/JCI114322
  15. Gandolfi, F., T. A. L. Brevini, S. Modina and L. Bianchi. 1993. Role of the oviduct during early embryogenesis. Reprod. Domest. Anim. 28:189-192 https://doi.org/10.1111/j.1439-0531.1993.tb00119.x
  16. Ehrenwald, E., R. H. Foote and J. E. Parks. 1990. Bovine oviductal fluid components and their potential role in sperm cholesterol efflux. Mol. Reprod. Dev. 25:195-204 https://doi.org/10.1002/mrd.1080250213
  17. Kita, K. and J. Okumura. 2001. Chicken Insulin-Like Growth Factor-I stimulates protein synthesis of chicken embryo myoblasts cultured in serum-free medium. Asian-Aust. J. Anim. Sci. 14:17-20 https://doi.org/10.5713/ajas.2001.17
  18. Gerena, R. L. and G. J. Killian. 1990. Electrophoretic characterization of proteins in oviduct fluid of cows during the estrous cycle. J. Exp. Zool. 256:113-120 https://doi.org/10.1002/jez.1402560114
  19. Luo, H., K. Kimura, M. Aoki and M. Hirako. 2002b. Vascular Endothelial Growth Factor (VEGF) Promotes the Early Development of Bovine Embryo in the Presence of Cumulus Cells. J. Vet. Med. Sci. 64:967-971 https://doi.org/10.1292/jvms.64.967
  20. Gupta, P. S. S. P., J. P. Ravindra, S. Nandi, H. M. Raghu and K. P. Ramesha. 2005. Isolation of an occyte stimulatory peptide from the ovarian follicular fluid of water buffalo (Bubalus bubalis). Asian-Aust. J. Anim. Sci. 18(11):1557-1563 https://doi.org/10.5713/ajas.2005.1557
  21. Larson, R. C., G. G. Ignotz and W. B. Currie. 1992. Platelet derived growth factor PDGF stimulates development of bovine embryos during the fourth cell cycle. Development 115:821- 826
  22. Luo, H., K. Kimura, M.Aoki and M. Hirako. 2002a. Effects of Vascular Endothelial Growth Factor on Maturation, Fertilization and developmental Competence of Bovine Oocytes. J. Vet. Med. Sci. 64:803-806 https://doi.org/10.1292/jvms.64.803
  23. Im, K. S. and K. W. Park. 1995. Effect of epidermal growth factor on maturation, fertilization and development of bovine follicular oocytes. Theriogenol. 43:209-214 https://doi.org/10.1016/0093-691X(95)92363-E
  24. Keefer, C. L., S. L. Stice, A. A. M. Paprocki and P. Golueke. 1994. In vitro culture of bovine IVM-IVF embryos: Cooperative interaction among embryos and the role of growth factors. Theriogenol. 41:1323-1331 https://doi.org/10.1016/0093-691X(94)90491-Z
  25. Leung, D. W., G. Cachianes, W. J. Kuang, D. V. Goeddel and N. Ferrara. 1989. Vascular endothelial growth factor is a secreted angiogenic mitogen. Sci. 246:1306-1309 https://doi.org/10.1126/science.2479986