Relationship between the Polymorphisms of 5' Regulation Region of Prolactin Gene and Milk Traits in Chinese Holstein Dairy Cows

  • Li, J.T. (Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Wang, A.H. (Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Chen, P. (College of Animal Science & Technology, China Agricultural University) ;
  • Li, H.B. (Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Zhang, C.S. (Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Du, L.X. (Institute of Animal Science, Chinese Academy of Agricultural Sciences)
  • Received : 2005.04.11
  • Accepted : 2005.11.03
  • Published : 2006.04.01


Prolactin (PRL) plays an important role in promoting mammalian mammary gland development, and milk production during lactation. Therefore the PRL gene was chosen as a candidate gene for milk traits in Holstein dairy cows. PCR-SSCP and PCR-RFLP were used to analyze genetic variations in the 5' regulation region of the PRL gene. In this part of the gene, two new polymorphic sites were detected in the Chinese Holstein dairy cows. One was a XbaI-RFLP locus, and the other was an SSCP locus. Statistical analysis showed that the XbaI-RFLP locus and the SSCP locus had a significant positive effect on milk traits.


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