Oestrogenic Activity of Parabens In Vitro Estrogen Assays

에틸, 프로필, 이소프로필, 부틸, 이소부틸 파라벤의 In Vitro 검색시험 연구에서의 내분비독성

  • Lee Sung-Hoon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kim Sun-Jung (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jo Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Ahn Nam-Shik (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Hwang Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • 이성훈 (서울대학교 수의과대학 공중보건학교실) ;
  • 김선중 (서울대학교 수의과대학 공중보건학교실) ;
  • 박정란 (서울대학교 수의과대학 공중보건학교실) ;
  • 조은혜 (서울대학교 수의과대학 공중보건학교실) ;
  • 안남식 (서울대학교 수의과대학 공중보건학교실) ;
  • 박준석 (서울대학교 수의과대학 공중보건학교실) ;
  • 황재웅 (서울대학교 수의과대학 공중보건학교실) ;
  • 정지윤 (공주대학교 산업과학대학 특수동물학과) ;
  • 이영순 (서울대학교 수의과대학 공중보건학교실) ;
  • 강경선 (서울대학교 수의과대학 공중보건학교실)
  • Published : 2006.06.01

Abstract

The use of underarm and body care cosmetics with oestrogenic chemical excipients (particularly the parabens) and the hypothesized association with breast cancer incidence, particularly in women. It is noted that the type of cosmetic product is irrelevant (e.g. antiperspirant/deodorant versus body lotion, moisturizers or sprays versus creams) and attention must focus on issues of actual exposure to chemicals through continued dermal application of body care products and the endocrine/hormonal activity and toxicity of the chemicals in the formulations. To evaluate the estrogenic activities of parabens such as ethylparaben, butylparaben, propylparaben, isobutylparaben and isopropylparaben, we used recombinant yeasts containing the human estrogen receptor [Saccharomyces cerevisiae ER+LYS 8127], human breast cancer MCF-7 cell lines and human estrogen receptor ${\alpha}\;and\;{\beta}$. In E-screen assays, isopropylparaben is the most estrogenic paraben, and in ER competition assay, isobutylparaben is the most estrogenic paraben. We evaluated isopropylparaben was most active in the recombinant yeast assay, followed by propylparaben, ethylparaben, isobutylparaben and butylparaben. Results from this study demonstrate that parabens are observed in human endocrine system. Therefore, we have shown that the parabens is induced the estrogenic activities similar to $17{\beta}$-estradiol and Bisphenol-A.

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