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Short-term Toxicity Assay Based on Daphnid Feeding on the Microalga Scenedesmus subspicatus

  • Lee Sang-Ill (Department of Environmental Engineering, Chung Buk National University) ;
  • Park Jong-Ho (Department of Inland Fisheries Research Institute) ;
  • Lee Won-Ho (Department of Construction & Urban Engineering, Chung Ju National University) ;
  • Yeon Ik-Jun (Department of Environmental Engineering, Chung Ju National University) ;
  • Lee Byoung-Chan (Department of Environmental Engineering, Chung Ju National University) ;
  • Cho Kyu-Seok (Department of Inland Fisheries Research Institute) ;
  • Choi Hyun-Ill (POSCO Engineering & Construction Co., Ltd.)
  • Published : 2006.03.01

Abstract

We developed and evaluated a method of short-term acute toxicity testing based on the feeding behavior of Ceriodaphnia dubia. In prior toxicity tests, neonates of C. dubia were hatched and cultivated with the addition of yeast only for the preparation of the transparent daphnid's gut. Scenedesmus subspicatus was supplied as food after 1 to 6 h of exposure to toxicants. The effects of 1-h and 6-h exposure time on test sensitivity did not significantly differ. A comparison of the short-term l-h acute toxicity test developed in this study to the standard 48-h acute toxicity test using heavy metals, cyanide, and pentachlorophenol indicated that the 1-h test provided an acceptable sensitivity level in toxicity testing of C. dubia..

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