Application of PCR for diagnosis of porcine circovirus type 2

돼지 써코바이러스 2형의 진단을 위한 PCR법 적용

  • Park Hyo-Sun (Dangjin branch of the Chungnam Livestock & Veterinary Research Institute) ;
  • Lee Hyo-Sang (Dangjin branch of the Chungnam Livestock & Veterinary Research Institute) ;
  • Na Ki-Bok (Dangjin branch of the Chungnam Livestock & Veterinary Research Institute) ;
  • Lee Kwan-Bok (Dangjin branch of the Chungnam Livestock & Veterinary Research Institute) ;
  • Kang Su-Jung (Dangjin branch of the Chungnam Livestock & Veterinary Research Institute) ;
  • Moon Sun-Hwa (Dangjin branch of the Chungnam Livestock & Veterinary Research Institute)
  • 박효선 (충청남도 축산위생연구소 당진지소) ;
  • 이효상 (충청남도 축산위생연구소 당진지소) ;
  • 나기복 (충청남도 축산위생연구소 당진지소) ;
  • 이관복 (충청남도 축산위생연구소 당진지소) ;
  • 강수정 (충청남도 축산위생연구소 당진지소) ;
  • 문순화 (충청남도 축산위생연구소 당진지소)
  • Published : 2006.03.01

Abstract

Porcine circovirus (PCV) is a small, nonenveloped virus that contains a single-stranded circular DNA genome of about 1.76 kb and belongs to the family circoviridae. The PCV-2 has been incriminated as the cause of post-weaning multisystemic wasting syndrome (PMWS) , an emerging disease in pigs. In the present study, a PCR assay was applied to detect PCV-2 in tissue samples. The presence of PCV-2 antigen in the porcine tissues was confirmed by indirect immunofluorescence (IIF) with PCV-2 specific monoclonal antibodies. And then DNA extracted from PCV-2 positive tissues was used as a template. One oligonucleotide primer suitable for PCR was selected from a published PCV-2 sequence (Genbank). Amplified PCR product was detected the same fragment lengths of 416 bp as a control. Based on these results, it was suggested that the PCR is a simple and sensitive method for support diagnostic purposes.

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