Establishment of the Convenient Boar Semen Freezing Method and Assessment of Viability in Frozen/Thawed Boar Semen

돼지 정액의 간편 동결 방법 확립과 동결 정액의 융해 후 생존성 평가

  • Kim Seong-Kon (College of Animal Life Sciences, Kangwon National University) ;
  • Jang Hyun-Yong (College of Animal Life Sciences, Kangwon National University) ;
  • Park Dong-Heon (College of Animal Life Sciences, Kangwon National University) ;
  • Park Chun-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Cheong Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Kim Choung-Ik (College of Animal Life Sciences, Kangwon National University) ;
  • Yang Boo-Keun (College of Animal Life Sciences, Kangwon National University)
  • 김성곤 (강원대학교 동물생명과학대학) ;
  • 장현용 (강원대학교 동물생명과학대학) ;
  • 박동헌 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의학부대학) ;
  • 김정익 (강원대학교 동물생명과학대학) ;
  • 양부근 (강원대학교 동물생명과학대학)
  • Published : 2006.03.01

Abstract

This study was conducted to establish a convenient freezing method of boar semen. Boar semen was cooled until $5^{\circ}C$ for 3 hrs using cell freezer and loaded into straws. Semen straws were frozen in different steps in strofoam box filled with $LN_2$. Highest sperm viability (54.0%) was obtained by 1-step freezing(holding at 10 cm height from the surface of $LN_2$ for 10 min). Sperm viability increased by holding at $-102^{\circ}C$ for 10min (74.0%, P<0.05). In thawing regime, sperm viability was significantly higher in $37^{\circ}C$ group than in $52^{\circ}C$ group. The sperm characteristics did not differ between 1-step and 3-step. After IVF using frozen-thawed boar semen, developmental rate of embryos to the morula+blastocyst stage was in 1-step freezing group than that of 3-step freezing group (27.5 vs 14.7%, P<0.05). The result shows that the 1-step freezing with holding at $-102^{\circ}C$ for 10min before plunging into $LN_2$ is a convenient and easy freezing method for boar semen.

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