DOI QR코드

DOI QR Code

Effects of Electron Beam Irradiation on Functional and Other Associated Properties of Pork Myofibrillar Salt-Soluble Proteins

  • Koh, Kwang-Hwan ;
  • Lee, Sam-Pin ;
  • Whang, Key
  • Published : 2006.03.01

Abstract

Ground pork was irradiated with an electron beam (e-beam) at a dose of 0, 1.5, 3, 5 and 10 kGy and the changes in various functional and other associated properties of salt-soluble proteins extracted from the pork were evaluated. Irradiation did not affect turbidity and the disulfide content of pork salt-soluble protein, but the content of sulfhydryls and the hydrophobocity of salt-soluble protein increased. This indicates that protein degradation occurred when the pork was e-beam irradiated and that the sulfhydryls and hydrophobic moieties buried inside the proteins were exposed to the outside environment. However, these degraded protein molecules did not form large protein aggregates through disulfide bridges. The emulsifying capacity of the pork increased with irradiation, which could be the result from increased hydrophobicity of pork salt-soluble protein. Water holding capacity of pork was not affected bye-beam irradiation.焈돀—⨀堘?⨀ࠥƗ⨀䤈돐⠥Ɨ⨀??⨀〥Ɨ⨀℈덐䀥Ɨ⨀ 耔Ɨ⨀

Keywords

electron beam;pork salt-soluble proteins;functional properties

References

  1. Thayer DW, Rajkowski KT. 1999. Developments in irra-diation of fresh fruits and vegetables. Food Technol 53: 62-65
  2. Shamsuzzanman K, Lutch L, Chuaqui-Offermanns N. 1995. Effects of combined electron-bean irradiation and sous- vide treatments on microbiological and other qualities of chicken breast meat. J Food Prot 58: 497-501 https://doi.org/10.4315/0362-028X-58.5.497
  3. Lee MK, Lee MH, Kwon JH. 1998. Sterilizing effect of electron beam on ginseng powders. Kor J Food Sci Technol 30: 1362-1366
  4. Kim WS, Chung MS, Ko YT. 1998. Effect of low dose gamma ray and electron beam irradiation on the growth of microorganism beef during refrigerated storage. Kor J Food Sci 18: 232-239
  5. Farkas J. 1998. Irradiation as a method for decontaminating food. Intl J Food Microbiol 44: 189-204 https://doi.org/10.1016/S0168-1605(98)00132-9
  6. Kwon H, Lee JE, Kwon JH. 2001. Effects of electron beam irradiation on microbiological and physicochemical qual-ities of powdered Meju and soybean paste. Food Sci Biotechnol 10: 95-100
  7. Nanke KE, Sebranek JG, Olson DG. 1998. Color characteristics of irradiated vacuum-packaged pork, beef and turkey. J Food Sci 63: 1001-1006 https://doi.org/10.1111/j.1365-2621.1998.tb15842.x
  8. Ahn DU, Olson DG, Jo C, Chen X, Wu C, Lee JI. 1998a. Effect of muscle type, packaging, and irradiation on lipid oxidation, volatile production, and color in raw pork patties. Meat Sci 49: 27-39 https://doi.org/10.1016/S0309-1740(97)00101-0
  9. Nanke KE, Sebranek JG, Olson DG. 1999. Color characteristics of irradiated, aerobically packaged pork, beef, and turkey. J Food Sci 64: 272-278 https://doi.org/10.1111/j.1365-2621.1999.tb15881.x
  10. Ahn DU, Jo C, Olson DG. 2000. Analysis of volatile components and the sensory characteristics of irradiated raw pork. Meat Sci 54: 209-215 https://doi.org/10.1016/S0309-1740(99)00081-9
  11. Nam KC, Ahn DU, Jo C. 2001. Lipid oxidation, color, vol-atiles, and sensory characteristics of aerobically packaged and irradiated pork with different ultimate pH. J Food Sci 66: 1225-1229 https://doi.org/10.1111/j.1365-2621.2001.tb16109.x
  12. Koh KH, Whang K. 2002. Effect of electron beam irradi-ation on the oxidative and microbiological stability of ground pork during storage. Kor J Food Sci Ani Resour 22: 316-321
  13. Whang K. 2002. Combination effect of modified atmosphere packaging and electron beam irradiation on the oxidative and microbiological stability of ground pork during storage. Kor J Food Sci Ani Resour 22: 322-329
  14. Ahn DU, Sell JL, Jo C, Chen X, Wu C, Lee JI. 1998. Ef-fects of dietary vitamin E supplementation on lipid oxi-dation and volatiles content of irradiated, cooked turkey meat patties with different packaging. Poult Sci 77: 912-920 https://doi.org/10.1093/ps/77.6.912
  15. Ahn DU, Sell JL, Jeffery C, Chen X, Wu C, Lee JI. 1997. Dietary vitamin E affects lipid oxidation and total volatiles of irradiated raw turkey meat. J Food Sci 62: 954-958 https://doi.org/10.1111/j.1365-2621.1997.tb15014.x
  16. Hermelstein NH. 2000. E-beam irradiated beef reaches the market. Papaya and gamma-irradiated beef to follow. Food Technol 54: 88-92
  17. Judge MD, Aberle ED, Forrert JC, Hedrick FB, Merkel RA. 1989. Principles of meat science. Kendall/Hunt Publish commpany, Dubuque, Iowa. p 125
  18. Chan JK, Gill TA, Paulson AT. 1993. Thermal aggregation of myosin subfragment from cod and herring. J Food Sci 58: 1057-1061 https://doi.org/10.1111/j.1365-2621.1993.tb06111.x
  19. Ellman GD. 1959. Tissue sulfhydryl groups. Arch Biochem Biophys 82: 70-77 https://doi.org/10.1016/0003-9861(59)90090-6
  20. Thanhauser TW, Konishi YS, Scheraga HA. 1984. Sensitive quantitative analysis of disulfide bonds in polypeptide and proteins. Anal Biochem 138: 181-188 https://doi.org/10.1016/0003-2697(84)90786-3
  21. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. 1951. Protein measurements with Folin phenol reagent. J Biol Chem 193: 265-275
  22. Park BY, Yoo YM, Cho SY, Chae HS, Kim JH, An JN, Lee JM, Choi YI, Yun SG. 2001. Changes of physico-chemical compositions in domestic broiler chickens of different marketing standard. Kor J Food Sci Anim Res 21: 337-344
  23. Gill TA, Conway JT. 1989. Thermal aggregation of cod muscle proteins using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide as a zero-length cross-linker. Agric Biol Chem 53: 2553-2559 https://doi.org/10.1271/bbb1961.53.2553
  24. Lee SH, Lee SH, Song KB. 2002. Effect of gamma-irradiation on the molecular properties of blood plasma proteins. Nutraceuticals & Food 7: 184-187 https://doi.org/10.3746/jfn.2002.7.2.184
  25. Jaczynski J, Park JW. 2004. Physicochemical changes in Alaska Pollock surimi and surimi gel as affected by electron beam. J Food Sci 69: 53-57 https://doi.org/10.1111/j.1365-2621.2004.tb17855.x
  26. Ishizaki S, Harada M, Iso N, Taguchi T. 1993. Effect of ultraviolet radiation on the rheological properties of thermal gels from sardine and pork meat pastes. Nippon Suisan Gakkaishi 59: 1219-1224 https://doi.org/10.2331/suisan.59.1219
  27. Chan JK, Gill TA, Paulson AT. 1992. The dynamics of thermal denaturation of fish myosins. Food Res Intern 25: 117-120 https://doi.org/10.1016/0963-9969(92)90152-U
  28. Johnson J, Marcotte M. 1999. Irradiation control of insect pests of dried fruits and walnuts. Food Technol 53: 46-51
  29. Whang K. 2003. Effect of different conveyer speed of electron beam irradiation on the oxidative and microbiological stability of ground pork during refrigeration. Kor J Food Sci Ani Resour 23: 50-55
  30. Bagorogoza K, Bowers J, Okot-Kotber M. 2001. The effect of irradiation and modified atmosphere packaging on the quality of intact chill-stored turkey breast. J Food Sci 66: 367-372 https://doi.org/10.1111/j.1365-2621.2001.tb11348.x
  31. Du M, Nam KC, Ahn DU. 2001. Cholesterol and lipid oxidation products in cooked meat as affected by raw-meat packaging and irradiation by cooked meat packaging and storage time. J Food Sci 66: 1396-1401 https://doi.org/10.1111/j.1365-2621.2001.tb15221.x
  32. Ahn DU, Olson DG, Jo C, Love J, Jin SK. 1999. Volatiles production and lipid oxidation in irradiated cooked saus-age as related to packaging and storage. J Food Sci 64:  226-229 https://doi.org/10.1111/j.1365-2621.1999.tb15870.x
  33. Luchsinger SE, Kropf DH, Garcia-Zepeda CM, Hunt MC, Marsden JL, Rubiocanas EJ, Kastner CL, Kuecher WG, Mata T. 1996. Color and oxidative rancidity of gamma and electron beam irradiated boneless pork chops. J Food Sci 61: 1000-1005, 1093 https://doi.org/10.1111/j.1365-2621.1996.tb10920.x
  34. Lee M, Sebranker K, Parrish Jr FC. 1996. Accelerated post-mortem aging of beef utilizing electron beam irradiation and modified atmosphere packaging. J Food Sci 61: 133-136, 141 https://doi.org/10.1111/j.1365-2621.1996.tb14742.x
  35. Whang K, Jeong DK, Kim HI. 2005. Changes of SDS-PAGE pattern of pork myofibrillar proteins induced by electron beam irradiation. J Food Sci Nutr 10: 378-381 https://doi.org/10.3746/jfn.2005.10.4.378
  36. Hashim IB, Resurrecccion AVA, MacWatters KH. 1995. Disruptive sensory analysis of irradiated frozen or refrigerated chicken. J Food Sci 60: 664-666 https://doi.org/10.1111/j.1365-2621.1995.tb06202.x
  37. Hansen TJ, Chen G, Shieh JJ. 1987. Volatiles in skin of low-dose irradiated fresh chicken. J Food Sci 52: 1180-1182 https://doi.org/10.1111/j.1365-2621.1987.tb14038.x
  38. Heath JL, Owens S, Tesch S, Hannah KW. 1990. Effect of high-energy electron radiation of chicken on thio-barbituric acid values, shear values, odor, and cook yield. Poult Sci 69: 313-319 https://doi.org/10.3382/ps.0690313
  39. Olson DC. 1998. Irradiation of food. Food Technol 52: 56-65
  40. Damodaran S. 1985. Estimation of disulfide bonds using 2-nitro-5-thiosulfobenzoic acid: Limitations. Anal Biochem 145: 200-204 https://doi.org/10.1016/0003-2697(85)90348-3
  41. Ahn DU, Olson DG, Lee JI, Jo C, Wu C, Chen X. 1998b. Packaging and irradiation effects on lipid oxidation and volatiles in pork patties. J Food Sci 63: 15-19 https://doi.org/10.1111/j.1365-2621.1998.tb15665.x
  42. Davies KJA, Delsignore ME. 1987. Protein damage and degradation by oxygen radical. Modification of secondary structure and tertiary structure. J Biol Chem 262: 9908-9913
  43. Li-Chan E, Nakai S, Wood D. 1985. Relationship between functional (fat-binding, emulsifying) and physicochemical properties of muscle proteins. Effects of heating, freezing, pH and species. J Food Sci 50: 1034-1038 https://doi.org/10.1111/j.1365-2621.1985.tb13006.x

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