잿빛 곰팡이병원균 Botrytis cinerea에 대한 Bacillus sp. KMU-1011의 항진균활성

Antifungal Activity of Bacillus sp. KMU-1011 Against Gray Mold Causing Botrytis cinerea

  • Park Sung-Min (Department of Microbiology, Keimyung University) ;
  • Kim Hyun-Soo (Department of Microbiology, Keimyung University) ;
  • Yu Tae-Shick (Department of Microbiology, Keimyung University)
  • 발행 : 2006.03.01

초록

핀란드의 Lappeenranta 지역에 위치하는 Saimaa 호수가의 토양시료로부터 분리된 Bacillus sp. KMU-1011를 이용하여 많은 작물에 잿빛 곰팡이병을 유발하는 Botrytis cinerea KACC 40573에 대한 항진균 활성을 위한 배양조건을 조사하였다. 항진균 물질의 생산을 위한 기본배지로 nutrient broth를 사용하였으며 탄소원으로 1.0% glucose와 질소원으로 1.0% polypeptone를 첨가하였을 때 가장 높은 항진균 활성을 나타내었다. 배양조건으로는 $24^{\circ}C$, 180 rpm, 48시간 배양하였을 때 가장 높은 항진균 활성을 나타내었다. Ammonium sulfate를 $30{\sim}60%$ 첨가하여 항진균 물질을 회수하였을 때 가장 양호한 항진균 활성을 나타내었으며, chloroform을 이용하여 배양액 중에 존재하는 항진균 물질을 회수하여 다양한 작물병원성 곰팡이에 대한 spectrum을 조사한 결과, 잿빛곰팡이병, 고추 탄저병, 참외 탄저병, 수박등의 박과작물의 덩굴마름병, 토마토 시들음병, 글라디올러스 마른썩음병, 토마토 질병, 수박 덩굴쪼김병. 수박 질병, 사과나무 역병, 벼 잎집무늬마름병, 참외 줄기썩음병, 그리고 고추 균핵병에 대하여 양호한 항진균 활성을 나타내는 것으로 조사되었다.

키워드

Botrytis cinerea;antifungal activity;Bacillus sp. KMU-1011

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