Production of an Antihyperlipemial HMG-CoA Reductase Inhibitor from Bacillus cereus D-3

Bacillus cereus D-3로부터 항고지혈증 HMG-CoA Reductase 저해제의 생산

  • Lee Dae-Hyoung (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Lee Jae-Won (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Jeong Jae-Hong (Agriculture Venture Team. Chungnam Agriculture Techno-Park) ;
  • Lee Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
  • 이대형 (배재대학교 생명유전공학과) ;
  • 이재원 (배재대학교 생명유전공학과) ;
  • 정재홍 (충남농업테크노파크 벤처농업육성팀) ;
  • 이종수 (배재대학교 생명유전공학과)
  • Published : 2006.03.01

Abstract

For the purpose of production of a novel antihyperlipemial HMG-CoA reductase inhibitor from bacteria, a bacterium which showed the highest HMG-CoA reductase inhibitory activity was isolated from traditional Doenjang. This strain was identified as Bacillus cereus (D-3) based on its microbiological characteristics and 165 rRNA sequence analysis. The maximal HMG-CoA reductase inhibitor production from Bacillus cereus D-3 was obtained by cultivation in a Glucose-CSL broth containing 2% glucose, 0.6% corn steep liquor, $0.04%\;K_{2}HPO_4$ and $0.05%\;KH_{2}PO_4$ at $30^{\circ}C$ for 36 h. The final HMG-CoA reductase inhibitory activity under the above conditions was 39.4%.

Keywords

Antihyperlipemial substance;HMG-CoA reductase inhibitor;Bacillus cereus D-3

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