Production of an Antihyperlipemial HMG-CoA Reductase Inhibitor from Bacillus cereus D-3

Bacillus cereus D-3로부터 항고지혈증 HMG-CoA Reductase 저해제의 생산

  • Lee Dae-Hyoung (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Lee Jae-Won (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Jeong Jae-Hong (Agriculture Venture Team. Chungnam Agriculture Techno-Park) ;
  • Lee Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
  • 이대형 (배재대학교 생명유전공학과) ;
  • 이재원 (배재대학교 생명유전공학과) ;
  • 정재홍 (충남농업테크노파크 벤처농업육성팀) ;
  • 이종수 (배재대학교 생명유전공학과)
  • Published : 2006.03.01


For the purpose of production of a novel antihyperlipemial HMG-CoA reductase inhibitor from bacteria, a bacterium which showed the highest HMG-CoA reductase inhibitory activity was isolated from traditional Doenjang. This strain was identified as Bacillus cereus (D-3) based on its microbiological characteristics and 165 rRNA sequence analysis. The maximal HMG-CoA reductase inhibitor production from Bacillus cereus D-3 was obtained by cultivation in a Glucose-CSL broth containing 2% glucose, 0.6% corn steep liquor, $0.04%\;K_{2}HPO_4$ and $0.05%\;KH_{2}PO_4$ at $30^{\circ}C$ for 36 h. The final HMG-CoA reductase inhibitory activity under the above conditions was 39.4%.


Antihyperlipemial substance;HMG-CoA reductase inhibitor;Bacillus cereus D-3


  1. Cushman, D. W. and H. S. Cheung. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacal. 20: 1637-1648
  2. Frimpong, K. and V. W. Rodwell. 1994. Catalysis by Syrian hamster 3-hydroxy- 3-methylreductase. Proposed roles of histidine 865, glutamate 558, and aspartate 766. J. Biol. Chem. 269: 11478-11483
  3. Lee, H. J. and M. S. Choi, 1999. Measurement of inhibitory activities on 3-hydroxy-3-methylglutaryl CoA reductase and Acyl-CoA:Cholesterol acyltransferase by various plant extracts in vitro. J. Kor. Soc. Food Sci. Nutr. 28: 958-962
  4. Lee, J. S., S. H. Yi, S. J. Kwon, C. Ahn, and J. Y. Yoo 1997. Enzyme activities and physiological ti.mctionality of yeasts from traditional Meju. J. Kor. Appl. Microbiol. Biotechnol. 25: 448-453
  5. Bachar, D. A., C. V. Stauffacher, and V. W. Rodwell. 1999. Investigation of the Iysines of Syrian hamster 3-hydroxy-3methylgiutaryl Coenzme A reductase. Biochemistry 38: 15848-15852
  6. Endo, A. 1993. HMG-CoA reductase inhibitors. Natural Products Reports 10: 541-550
  7. Kim, H. J., D. H. Lee, Y. Y. Hwang, K. S. Lee, and J. S. Lee. 2005. Characterization of ${\beta}$-Hydroxy-${\beta}$-Methylglutaryl Coenzyme A reductase inhibitor from Pueraria thunbergiana. J. Agric. Food Chem. 53: 5882-5888
  8. Yoo, S. K., S. M. Kang, and U. S. Noh. 2000. Quality properties on soy bean pastes made with microorganisms isolated from traditional soy bean pastes. Kor. J. Food Sci. Technol. 32: 1266-1270
  9. Bradford, M. M. 1976. A rapid and sensitive methods for the qualification of microgram quantities of protein utilizing the principle of protein'dye binding. Anal. Biochem. 7: 248-254
  10. Lee, J. S., Y. J. Choi, S. J. Kwon, J. Y. Yoo, and D. H. Chung. 1996. Screening and characterization of osmotolerant and gas-producing yeasts from traditional Doenjang and Kochujang. Food Biotechnol. 5: 54-58
  11. Fayek, K. J. and S. T. El-Sayed. 1980. Purification and properties of tibtinolytic enzyme from Bacillus subtilis. Zeit. Allgem. Mikrobiol., 20: 375-382
  12. Kim, Y. S., E. A. lung, l. E. Shin, J. C. Chang, H. K. Yang, N. J. Kim, K. H. Cho, H. S. Bae, S. K. Moon, and D. H. Kim. 2002. Daio-Orengedok:uto inhibits HMG-CoA reductase and pancereatic lipase. Biol. Pharm. Bull. 25: 1442-1445
  13. Lee, S. Y. 1990. Cholesterol. Shinkwang Publish Co. Seoul
  14. Claus, D. and R. C. W. Berkeley. 1986. Genus Bacillus, p 1105-1139. In Sneath, P. H. A., N. S. Mair, M. E. Sharpe, and J. G. Holt (ed.), Bergey's Manual of Systematic Bacteriology, vol. 2. Williams and Wilkins. Baltimore. USA
  15. Gerhardt, P., R. G. E. Murray, R. N. Costilow, E.W. Nester, W. A. Wood, N.R. Krieg, and G. B. Phillips. 1981. p. 407-450, Manual of Methods for General Bacteriology. American Soc. for Microbiol. USA
  16. Ha, T. Y., J. J. Cho, and S. H. Lee. 1998. Screening of HMG-CoA reductase inhibitory activity of ethanol and methanol extracts from cereals and regumes. Korean J. Food Sci. Technolo 30: 224-229
  17. Endo, A. 2004. The discovery and development of HMGCoA reductase inhibitors. Atherosclerosis Suppl. 5: 67-80
  18. Kim, H. J. 2003. Production and characterization of ${\beta}$Hydroxy-${\beta}$-Methylglutaryl Coenzyme A reductase inhibitor from Pueraria thunbergiana Benth. MS thesis of Paichai university. graduate school
  19. Sung, J. H., S. J. Choi, S. W. Lee, K. H. Park, and T. W. Moon. 2004. Isoflavones found in Korean soybean paste as 3-hydroxy-3-methylglutaryl Coenzyme A reductase inhibitors. Biosci. Biotechnol. Biochem. 68: 1051-1058
  20. Kleinsek, D. A. R. E. Dugan, T. A. Baker, and J. W. Porter. 1981. 3-hydroxy-3-methylglutaryl coenzyme A reduct ase from rat liver. Method Enzymol. 71: 462-479