Classification of Listeria monocytogenes Isolates from Korean Domestic Foods Using Random Amplification of Polymorphic DNA and Serotyping Analysis

Random Amplification of Polymorphic DNA와 혈청학적 분석을 이용한 국내식품에서 분리한 Listeria monocytogenes의 분류

  • Kim Hyun-Joong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Park Si-Hong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim Hae-Yeong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
  • 김현중 (경희대학교 생명자원과학연구소 및 생명과학대학 식품생명공학과) ;
  • 박시홍 (경희대학교 생명자원과학연구소 및 생명과학대학 식품생명공학과) ;
  • 김해영 (경희대학교 생명자원과학연구소 및 생명과학대학 식품생명공학과)
  • Published : 2006.03.01

Abstract

Molecular subtyping of Listeria monocytogenes, including type strains and isolates from Korean foods, were performed using random amplification of polymorphic DNA (RAPD). Each Listeria species showed specific RAPD band patterns, and L. monocytogenes serotypes and isolates were divided into two clusters. RAPD results showed that L. monocytogenes isolates from Korean foods were divided into two groups. Group I contained L. monocytogenes serotypes 1/2b and 4b, whereas Group II contained serotypes 1/2a and 1/2c. These results suggested RAPD as possible subtyping methods for Listeria species. Also, RAPD Results showed significant correlation between molecular subtyping and serotyping of L. monocytogenes, and classified two different groups of L. monocytogenes isolated from Korean foods.

Keywords

Classification;Listeria;pathogen;Random Amplification of Polymorphic DNA;serotyping

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