Cloning and Characterization of the Paraquat Resistance-Related Genes from Ochrobactrum anthropi JW-2

Ochrobactrum anthropi JW-2 유래의 Paraquat 내성유전자 PqrA의 주변 유전자군 분석

  • Bae Eun-Kyung (Biotechnology Division, Korea Forest Research Institute) ;
  • Lee Hyo-Shin (Biotechnology Division, Korea Forest Research Institute) ;
  • Won Sung-Hye (Division of Animal Science and Technology, College of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee Byung-Hyun (Division of Animal Science and Technology, College of Agriculture and Life Science, Gyeongsang National University)
  • 배은경 (국립산림과학원 생물공학과) ;
  • 이효신 (국립산림과학원 생물공학과) ;
  • 원성혜 (경상대학교 동물자원과학부) ;
  • 이병현 (경상대학교 동물자원과학부)
  • Published : 2006.03.01

Abstract

A 4,971 bp chromosomal DNA fragment containing the pqrA, paraquat resistance gene, was cloned from Ochrobactrum anthropi JW-2, and the complete nucleotide sequence was determined. Nucleotide and deduced amino acid sequences of the fragment revealed the presence of 4 complete ORFs (orf2, pqrA, orf3, orf4) and two incomplete ORFs(orf1, orf5). Orf1, pqrA, orf4 and orf5 exists at the direct strand but orf2 and orf3 exists at the reverse complementary strand. Orf1 which of incomplete sequences without start codon shares homology with ATP binding region of the response regulator receiver. Orf2 shares high homology with members of the tetR family of transcriptional repressor which have a helix-turn-helix (H-T-H) motif. Therefore, the orf2 is predicted as a transcriptional repressor of pqrA and is designated as pqrR2. Orf3 shares high homology with the members of the lysR family acting as a transcriptional activator which have both of a H-T-H motif at the N-terminal region and substrate binding domain at the C-terminal region. Therefore, the orf3 is predicted as a transcriptional activator of pqrA and is designated as pqrR1. Orf4 shows homology with the periplasmic substrate-binding protein of amino acid ABC transporter. Orf5 which of incomplete sequences without stop codon revealed the homology with the permeases protein of amino acid ABC transporter.

Keywords

Paraquat;paraquat resistance-related genes;Ochrobactrum anthropi JW-2

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