Lessons from the Sea : Genome Sequence of an Algicidal Marine Bacterium Hahella chehuensis

적조 살상 해양 미생물 Hahella chejuensis의 유전체 구조

  • Jeong Hae-Young (Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Yoon Sung-Ho (Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee Hong-Kum (Korea Polar Research Institute, Korea Ocean Research and Development (KORDI)) ;
  • Oh Tae-Kwang (21C Frontier Microbial Genomics and Applications Center, KRIBB) ;
  • Kim Ji-Hyun (Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 정해영 (한국생명공학연구원 시스템미생물연구센터) ;
  • 윤성호 (한국생명공학연구원 시스템미생물연구센터) ;
  • 이홍금 (한국해양연구원 부설 극지연구소) ;
  • 오태광 (21C프론티어 미생물유전체활용기술개발사업단) ;
  • 김지현 (한국생명공학연구원 시스템미생물연구센터)
  • Published : 2006.03.01

Abstract

Harmful algal blooms (HABs or red tides), caused by uncontrolled proliferation of marine phytoplankton, impose a severe environmental problem and occasionally threaten even public health. We sequenced the genome of an EPS-producing marine bacterium Hahella chejuensis that produces a red pigment with the lytic activity against red-tide dinoflagellates at parts per billion level. H. chejuensis is the first sequenced species among algicidal bacteria as well as in the order Oceanospirillales. Sequence analysis indicated a distant relationship to the Pseudomonas group. Its 7.2-megabase genome encodes basic metabolic functions and a large number of proteins involved in regulation or transport. One of the prominent features of the H. chejuensis genome is a multitude of genes of functional equivalence or of possible foreign origin. A significant proportion (${\sim}23%$) of the genome appears to be of foreign origin, i.e. genomic islands, which encode genes for biosynthesis of exopolysaccharides, toxins, polyketides or non-ribosomal peptides, iron utilization, motility, type III protein secretion and pigment production. Molecular structure of the algicidal pigment was determined to be prodigiosin by LC-ESI-MS/MS and NMR analyses. The genomics-based research on H. chejuensis opens a new possibility for controlling algal blooms by exploiting biotic interactions in the natural environment and provides a model in marine bioprospecting through genome research.

Keywords

Harmful algal bloom (HAB);Hahella chejuensis;prodigiosin;algicidal compound;genome sequencing

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