Effects of Water Extracts from Chaenomeles sinensis, Polygonum cuspidatum and Boswellia carterii on LPS-Induced MMP-9 Activation in Raw 264.7 Cell

목과(木瓜), 호장근(虎杖根) 및 유향(乳香) 추출물이 Raw 264.7 cell에서 LPS로 유도된 MMP-9 의 활성에 미치는 영향

  • Lee Tae-Jin (Department of Immunology, College of Medicine, Keimyung University) ;
  • Kim Yeoun-Hee (Department of Immunology, College of Medicine, Keimyung University) ;
  • Shu Seong-Il (Department of Microbiology, College of Medicine, Keimyung University) ;
  • Shin Sang-Woo (College of Oriental Medicine, Daegu Haay University) ;
  • Kim Sang-Chan (College of Oriental Medicine, Daegu Haay University) ;
  • Kwon Young-Kyu (College of Oriental Medicine, Daegu Haay University) ;
  • Park Jong-Wook (Department of Immunology, College of Medicine, Keimyung University) ;
  • Kwon Taeg-Kyu (Department of Immunology, College of Medicine, Keimyung University)
  • 이태진 (계명대학교 의과대학 면역학교실) ;
  • 김연희 (계명대학교 의과대학 면역학교실) ;
  • 서성일 (계명대학교 의과대학 미생물학교실) ;
  • 신상우 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학) ;
  • 권영규 (대구한의대학교 한의과대학) ;
  • 박종욱 (계명대학교 의과대학 면역학교실) ;
  • 권택규 (계명대학교 의과대학 면역학교실)
  • Published : 2006.02.01


Matrix metalloproteinase-9 (MMP-9) is considered to be an important component in the progression of inflammation. Monocytes/macrophages are prominent at inflammation sites, and activation of these cells by stimulants such as lipopolysaccharide (LPS) leads to the production of significant amounts of MMP-9. Here, we show that LPS-induced MMP-9 production and activation was inhibited by the water extract from the fruit of Chaenomeles sinensis (CS), the root of Polygonum cuspidatum (PC), but increased by the extract from Boswellia carterii (BC). To investigate the mechanism by which those extracts inhibits MMP-9 activation, we examined the level of MMP-9 mRNA expression. We observed a significant change in the MMP-9 expression between LPS alone and LPS plus Chaenomeles sinensis and Polygonum cuspidatum extracts-treated cells. In addition, LPS significantly up-regulated MMP-9 promoter activity in Raw 264.7 cells, which was attenuated by the CS and PS extracts. However, water extracts from Boswellia carterii increased MMP-9 expression and MMP-9 promoter activity which were induced by LPS treatment in Raw 264.7 cells. These data suggest that water extracts from Chaenomeles sinensis and Polygonum cuspidatum can modulate anti-inflammatory immune response, which may be in part associated with the regulation of MMP-9 production and/or activation through the regulation of MMP-9 expression in mouse macrophage cells.


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