Study on the Anti-angiogenic Activity of Ethanol Extract of Bojungbangam-tang

보정방암탕 에타놀층의 혈관형성 저해작용에 관한 연구

  • Lee Eun-Ok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Shim Beom-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Surh Young-Joon (College of Pharmacy, Seoul National University) ;
  • Jeon Byung-Hun (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ahn Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Kim Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
  • 이은옥 (경희대학교 한의과대학 병리학교실) ;
  • 심범상 (경희대학교 한의과대학 병리학교실) ;
  • 서영준 (서울대학교 약학대학 생화학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 안규석 (경희대학교 한의과대학 병리학교실) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실)
  • Published : 2006.02.01

Abstract

The anti-angiogenic activity of ethanol extract of Bojungbangam-tang, a new herbal prescription composed of nine crude drugs, was evaluated in human umbilical vein endothelial cells (HUVECs). HPLC profile revealed that five major compunds such as apioliquiritin, narirutin, hesperidin, liquiritin and glycyrrhizin. Ethanol extract of Bojungbangam-tang (EBJT) did not showed any significant cytotoxicity against HUVECs up to 200 ug/ml. EBJT significantly inhibited basic fibroblast growth factor (bFGF)-induced HUVECs proliferation to 69% at 200 ${\mu}g/ml$. Migration using window scraping method and tube formation in bFGF stimulated HUVECs were also significantly suppressed by EBJT in a dose-dependent manner. Taken together, these results suggest that Bojungbangam-tang can be a potent prescription for angiogenesis related disease.

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