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A Morphological Study on the Macrophages During Luteolysis in the Pig

돼지 황체에서 황체용해와 대식세포와의 관계

  • 김원식 (충남대학교 의과대학 해부학교실) ;
  • 한승로 (충남대학교 의과대학 해부학교실) ;
  • 손성경 (충남대학교 의과대학 산부인과학교실) ;
  • 박창식 (충남대학교 형질전환복제돼지연구센터) ;
  • 양윤석 (을지대학교 의과대학 산부인과학교실)
  • Published : 2006.04.30

Abstract

In addition to the removal of dying or dead lutein cells by phagocytosis in many species, macrophages exert both luteotropic effect during maturation period and luteolytic effect during degenerative period via mediating autocrine/paracrine actions of self-producing cytokines in the corpus luteum. In this experiment, immunohistochemical and transmission electron microscopic (TEM) studies were performed to observe the morphologic changes of luteal macrophages during luteolysis. A small number of macrophages and low immunoreactivity were present at the mature stage. The number of macrophages and immunoreactivity gradually increased along the advance of luteolysis. Two subtypes of macrophages could be observed through TEM observation. One type of macrophage located between the large lutein cells contained no lipid droplets in their cytoplasm at mature stage. The other type of macrophage located near the blood vessels contained many lipid droplets in their cytoplasm during luteolysis. Particularly, no phagocytic macrophages were observed, which suggested the macrophages in the porcine corpus luteum did not involve in the phagocytotic elimination of dying lutein cells.

Keywords

Corpus luteum;Luteolysis;Macrophage;Immunohistochemistry;TEM

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