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Interpretation of Protein Feed Degradation Pattern in Ruminant Using an Omasal Digesta Sampling Technique

제 3위 소화액 채취기법을 이용한 반추위 단백질 사료 분해 패턴 측정법의 고찰

  • 최창원 (농촌진흥청 축산연구소) ;
  • 백경훈 (농촌진흥청 축산연구소) ;
  • 강수원 (농촌진흥청 축산연구소) ;
  • 이병석 (농촌진흥청 축산연구소) ;
  • 오영균 (농촌진흥청 축산연구소) ;
  • 김경훈 (농촌진흥청 축산연구소)
  • Published : 2006.08.31

Abstract

Present review is to introduce an omasal sampling technique in rumen proteolysis and to consider some information on the omasal sampling technique with particular emphasis on methodological aspects. Use of the omasal sampling technique provides a new opportunity for accurate estimation of rumen metabolism with overcoming limitations of previous in vivo, in vitro and/or in situ methods. The potential advantages of the present technique compared with post-ruminal sampling techniques include following points; 1) only rumen cannulated animals are required, 2) less endogenous nitrogen (N) is contaminated in omasal digesta and 3) omasal digesta are devoid of exposure to acid peptide hydrolysis occurring in the abomasum. Estimates of soluble non-ammonia N (SNAN) in omasal digesta indicate that the assumptions underlying the in situ method that rapidly degradable N fraction can be degraded at an infinite rate and only insoluble dietary N escapes the rumen may be not valid. Quatitatively higher peptide concentration rather than free amino acid and soluble protein in escapable SNAN suggests that hydrolysis of peptide to amino acid may be the rate-limiting step in rumen proteolysis.

Keywords

Omasal sampling technique;Proteolysis;Soluble non-ammonia nitrogen

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