Interpretation of Protein Feed Degradation Pattern in Ruminant Using an Omasal Digesta Sampling Technique

제 3위 소화액 채취기법을 이용한 반추위 단백질 사료 분해 패턴 측정법의 고찰

  • 최창원 (농촌진흥청 축산연구소) ;
  • 백경훈 (농촌진흥청 축산연구소) ;
  • 강수원 (농촌진흥청 축산연구소) ;
  • 이병석 (농촌진흥청 축산연구소) ;
  • 오영균 (농촌진흥청 축산연구소) ;
  • 김경훈 (농촌진흥청 축산연구소)
  • Published : 2006.08.31


Present review is to introduce an omasal sampling technique in rumen proteolysis and to consider some information on the omasal sampling technique with particular emphasis on methodological aspects. Use of the omasal sampling technique provides a new opportunity for accurate estimation of rumen metabolism with overcoming limitations of previous in vivo, in vitro and/or in situ methods. The potential advantages of the present technique compared with post-ruminal sampling techniques include following points; 1) only rumen cannulated animals are required, 2) less endogenous nitrogen (N) is contaminated in omasal digesta and 3) omasal digesta are devoid of exposure to acid peptide hydrolysis occurring in the abomasum. Estimates of soluble non-ammonia N (SNAN) in omasal digesta indicate that the assumptions underlying the in situ method that rapidly degradable N fraction can be degraded at an infinite rate and only insoluble dietary N escapes the rumen may be not valid. Quatitatively higher peptide concentration rather than free amino acid and soluble protein in escapable SNAN suggests that hydrolysis of peptide to amino acid may be the rate-limiting step in rumen proteolysis.


Omasal sampling technique;Proteolysis;Soluble non-ammonia nitrogen


  1. Broderick, G. A 1987. Determination of protein degradation rates using a rumen in vitro system containing inhibitors of microbial nitrogen metabolism. Br. J. Nutr. 58:463-475
  2. Broderick, G. A and Merchen, N. R 1992. Markers for quantifying microbial protein synthesis in the rumen. J. Dairy Sci. 75:2618-2632
  3. Broderick, G. A, Wallace, R. J. and Orskov, E. R. 1991. Control of rate and extent of protein degradation. In Physiological Aspects of Digestion and Metabolism in Rumninants. Tsuda, T., Sasaki, Y. and Kawashima, R.(Eds.) Academic Press, Tokyo, Japan. pp. 541-592
  4. Chen, G., Russell, J. B. and Sniffen, C. J. 1987a. A procedure for measuring peptides in rumen fluid and evidence that peptide uptake can be a rate-limiting step in ruminal protein degradation. J. Dairy Sci. 70, 1211-1219
  5. Chen, Go, Sniffen, C. J. and Russell. J. B. 1987b. Concentration and estimated flow of peptides from the rumen of dairy cattle. Effects of protein quantity, protein solubility, and feeding frequency. J. Dairy. Sci. 70: 983-992
  6. Choi, C. W. 2002. Assessment of the flow of soluble dietary non-ammonia nitrogen escaping degradation in the rumen of dairy cows fed grass silage based diets. Ph.D. Dissertation, Deparment of Animal Science Publications No. 66. University of Helsinki. ISBN 952-10-0855-5(nid.). 60 p. + 4 publ
  7. Choi, C. W., Ahvenjarvi, S., Vanhatalo, A., Toivonen, V. and Huhtanen, P. 2002a. Quantitation of the flow of soluble non-ammonia nitrogen entering the omasal canal of dairy cows fed grass silage based diets. Anim. Feed Sci. Technol. 96:203-220
  8. Choi, C. W., Vauhatalo, A. and Huhtanen, P. 2002c. Concentration and estimated flow of soluble non-ammonia nitrogen entering the omasum of dairy cows as influenced by different protein supplements. Agric. Food Sci. Finl. 11:79-91
  9. Dhanoa, M. S., France, J., Lopez, S., Dijkstra, J., Lister, S. J., Davies, D. R. and Bannink, A. 1999. Correcting the calculation of extent of degradation to account for particulate matter loss at zero time when applying the polyester bag method. J. Anim. Sci. 77:3385-3391
  10. Firkins, J. L., Berger, L. L., Merchen, N. R., Fahey Jr. G. C. and Mulvaney, R. L. 1987. Ruminal nitrogen metabolism in steers as affected by feed intake and dietary urea concentration. J. Dairy Sci. 70:2302-2311
  11. France, J. and Siddons, R. C. 1986. Determination of digesta flow by continuous marker infusion. J. Theor. Biol. 121: 105-119
  12. Hristov, A. and Broderick, G. A. 1994. In vitro determination of ruminal protein degradability using [^{15}N] ammonia to correct for microbial nitrogen uptake. J. Anim. Sci. 72: 1344-1354
  13. Huhtanen, P., Brotz, P. G. and Satter, L. D. 1997. Omasal sampling technique for assessing fermentative digestion in the forestomach of dairy cows. J. Anim. Sci. 75:1380-1392
  14. Huhtancn, P., Vanhatalo, A. and Varvikko, T. 1998. Enzyme activities of rumen particles and feed samples incubated in situ with differing types of cloth. Br. J. Nutr. 79:161-168
  15. Licitra. G.. Hernandez. T. M. and Van Soest, P. J 1996. Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim. Feed Sci. Technol. 57:347-358
  16. Nocek, J. E. 1988. In situ and other methods to estimate ruminal protein and energy digestibility: a review J. Dairy Sci. 71:2051-2069
  17. Nolan. J. V. 1993. Nitrogen Kinetics. In Quantitative aspects of ruminant digetion and metabolism. J. M. Forbes and J. France (Eds.) CAB International Wallingford. Oxon, UK. pp. 123-141
  18. NRC. 2001. National Research Council. Nutrient Requirement of Dairy Cattle. 7th rev. ed. National Acadamy Press. Washington. D.C. USA
  19. Nsereko, V. I., Rooke. J. A.. Newbold. C. J. and Wallace. R. J. 1998. Influence of protease inhibitors on nitrogen distribution In ensiled perennial ryegrass and utilisation of silage nitrogen for growth by rumen bacteria in vitro. Anim. Feed Sci. Technol. 76:51-63
  20. Orskov. E. R. and McDonald. P. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci.. Camb. 92: 499-503
  21. Orskov, E. R.. Macleod. N. A. and Kyle. D. J. 1986. Flow of nitrogen from the rumen and abomasum in cattle and sheep given protein-free nutrients by intragastric infusion. Br. J. Nutr. 56: 241-248
  22. Robinson. P. H. and McQueen. R. E. 1994. Influence of supplemental protein source and feeding frequency on rumen fermentation and performance in dairy cows. J. Dairy Sci. 77: 1340-1353
  23. Robinson. P. H.. Veira, D. M. and Ivan, M. 1998. Influence of supplemental protein quality on rumen fermentation, rumen microbial yield, forestomach digestion. and intestinal amino acid flow in late lactation Holstein cows. Can. J. Anim. Sci. 78: 95-105
  24. Siddons. R. C. Beever, D. E. and Nolan, J. V. 1982. A comparison of methods for the estimation of microbial nitrogen in duodenal digesta of sheep. Br. J. Nutr. 48:377-389
  25. Sniffen. C. J., O'Connor, J. D., Van Soest, P. J.. Fox. D. G. and Russell. J. B. 1992. A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. J. Anim. Sci. 70:3562-3577
  26. Uden, P. 2000. Ruminal metabolism of buffer-soluble proteins. peptides and amino acids in vitro. In Modelling Nutrient Utilization in Farm Animals. J.P. McNamara. J. France and D.E. Beever. (Eds.) CABI Publishing, Oxon, UK. pp. 63-71
  27. Van Straalen, W. M. and Tamminga, S. 1990. Protein degradation of ruminant diets. In Feedstuff evaluation. I. Livestock. Feedingstuffs. Composition. J. Wiseman and D.J.A. Cole. (Eds.) Butterworths. London. pp. 55-72
  28. Varvikko. T. and Lindberg. J. E. 1985. Estimation of microbial nitrogen in nylon-bag residues by feed $^{15}N$ dilution. Br. J. Nutr. 54:473-481
  29. Volden. H.. Mydland. L. T. and Olaisen, V. 2002. Apparent ruminal degradation and rumen escape of soluble nitrogen fractions in grass silage administered intraruminally to lactating dairy cows. J. Anim. Sci. 80:2704-2716
  30. Wallace. R. J. 1991. Rumen proteolysis and its control. In Rumen microbial metabolism and ruminant digestion. J. P. Jouany (Ed.) INRA Editions. Paris. pp. 131-150
  31. Wright. D. E. and Hungate, R. E. 1967. Amino acid concentrations in rumen fluid. Appl. Microbiol. 15: 148-151
  32. Hristov, A. and Broderick, G. A. 1996. Synthesis of microbial protein in ruminally cannulated cows fed alfalfa silage, alfalfa hay or com silage. J. Dairy Sci. 79:1627-1637
  33. Luchini. N. D.. Broderick. G. A. and Combs. D. K. 1996. Characterization of the proteolytic activity of commercial proteases and strained ruminal fluid. J. Anim Sci. 74:685-692
  34. Volden. H.. Velle. W.. Harstad. O. M.. Aulie. A. and Sjaastad. O. V. 1998. Apparent ruminal degradation and rumen escape of lysine, methioinc, and theronine administered intraruminally in mixtures to high-yielding cows. J. Anim. Sci. 76: 1232-1240
  35. Michalct-Dorcau, B. and Ould-Bah. M. Y. 1992. In vitro and in sacco methods for the estimation of dietary nitrogen degradability in the rumen: a review. Anim. Feed Sci. Technol. 40:57-86
  36. Volden. H. and Harstad. O. M. 1995. Effect of rumen incubation on the true indigestibility of feed protein in the digestive tract determined by nylon bag techniques. Acta Agric. Scand., Sect A. Animal Sci. 45:106-115
  37. Lindberg. J. E. 1985. Estimation of rumen degradability of feed proteins with the in sacco technique and various in vitro methods: a review. Acta Agric. Scand.. 25 (Suppl). 64-97
  38. Rosen. H. 1957. A modified ninhydrin colorimetric analysis for amino acids. Arch. Biochem. Biophys. 67: 10-15
  39. Choi, C. W., Vanhatalo, A., Ahvenjarvi, S. and Huhtanen, P. 2002b. Effects of several protein supplements on flow of soluble non-ammonia nitrogen from the forestomach and milk production in dairy cows. Anim. Feed Sci. Technol. 102: 1533
  40. Choi, C. W., Vanhatalo, A. and Huhtanen, P. 2003. Effects of type of grass silage and level of concentrate on the flow of soluble non-ammonia nitrogen entering the omasum of dairy cows. J. Anim. Feed. Sci. 12:3-22
  41. Crooker, B. A., Sniffen, C. J., Hoover, W. H. and Johnson, L. L. 1978. Solvents for soluble nitrogen measurements. J. Dairy Sci. 61 :437-447
  42. Uden. P.. Colucci. P. E. and Van Soest, P. J. 1980. Investigation of chromium. cerium and cobalt as markers in digesta. Rate of passage studies. J. Sci. Food Agric. 31 :625-632
  43. Ahvenjarvi, S., Vanhatalo, A., Huhtanen, P. and Varvikko, T. 1999. Effects of supplementation of a grass silage and barley diet with urea, rapeseed meal and heat-moisture-treated rapeseed cake on omasal digesta flow and milk production in lactating dairy cows. Acta Agric. Scand., Sect. A. Animal Sci. 49: 179-189
  44. Mahadevan. S.. Erfle. J. D. and Sauer. F. D. 1987. Preparation of protease from mixed rumen microorganismsand its use for the III vitro determination of the degradability of true protein in feedstuffs. Can. J. Anim. Sci. 67:55-64