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Novel Mutation and Genetic Variation of the KIT Gene in Korean Wild Boars(Sus scrofa coreanus)

한반도 멧돼지 KIT 유전자의 유전적 변이와 신규 돌연변이

  • Jo, In-Cheol (National Institute of Subtropical Agriculture, RDA) ;
  • Choe, Yu-Rim (National Institute of Subtropical Agriculture, RDA) ;
  • Go, Mun-Seok (National Institute of Subtropical Agriculture, RDA) ;
  • Kim, Jae-Hwan (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Jeong-Gyu (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Hang (Conservation Genome Research Bank for Korean Wildlife, College of Veterinary Medicine, Seoul National University) ;
  • O, Mun-Yu (Department of Life Science, Cheju National University) ;
  • Han, Sang-Hyeon (National Institute of Subtropical Agriculture, RDA)
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 최유림 (농촌진흥청 난지농업연구소) ;
  • 고문석 (농촌진흥청 난지농업연구소) ;
  • 김재환 (경상대학교 응용생명과학부) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부) ;
  • 이항 (서울대학교 수의대학 야생동물유전자원은행) ;
  • 오문유 (제주대학교 생명과학과) ;
  • 한상현 (농촌진흥청 난지농업연구소)
  • Published : 2006.02.28

Abstract

KIT encodes a mast/stem cell growth factor receptor and is known as a possible candidate gene responsible for dominant white coat color in mammals. To investigate the genetic variation of KIT gene in Korean wild boars(Sus scrofa coreanus), we carried out PCR-RFLP and DNA sequencing for three exons(exons 17, 19, and 20) and intron 19 of the KIT gene in Korean wild boars. PCR-RFLP results using NlaⅢ restriction enzyme in the breakpoint region between exon 17 and intron 17 and AciⅠ restriction enzyme in exon 19 indicate that Korean wild boars did not have previously identified white coat color related splicing mutation and missense mutation, respectively. These results also indicate matings between Korean wild boars could not give white coat color offsprings. We also found new SNPs in exons 19(C2661T) and 20(A2760G). Of these, the SNP in exon 20 is a missense mutation which might induce the change of amino acid iso-leucine to valine. However, no relationship was identified with this missense mutation and coat color. In this study, breed specific new SNPs were identified in exons 19, 20 and intron 19 and these results will give important information for genetic variation of porcine KIT gene.

Keywords

Coat color;KIT;Mutation;Pig;RFLP;Wild boar

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