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Development of In vitro Fertilized Bovine Embryos in Medium Supplemented with Matrigel

Matrigel이 첨가된 배양액에서 소 체외수정란의 발달

  • 김동훈 (축산연구소) ;
  • 김세웅 (축산연구소) ;
  • 이민정 (축산연구소) ;
  • 황인선 (축산연구소) ;
  • 배성훈 (축산연구소) ;
  • 양병철 (축산연구소) ;
  • 임기순 (축산연구소) ;
  • 성환후 (축산연구소) ;
  • 양보석 (축산연구소)
  • Published : 2006.12.31

Abstract

The purpose of this study was to investigate the effect of Matrigel on the development of bovine embryos after in vitro fertilization. Bovine embryos were cultured in Ⅰ) SOF+ 0.8% BSA(SOF-B), Ⅱ) SOF+ 0.8% BSA plus 0.8% Matrigel(SOF-M) and III) SOF+0.8% BSA and 10% FBS(SOF-BF). The addition of Matrigel appeared not to increase the proportion of blastocysts (SOF-B, 26.6%; SOF-M, 28.2%; SOF-BF, 26.2%). However, the proportion of hatched blastocysts were significantly increased(P<0.05) by Matrigel(SOF-B, 23.7%; SOF-M, 48.7%; SOF-BF, 18.5%). The means of cell number blastocyst was not significantly different among the treatment groups(SOF-B, 172.7±35.5; SOF-M, 175.1±37.4; SOF-BF, 172.8±38.1). The proportion of apoptotic cells in blastocyst was also found to be not significant among the treatment groups(SOF-B, 3.6±3.2%; SOF-M, 4.3±2.6%; SOF-BF, 4.9±4.3%). In this experiment, Matrigel appeared to support embryonic hatching of bovine embryos. Results suggest that Matrigel, as extracellular matrix components, may be another avenue for formulating more physiological culture system in serum-free culture.

Keywords

Matrigel;Bovine;In vitro fertilization;Hatched blastocyst

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