Inhibition Activity Against Pathogenic Organism of Probiotic Bacteria and Characterization of Inhibition Activity of Isolated Bacteria from Calf Dejecta

Probiotic균주의 Pathogenic Organism에 대한 억제 활성과 송아지분변 분리균주의 억제활성 특성

  • 배임희 (중앙대학교) ;
  • 변정열 (중앙대학교) ;
  • 배귀석 (중앙대학교) ;
  • 이상석 (국립순천대학교) ;
  • 장문백 (중앙대학교) ;
  • 윤영호 (중앙대학교)
  • Published : 2006.12.31


This study was conducted to investigate the inhibitory activity of Lactobacillus spp., Bacillus ssp., and calf fecal isolates against pathogenic Salmonella typhimurium, E. coli, Listeria monocytogenes, and Staphylococcus aureus. Among thirteen strains of Lactobacillus ssp. tested, Lactobacillus helveticus CU631 showed the highest inhibition against three pathogens, whereas Bacillus spp. showed a weak inhibitory activity. Four calf fecal isolates were identified as Lactobacillus pentosus CU13, CU05, Pediococcus pentosaceus CUR02, and Lactobacillus lactis ssp. lactis CUM14. The whole cell and cell wall components of L. rhamnosus CU02 and L. pentosus CU13 were active in the inhibition of L. monocytogenes. The medium components and levels, which affect on the inhibitory activity, were revealed as Tween 80 1.0%, peptone 3.0%, yeast extract 3.0%, glucose 3.0%, beef extract 3.0%, and NaCl 1.0~3.0%, respectively. Inhibitory activity of the supernatant culture medium was not affected by catalase and proteinase K treatment but affected by heat treatment at 80℃ and netralization, which implies that the inhibitory activity is due to the production of organic acids during the growth. L. pentosus CU13 and L. rhamnosus CU02 exhibited broad inhibition spectrum against 16 out of 21 strains including some pathogens. Oral administration of L. rhamnosus CU02 to the mice infected with E. coli O157:H7 was proven to be effective to recover their body weight during the experimental period.


Inhibition activity Lactobacillus spp.;Bacillus spp.;calf dejecta isolates; Escherichia coli O157:H7


  1. Barrow, P. A., Fuller, R. and Newport, M. J. 1977. Changes in the microflora and physiology of the anterior tract of pigs weaned at 2 days, with special reference to the pathogenesis of diarrhea. Infect. Immun. 18:586-595
  2. Biswas, S. R., Ray, P., Johnson, M. C. and Ray, B. 1991. Influence of growth conditions on the production of a bacteriocin, pediocin ACH, by Pediococcus acidilactic H. Appl. Environ. Microbiol. 57:1265-1267
  3. Fuller, R. 1992. Probiotics: The scientific basis. champman and Hall. p.1-3
  4. Ogunbanwo, S. T., Sanni, A. I. and Onilude, A. A. 2003. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis. Afri. J. of Biotechnol. 2(8):219-227
  5. Ogunbanwo, S. T., Sanni, A. I. and Onilude, A. A. 2003. Influence of cultural conditions on the production of bacteriocin by Lactobacillus brevis OG1. Afri. J. Biotechnol. 2(7):179-184
  6. Piard, J. C., Delorme, F., Giraffa, G., Commissaire, J. and Desmazeaud, M. 1990. Evidence for a bacteriocin produced by Lactococcus lactis CNRZ 481. J. Neth. Milk Dairy. 44:143-158
  7. Raibaud, P., Caulet, M. and Galpin, J. V. 1961. Studies on the bacterial flora of the alimentary tract of pigs. II. Streptococci: selective enumer- ation and differentiation of the dominant group. J. Appl. Bacteriol. 24:285-306
  8. Robert, I. L., Michael, R., Sylvia, S. S., Harwig, L., Richard, J. and Patricia, E. 1991. Ultrasensitive assay for endogenous antimicrobial polypeptides. J. Immunologycal Method, 137:167-173
  9. Robinson, I. M., Stromley, H. M. and Varel, V. H. 1988. Streptococcus intestinalis, a new species from the colons and feces of pigs. Int. J. Syst. Bacteriol. 38:245-248
  10. Robinson, I. M., Whipp, S. C. and Bucklin, J. A. 1984. Characterization of predominant bacteria from the colons of normal and dysenteric pigs. Appl. Environ. Microbiol. 24:285-306
  11. Schillinger, U. and Lucke, F. K. 1989. Antimicrobial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55:1901-1906
  12. Steel, R. G. D. and Torrie, J. H. 1981. Principles and procedures of statistics, 2nd ed. McGraw-Hill, New York
  13. Venkitanarayanan, K. S., Lin, C.-M., Bailey, J. and Doyle, M. P. 2002. Inactivation of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes on apples, oranges, and tomatoes by lactic acid with hydrogen peroxide. J. Food Protection. 65(1):100-105
  14. Zani, G., Biavati, B. and Crociani, F. 1974. Bifidobacteria from the feces of piglets. J. Appl. Bacteriol. 3:537-547
  15. Jonsson, E. and Conway, P. 1992. Probiotics for pigs. In Probiotics- the science basis, ed R. Fuller, Champman and Hall, 260-316
  16. Axelsson, L. and Lindgren, S. 1987. Characterization and DNA homology of Lactobacillus strains isolated from pig interstine. J. Appl. Bacteriol. 62:433-440
  17. Joerger, M. C. and Klaenhammer, T. R. 1986. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J. Bacteriol. 167:439-446