Association Between the Polymorphism in the Promoter Region of Porcine A-FABP Gene and Growth Traits in Duroc Pigs

돼지 Duroc 품종에서 A-FABP promoter의 다형성과 성장형질의 연관성

  • Han, Sang-Hyeon (National Institute of Subtropical Agriculture, RDA) ;
  • Jo, In-Cheol (National Institute of Subtropical Agriculture, RDA) ;
  • Lee, Jong-Eon (National Institute of Subtropical Agriculture, RDA) ;
  • Kim, Hyo-Seon (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Jeong-Gyu ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • O, Mun-Yu (Department of Life Science, Cheju National University) ;
  • Go, Mun-Seok (National Institute of Subtropical Agriculture, RDA)
  • 한상현 (농촌진흥청 난지농업연구소) ;
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 이종언 (농촌진흥청 난지농업연구소) ;
  • 김효선 (경상대학교 응용생명과학부) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부) ;
  • 오문유 (제주대학교 생명과학과) ;
  • 고문석 (농촌진흥청 난지농업연구소)
  • Published : 2006.02.28


A polymorphism was found in the promoter region of porcine adipocyte fatty acid binding protein gene(A-FABP) gene which plays a key role in the binding and transportation of free fatty acid in adipocyte and deposition of intramuscular fat. Mutation was detected a substitution(T406C) using SSCP analysis and subsequently confirmed by sequencing the fragment in Duroc pigs. This T-406C mutation might change the binding activity for transcription factor nuclear factor 1(NF1). In this population, this mutation was genotyped using HinfⅠRFLP, and found three kinds of genotypes(TT, TC, and CC) showing their frequencies of 42.3, 44.3, and 13.4%, respectively. We statistically analyzed the association between the A-FABP genotypes and growth traits and found that the body weights of the pigs containing 406C/(TC or CC) were heavier for the body weight at the age of 20 weeks than those containing genotype TT(P<0.05), but not for those at the age of 0, 3, and 10 weeks. Pigs containing genotype CC had also a higher value for the average daily gain and lower values for the date for 90kg of body weight and food conversion ratio than those of 406T/- genotype. In addition, without the significant difference of back fat thickness, there was a significant association between the existence of allele CC and lean meat and eye muscle area(P<0.05). As a result of this study, we suggest that the allele T406C in the promoter region of A-FABP gene play an important role in deposition of intramuscular fat and weight in the later growth period. This polymorphism will be an useful molecular marker for breeding of Duroc pigs.


A-FABP;Association;Duroc;Growth trait;Pig;Polymorphism


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