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Association Between the Polymorphism in the Promoter Region of Porcine A-FABP Gene and Growth Traits in Duroc Pigs

돼지 Duroc 품종에서 A-FABP promoter의 다형성과 성장형질의 연관성

  • Han, Sang-Hyeon (National Institute of Subtropical Agriculture, RDA) ;
  • Jo, In-Cheol (National Institute of Subtropical Agriculture, RDA) ;
  • Lee, Jong-Eon (National Institute of Subtropical Agriculture, RDA) ;
  • Kim, Hyo-Seon (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Jeong-Gyu ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • O, Mun-Yu (Department of Life Science, Cheju National University) ;
  • Go, Mun-Seok (National Institute of Subtropical Agriculture, RDA)
  • 한상현 (농촌진흥청 난지농업연구소) ;
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 이종언 (농촌진흥청 난지농업연구소) ;
  • 김효선 (경상대학교 응용생명과학부) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부) ;
  • 오문유 (제주대학교 생명과학과) ;
  • 고문석 (농촌진흥청 난지농업연구소)
  • Published : 2006.02.28

Abstract

A polymorphism was found in the promoter region of porcine adipocyte fatty acid binding protein gene(A-FABP) gene which plays a key role in the binding and transportation of free fatty acid in adipocyte and deposition of intramuscular fat. Mutation was detected a substitution(T406C) using SSCP analysis and subsequently confirmed by sequencing the fragment in Duroc pigs. This T-406C mutation might change the binding activity for transcription factor nuclear factor 1(NF1). In this population, this mutation was genotyped using HinfⅠRFLP, and found three kinds of genotypes(TT, TC, and CC) showing their frequencies of 42.3, 44.3, and 13.4%, respectively. We statistically analyzed the association between the A-FABP genotypes and growth traits and found that the body weights of the pigs containing 406C/(TC or CC) were heavier for the body weight at the age of 20 weeks than those containing genotype TT(P<0.05), but not for those at the age of 0, 3, and 10 weeks. Pigs containing genotype CC had also a higher value for the average daily gain and lower values for the date for 90kg of body weight and food conversion ratio than those of 406T/- genotype. In addition, without the significant difference of back fat thickness, there was a significant association between the existence of allele CC and lean meat and eye muscle area(P<0.05). As a result of this study, we suggest that the allele T406C in the promoter region of A-FABP gene play an important role in deposition of intramuscular fat and weight in the later growth period. This polymorphism will be an useful molecular marker for breeding of Duroc pigs.

Keywords

A-FABP;Association;Duroc;Growth trait;Pig;Polymorphism

References

  1. Andersson, L., Haley, C. S., Ellergren, H., Knott, S. A., Johansson, M., Andersson, K., Andersson-Eklund, L., Edfor-Lilja, I., Fredholm, M., Hansson, I., Hakansson, J. and Lundstrom, K. 1994. Genetic mapping of quantitative loci for growth and fatness in pigs. Science 263:1771-1774 https://doi.org/10.1126/science.8134840
  2. Armstrong, M. K., Bernlohr, D. A., Storch, J. and Clarke. S. D. 1990. The purification and characterization of a fatty acid binding protein specific to pig (Sus domesticus) adipose tissue. Biochem. J. 267:373-378 https://doi.org/10.1042/bj2670373
  3. Buskin. J. N. and Hauschka, S. D. 1989. Identification of a myocyte nuclear factor that binds to the muscle-specific enhancer of the mouse muscle creatine kinase gene. Mol. Cell. Biol. 9:2627-2640 https://doi.org/10.1128/MCB.9.6.2627
  4. Cooke. D. W. and Lane, M. D. 1999. Transcription factor NF1 mediates repression of the GLUT4 promoter by cyclic-AMP. Biochem. Biophys. Res. Commun. 260:600-604 https://doi.org/10.1006/bbrc.1999.0959
  5. Degterev. A. and Foster, J. A. 1999. The role of NF-1 factors in regulation of elastin gene transcription. Matrix Biol. 18:295-307 https://doi.org/10.1016/S0945-053X(99)00020-7
  6. De Vol. D. L. E., McKeith, F. K., Bechtel, P. J., Novakofski, J., Shanks, R. D. and Carr, T. R. 1988. Variation in composition and palatability traits and relationships between muscle characteristics and palatability in a random sample of pork carcasses. J. Anim. Sci. 66:385-395 https://doi.org/10.2527/jas1988.662385x
  7. Dowell, P. and Cooke, D. W. 2002. Olf-1/early B cell factor is a regulator of glut4 gene expression in 3T3-L1 adipocytes. J. Biol. Chem. 277:1712-1728 https://doi.org/10.1074/jbc.M108589200
  8. Furlong, E. E., Rein, T. and Martin, F. 1996. YY1 and NF1 both activate the human p53 promoter by alternatively binding to a composite element. and YY1 and E1A cooperate to amplify p53 promoter activity. Mol. Cell. Biol. 16:5933-5945 https://doi.org/10.1128/MCB.16.10.5933
  9. Gerbens, F., de Koning, D. J., Harders, F. L., Meuwissen, T. H., Janss, L. L., Groenen, M. A., Veerkamp, J. H., van Arendonk, J. A. and te Pas, M. F. 2000. The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs. J. Anim. Sci. 78:552-559
  10. Gerbens. F., van Erp, A. J., Harders. F. L., Verburg, F. J., Meuwissen, T. H., Veerkamp. J. H. and te Pas. M. F. 1999. Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. J. Anim. Sci. 77:846-852
  11. Gerbens, F., Verburg, F. J., van Moerkerk, H. T., Engel, B., Buist, W., Veerkamp, J. H. and te Pas, M. F. 2001. Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs. J. Anim. Sci. 79:347-354
  12. Jones, K. A., Kadonaga, J. T., Rosenfeld. P. J., Kelly, T. J. and Tjian, R. 1987. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication. Cell 48:79-89 https://doi.org/10.1016/0092-8674(87)90358-8
  13. Lin, W. H., Huang, L. S., Ren, J., Deng, S. H., Wang, W. J., Liu, B. S., Zhou, L. H. and Chen, C. Y. 2002. Research on genetic variation of heart fatty acid-binding protein gene in ten pig breeds. Yi Chuan Xue Bao 29:12-15
  14. Marklund. L., Nystrom, P., Stern, S., Andersson-Eklund. L. and Andersson. L. 1999. Confirmed quantitative trait loci for fatness and growth on pig chromosome 4. Heredity 82:134-141 https://doi.org/10.1038/sj.hdy.6884630
  15. Miura, S., Tsunoda, N., Ikeda, S., Kai, Y., Cooke. D. W., Lane. M. D. and Ezaki, O. 2004. Nuclear factor 1 regulates adipose tissue-specific expression in the mouse GLUT4 gene. Biochem. Biophys. Res. Commun. 325:812-818 https://doi.org/10.1016/j.bbrc.2004.10.117
  16. Miura, S., Tsunoda, N., Ikeda. S., Kai, Y., Ono, M., Maruyama, K., Takahashi. M., Mochida, K., Matsuda, J., Lane, M. D. and Ezaki. O. 2003. Regulatory sequence elements of mouse GLUT4 gene expression in adipose tissues. Biochem. Biophys. Res. Commun. 312:277-284 https://doi.org/10.1016/j.bbrc.2003.10.114
  17. Nebl, G. and Cato, A. C. 1995. NF1/X proteins: a class of NF1 family of transcription factors with positive and negative regulatory domains. Cell. Mol. Biol. Res. 41:85-95
  18. Nechtelberger, D., Pires, V., Soolknet, J., Stur, I., Brem, G., Mueller, M. and Mueller. S. 2001. Intramuscular fat content and genetic variants at fatty acid-binding protein loci in Austrian pigs. J. Anim. Sci. 79:2798-2804
  19. Oh, M. Y. and Jung, Y. H. 2001. Mitochondrial DNA polymorphism in Cheju and Tsushima native horses using SSCP analysis. Korean J. Genet. 23:35-43
  20. Ovilo. C., Oliver, A., Noguera, J. L., Clop, A., Barrangan, C., Varona, L., Rodriguez, C., Toro, M., Sanchez, A., Perez-Enciso, M. and Silio, L. 2002. Test for positional candidate genes for body composition on pig chromosome 6. Genet. Sel. Evol. 34:465-479 https://doi.org/10.1186/1297-9686-34-4-465
  21. Pang, W. J., Sun, S. D., Li, Y., Chen, G. D. and Yang, G. S. 2005. Relationship between molecular marker of western main pig H-FABP gene and IMF content. Yi Chuan 27:351-356
  22. Sato, S., Oyamada, Y., Atsuji. K., Nade. T., Sato, S-I., Kobayashi, E., Mistuhashi, T ., Nirasawa, K., Komatsuda, A., Saito, Y., Terai, S., Hayashi, T. and Sugimoto, Y. 2003. Quantitative trait loci analysis for growth and carcass traits in a Meishan$\times$Duroc $F_2$ resource population. J. Anim. Sci. 81:2938-2949
  23. Scheepers, A., Doege, H., Joost, H. G. and Schurmann, A. 2001. Mouse GLUT8: genomic organization and regulation of expression in 3T3-L1 adipocytes by glucose. Biochem. Biophys. Res. Commun. 288:969-974 https://doi.org/10.1006/bbrc.2001.5866
  24. Singh, M. V. and Ntambi, J. M. 1998. Nuclear factor 1 is essential for the expression of stearoyl-CoA desaturase 1 gene during preadipocyte differentiation. Biochim. Biophys. Acta 1398:148-156 https://doi.org/10.1016/S0167-4781(98)00037-2
  25. Urban, T., Mikolasova, R., Kuciel, J., Ernst, M. and Ingr, I. 2002. A study of associations of the H-FABP genotypes with fat and meat production of pigs. J. Appl. Genet. 43:505-509
  26. Veerkamp, J. H. and van Moerkerk, H. T. 1993. Fatty acid-binding protein and its relation to fatty acid oxidation. Mol. Cell Biochem. 123:101-106 https://doi.org/10.1007/BF01076480
  27. Zimmerman, A. W. and Veerkamp, J. H. 1998. Members of the fatty acid-binding protein family inhibit cell-free protein synthesis. FEBS Lett. 437:183-186 https://doi.org/10.1016/S0014-5793(98)01224-1
  28. 김재환, 박응우, 박정진, 최봉환, 김태헌, 서보영, 정일정, 임현태, 오성종, 이정규, 전진태. 2005. 돼지 FASP3 promoter 부위 내 신규 돌연변이 탐색과 근내지방도와의 연관성 분석. 한국동물자원과학회지 47: 1-10
  29. 조인철, 최유림, 고문석, 김효선, 이정규, 전진태, 한상현. 2005. 돼지 Landrace 품종에서 Myostatin 유전자의 유전적 다양성과 성장형질의 연관성. 한국동물자원과학회지 47:159-166
  30. Gerbens, F., Jansen, A., van Erp, A. J., Harders, F., Meuwissen, T. H., Rettenberger. G ., Veerkamp, J. H. and te Pas, M. F. 1998. The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs. Mamm. Genome 9: 1022-1026 https://doi.org/10.1007/s003359900918
  31. Karsenty, G., Golumbek, P. and de Crombrugghe, B. 1988. Point mutations and small substitution mutations in three different upstream elements inhibit the activity of the mouse alpha 2(I) collagen promoter. J. Biol. Chem. 263:13909-13915
  32. Gao, B. and Kunos, G. 1998. Cell type-specific transcriptional activation and suppression of the $\alpha_{1\beta}$ adrenergic receptor gene middle promoter by nuclear factor 1. J. Biol. Chem. 273:31784-31787 https://doi.org/10.1074/jbc.273.48.31784
  33. Rein, T., Foster, R., Krause, A., Winnacker, E.-L. and Zorbas, H. 1995. Organization of the alphaglobin promoter and possible role of nuclear factor I in an alpha-globin-inducible and a noninducible cell line. J. Biol. Chem. 270:19643-19650 https://doi.org/10.1074/jbc.270.33.19643
  34. Jahroudi, N., Ardekani, A. M. and Greenberger, J. S. 1996. An NF1-like protein functions as a repressor of the von Willebrand factor promoter. J. Biol. Chem. 271:21413-21421 https://doi.org/10.1074/jbc.271.1.21
  35. Graves, R. A., Tontonoz, P., Ross, S. R. and Spiegelman, B. M. 1991. Identification of a potent adipocyte-specific enhancer: involvement of an NF-1-like factor. Genes Dev. 5:428-437 https://doi.org/10.1101/gad.5.3.428
  36. Nakamura, M., Okura, T., Kitami, Y. and Hiwada, K. 2001. Nuclear factor 1 is a negative regulator of gadd153 gene expression in vascular smooth muscle cells. Hypertension 37:419-424 https://doi.org/10.1161/01.HYP.37.2.419

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