Effects of Dietary Conjugated Linoleic Acid Levels and Periods on Meat Quality in Breast and Thigh Muscles of Broiler

Conjugated Linoleic Acid 급여기간과 첨가수준이 저장기간 중 육계의 부위별 품질특성에 미치는 영향

  • 문성실 (충남동물자원센터, 건양대학교) ;
  • 신철우 (경상대학교 동물자원과학부) ;
  • 주선태 (경상대학교 동물자원과학부) ;
  • 박구부 (경상대학교 동물자원과학부)
  • Published : 2006.02.28


A total of 180 Arbor Acre broilers (35 days of age) were fed a diet containing 0%, 0.6%, 1.2%, 1.8%, 2.4% or 4.8% conjugated linoleic acid (CLA) for 3 weeks then slaughtered at week 1, 2 and 3. Thigh and breast muscles were seamed out, vaccum-packed prior to storage in a chill for days 7 to analyse thiobarbituric acid reactive substances (TBARS). The accumulation of CLA in breast and thigh muscles was increased significantly (P<0.05) as the CLA level was increased, but no significant difference amongst the treatments. The contents of saturated fatty acids were significantly increased(P<0.05) with an increase of dietary CLA level, but those of unsaturated fatty acids were significantly decreased (P<0.05). The content of mono unsaturated fatty acid (MUFA) was decreased as dietary CLA level increased, even though that of poly unsaturated fatty acid (PUFA) had a little difference between dietary CLA level. Dietary CLA reduced the degree of lipid oxidation in raw chicken meat during storage.


Conjugated linoleic acid (CLA);Broiler;TBARS;Fatty acid;Lipid oxidation


  1. Belury, M. A. 1995. Conjugated dienoic linoleate: A polyunsaturated fatty acid with unique chemoprotective properties. Nutr. Rev. 53:83-89
  2. Burge, J. A. and Aust. S. D. 1978. Microsomal lipid peroxidation. Methods Enzymol. 52:302-303
  3. Du, M., Nam, K. C., Hur, S. J., Ismail, H. A., Kim. Y. H. and Ahn, D. U. 2001b. Effect of dietary conjugated linoleic acid (CLA) on the growth, fat accumulation and meat quality of broilers. Poul. Sci. In press
  4. Dormandy, T. L. and Wickens, D. G. 1987. The experimental and clinical pathology of diene conjugation. Chem Phys. Lipid. 45:353-361
  5. Folch. L Lees. M. and Sloane-Stanley, G. H. 1957. A simple method for the isolation and purification of total lipid form animal tissues. J. Biol. Chem. 226:497
  6. Du, M., Ahn, D. U. and Sell, J. L. 1999. Effect of dietary CLA on the composition of egg ylok lipids. Poultry Sci. 78:1639-1645
  7. Du, M., Ahn, D. U., Narn, K. C. and Sell. J. L. 2000. Influence of dietary conjugated linoleic acid on volatile profiles, color and lipid oxidation of irradiated raw chicken meat. Meat Sci. 56:387-395
  8. Du. M., Ahn. D. U., Nam. K. C. and Sell. J. L. 2001. Volatile profiles and lipid oxidation of irradiated cooked chicken meat from laying hens fed with diets containing conjugated linoleic acid. Poul. Sci. 80:235-241
  9. Du, M., Nam, K. C. Hur, S. J., Ismail, H. and Ahn. D. U. 2002. Effect of dietary conjugated linoleic acid, irradiation, and packaging conditions on the quality characteristics of raw broiler breast fillets. Meat. Sci. 60:9-15
  10. Nicolosi, R. J., Rogers, E. J., Kritchevsky, D., Scimeca, J. A. and Huth, P. J. 1997. Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery. 22:266-277
  11. Park, Y., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. E. and Pariza, M. W. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids. 32:853-858
  12. SAS. SAS/STAT Software for pc, SAS/STAT user's guide. 1998. Statistics. SAS inst., Cary. NC
  13. Yurawecz. M. P., Hood. J. K., Mossoba M. M., Roach. J. A. and Ku. Y. 1995. Furan fatty acids determined as oxidation products of conjugated octadecadienoic acid. Lipids. 30:595-598
  14. 박구부, 이정일, 하영래, 강석중, 진상근, 주선태. 1999b. 난황내 Conjugated Linoleic Acid(CLA)가 지방산조성과 지방산화에 미치는 효과. 한국축산식품학회 18(4):339-347
  15. Ha, Y. L., Storkson, J. M. and Pariza, M. W. 1990. Inhibition of benzo($\alpha$) pyrone-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50:1097-1101
  16. Ha, Y. L. and Pariza, M. W. 1991. Naturally-occurring novel anti carcinogens : conjugated dienoic derivatives of linoleic acid (CLA). J. Kor. Soc. Food Nutr. 24(4):401-407
  17. Hughes. P. E., Hunter, W. J. and Tove. S. B. 1982. Biohydrogenation of unsaturated fatty acids. Purification and properties of cis. 9-transoctadecadienoate reductase. J. Biol. Chem. 257:3643-3649
  18. Lin, H., Boylston, T. D., Chang, M. J., Luedecke, L. 0., Shultz. T. D. and Boylston, T. D. 1995. Survey of the conjugated linoleic acid contents of dairy products. J. Dairy Sci. 78:2358
  19. Cook, M. E., Miller, C. C., Park. Y. and Pariza, M. 1993. Immune modulation by altered nutrient metabolism: Nutritional control of immune-induced growth depression. Poultry Sci. 72:1301-1305
  20. Chin, S. F., Liu, W., Storkson. J. M., Ha., Y. L. and Pariza, M. W. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid. a newly recognized class of anti carcinogens. J. Food Comp. Anal. 5:185-197
  21. Stangl. G. I. 2000. High dietary levels of a conjugated linoleic acid mixture alter hepatic glycerophospholipid class profile and cholesterol-carrying serum lipoproteins of rats. J. Nutr. Bioche. 11: 184-191
  22. Ha, Y. L., Grimm, N. K. and Pariza, M. W. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8:1881-1887
  23. Ha, Y. L. and Kim, J. O. 1994. Culture tube method for the determination of total cholesterol in egg yolk lipid. J. Korean Soc. Food Nutr., 23:1032
  24. Chin, S. F., Storkson, J. M., Albright. K. J., Cook, M. E. and Pariza, M. W. 1994. Conjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. J. Nutr. 124:2344-2349
  25. 박구부, 이정일, 박태선, 김진형, 신택순, 강석중, 하영래, 주선태. 1999. CLA 급여가 난황의 콜레스테롤과 CLA 함량에 미치는 효과. 한국축산학회지. 41:65
  26. Miller. C. C., Park, Y., Pariza, M. W. and Cook, M. E. 1994. Feeding conjugated linoleic acid to animals partially overcomes catabolic response due to endotoxin injection. Biochem. Biophys. Res. Commun. 198:1107-1112
  27. 이정일, 주선태, 최병대, 하영래, 하정기, 박구부. 1999. Conjugated Linoleic Acid(CLA) 급여기간에 계육의 CLA 함량과 지망산 조성에 미치는 영향. 한국축산학회지 41(3):375-386
  28. Ha, Y. L., Nancy K. Grimm and Michael W. Pariza. 1989. Newly Recognized Anticarcinogenic Fatty acids : Identification and Quantification in Natural and Processed Cheeses. J. Agric. Food Chem. 37:75-81
  29. Ahn, D. U., Sell, J. L., Jo. C., Chamruspollert. M. and Jeffrey, M. 1999. Effect of dietary conjugated linoleic acid on the quality characteristics of chicken eggs during refrigerated storage. Poultry Science. 78(6):922-928
  30. Joo, S. T., Lee. J. 1., Ha. Y. L. and Park. G. B. 2002. Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color and water-holding capacity of pork loin. J. Ani. Sci. 80:108-112