Effects of Alcoholic Feeds on In vitro Ruminal pH, Ammonia, Alcohol and Volatile Fatty Acids Concentrations

Alcohol 사료가 In vitro 반추위내 pH, Ammonia, Alcohol 및 Volatile Fatty Acids 농도에 미치는 영향

  • 신종서 (강원대학교 동물자원학부) ;
  • 박병기 (농촌진흥청 축산연구소)
  • Published : 2006.02.28


In this study, changes of ammonia, alcohol and volatile fatty acid(VFA) concentrations or pH in in vitro ruminal fluid were studied to determine the effects of alcoholic feeds on in vitro ruminal fermentation characteristics. To formulate the alcoholic feeds, alcohol was added to commercial formulated feed at the levels of 1, 3, and 5 %. Experiments were done with four treatment groups, control(commercial feed), AF-1(commercial feed+1% alcohol), AF-3(commercial feed+3% alcohol), and AF-5(commercial feed+5% alcohol). Ammonia concentrations of AF-1 and AF-5 were significantly lower than that of control for the 12h incubation(p<0.05). Ruminal alcohol concentration was increased with the addition level of alcohol increased(p<0.05). TVFA concentrations of AF-1, AF-3 and AF-5 were significantly higher than those of control at 12h(p<0.05). Significant decrease of molar percentage of acetate was observed in control from 8 to 12h incubation, but molar percentage of acetate for AF-1, AF-3 and AF-5 was constant. Molar percentage of propionate was increased in control compared with AF-1, AF-3 and AF-5 from 8 to 12h incubation(p<0.05). Molar percentages of butyrate and valerate were higher in AF-1, AF-3 and AF-5 than in control(p<0.05). Molar percentage of caproate for AF-1, AF-3 and AF-5 was 0.05, 0.58 and 0.47M% at 8h, respectively, but that was not detected for control. Present results may indicate that the alcoholic feeds show positive effects on in vitro ruminal ammonia, alcohol and VFA concentrations or pH. Furthermore, the results of this study implies that the addition level of 5% could be more effective to ruminal fermentation than other addition levels.


Alcoholic feeds;Ammonia;Alcohol;Volatile fatty acid;Hanwoo


  1. 津吉 炯, 深谷幸作, 飯原愼一, 中原信夫, 熱田眞由美, 針生程吉. 1990. 黑毛和種肥肉牛の仕土期におけるアルコ-ル給輿がその肉質に及び肉質への効果影響. 肉用牛硏究會 第28回大會講演要旨, p.51
  2. 板橋久雄, 小林 剛, 松本光人. 1989. 牛のルーメン醱酵と血漿成分に及ぼすエタノール添加の影響. 畜産試驗場硏究報告 第49号
  3. 이은a. 1999. 분말알코올의 급여수준이 농후사료 다급시 한국재래산양의 제 l위내 미생물상에 미치는 영향. 한국낙농학회지. 21:193-200
  4. 이은b. 1999. 분말알코올의 급여수준이 농후사료 다급시 한국 재래산양의 제 l위내 발효 및 효소 활성에 미치는 영향. 한국낙농학회지. 21:201-206
  5. 정유열, 이은. 1996. 조사료 다급시 분말 알코올의 첨가가 한국재래산양의 제 l위내 미생물상과 발효양상에 미치는 영향. 한국낙농학회지. 18:1-6
  6. Fonty, G., Gouet, P., Jouany, J. P. and Senaud, J. 1983. Ecological factors determining establishment of cellulolytic bacteria and protozoa in the rumen of mexenic lambs. J. Gen. Microbiol.129:213-223
  7. Kurihara, Y., Takechi, T. and Shibata F. 1978. Relationship between bacteria and ciliate protozoa in the rumen of sheep fed on a purified of diet. J. Agric. Sci. Amb. 90:373-381
  8. Leng, R. A. 1970. Formation and production of volatile fatty acids in the rumen, in Physiology of digestion and metabolism in the rumen(Phillipson, A. T., ed), Oriel Press, Newcastle upon Tyne. pp. 406-421
  9. Moomaw, R. C. and Hungate, R. E. 1963. Ethanol conversion in the bovine rumen. J. Bacteriol. 85:721-722
  10. SAS. 1999. SAS/STAT Software for PC. Release 6.11, SAS Institute, Cary. NC, U.S.A
  11. Wolin, M. J. and Miller, T. L. 1997. Microbe-Microbe interactions. In The rumen microbial ecosystem. Hobson. P. N. and C. S. Stewart(Eds). Blackoe Academic & Professional. London
  12. 宮崎孔志, 目野常男. 板橋久雄. 1989. ルーメン 微生物の醱酵バターンと細胞膜脂肪酸組成に及ぼすエタノールの影響. 目畜會報. 60:776-782
  13. 大槻和夫, 小林 剛, 松本光人, 板橋久雄. 1991. 濃厚飼料多給牛のルーメンに及ぼすエタノール 添加の影響. 畜産試驗場硏究報 第51卷. pp. 9-13
  14. 牛田出一成. 1998. 反?動物の營養生理學: 第2章 ルーメン微生物の機能と役割, 農文協. pp. 137-149
  15. Alcohol 飼料化硏究推進委員會. 1993. 飼料用 Alcohol
  16. AOAC. 1995. Official Methods of Analysis. 16th Ed. Association of Official Analytical Chemist, Washington D. C., USA
  17. Bryant, M. P. 1956. The characteristics of strains of Selenomonas isolated from bovine rumen contents. J. Bacteriol. 72:162-167
  18. Burning. C. L. and Yokoyama M. T. 1988. Characteristics of live and killed Brewer's yeast slurries and intoxication by intraruminal administration to cattle. J. Anim. Sci. 66:585-591
  19. Czerkawski, J. W. and Breckentidge. G. 1972. Fermentations of various glycolytic intermediates and other compounds by rumen micro-organisms with particular reference to methane production. Br. J. Nutr. 27:131-146
  20. Andree Durix, C., Jean-Blain, H., Sallmann, P. and Jouany, J. P. 1991. Use of a semicontinuous culture system(RUSSITEC) to study the metabolism of ethanol in the rumen and its effects on ruminal digestion. Can. J. Anim. Sci. 71:115-123