DOI QR코드

DOI QR Code

Development of Simultaneous Analysis for the Multi-residual Pesticides in the Ginseng Extract using Gas Chromatography

인삼농축액에서 GC를 이용한 잔류농약 동시다성분 분석법의 개발

  • Shin Yeong-Min (Busan Regional Food&Drug Administration) ;
  • Lee Seon-Hwa (Busan Regional Food&Drug Administration) ;
  • Son Yeong-Uk (Busan Regional Food&Drug Administration) ;
  • Jeong Ji-Yoon (Residue & Chemicals Team, Korea Food & Drug Administration) ;
  • Jeoung Seoung-Wook (School of Environmental Science & Engineering, Inje University) ;
  • Park Heung-Jai (School of Environmental Science & Engineering, Inje University) ;
  • Kim Sung-Hun (School of Culinary arts & Food Management, Youngsan University) ;
  • Won Young-Jun (Busan Regional Food&Drug Administration) ;
  • Lee Chang-Hee (Busan Regional Food&Drug Administration) ;
  • Kim Woo-Seong (Busan Regional Food&Drug Administration) ;
  • Hong Moo-Ki (Residue & Chemicals Team, Korea Food & Drug Administration) ;
  • Chae Kab-Ryong (Busan Regional Food&Drug Administration)
  • 신영민 (부산지방식품의약품안전청) ;
  • 이선화 (부산지방식품의약품안전청) ;
  • 손영욱 (부산지방식품의약품안전청) ;
  • 정지윤 (식품의약품안전청 잔류물질화학팀) ;
  • 정성욱 (인제대학교 환경공학부) ;
  • 박흥재 (인제대학교 환경공학부) ;
  • 김성훈 (영산대학교 동양조리학과) ;
  • 원영준 (부산지방식품의약품안전청) ;
  • 이창희 (부산지방식품의약품안전청) ;
  • 김우성 (부산지방식품의약품안전청) ;
  • 홍무기 (식품의약품안전청 잔류물질화학팀) ;
  • 채갑용 (부산지방식품의약품안전청)
  • Published : 2006.01.01

Abstract

The simultaneous analysis of multi-residual pesticides was developed using a gas chromatography (GC) method. In this study, a simple and reliable methodology was improved to detect 154 kinds of pesticides in sinseng extract sample by using a liquid-liquid extraction procedure, open column chromagraphy and chromatographic analysis by CC electron capture detector (ECD) and GC nitrogen-phosphorus detector (NPD). The 154 kinds of pesticides were classified in 4 groups according to the chemical structure. The extraction of pesticides was experimented with $70\%$ acetone and dichloromethane/petroleum ether in order, and cleaned up via open column chromatography $(3\times30cm)$ packed with florisil $(30g,\;130^{\circ}C,\;12hrs)$. The final extract was concentrated in a rotator evaporator at $40^{\circ}C$ until dryness. Then the residue was redissolved to 2ml with acetone, and analyzed by GC-ECD and GC-NPD. The applied concentration of pesticides was over $1\~10{\mu}g/ml$. The recovery tests were ranged from $70.7\%$ to $115.2\%$ with standard deviations between 0.3 and $5.7\%$ of the standard spiked to the ginseng extract sample (Group $I\~IV$). The limit of detection (LOD) ranged from 0.001 to $0.099{\mu}g/ml$ (Group $I\~IV$). The 9 kinds of pesticides were not detected. The developed method was applied satisfactory to the determination of the 154 kinds of pesticides in the ginseng extract with good reproducibility and accuracy.

Keywords

Ginseng extract;Simultaneous analysis;Multi-residual pesticide;Gas chromatography

References

  1. 이재열, 1996, 인삼과 건강, 한림출판사
  2. 조재성 외, 1998, 인삼재배, 선진출판사
  3. 박창규 외, 1994, 농약의 생화학과 사용법, 신일상사
  4. 잔류농약기준편람, 1994, 일본식품위생협회
  5. Pesticide residues in food - toxicalogical and environmental, 1995, Joint FAO/WHO meet­ing on pesticide residue
  6. 식품공전, 2004, 식품의약품안전청
  7. Dejonckheere, W., W. Steurbaut and S. Srieghe, 1996, Monitoring of pesticide resi­dues in fresh vegetables, fruits, and selected food items in Belgium 1991-1993, J. AOAC International, 79, 97-110
  8. Kim, T. J., S. J. Park and Y. S. Kim, 1985, Studies on the Simultaneous Analysis of Organochlorine Pesticide Residues by Gas­Liquid Chromatography (II). Determination of Pesticides by GLC, J. Korean Chem. Sci., 29(5), 503-509
  9. Kim, T. J., W. E. Yun and Y. S. Kim, 1987, A Study on the Separation and Determination of Organochlorine Pesticides by Capillary Column Gas-liquid Chromatography, J. Korean Chem. Sci., 31(5), 42S-433
  10. Kim, T. J., W. E. Yun and T. S. Rhee, 1991, A Study on the Simultaneous Analysis of Regulated Pesticides Residues from Rice and Soy Bean, J. Korean Chem. Sci., 35(5), 560-­568
  11. Leoni, V., A. M. Caricchia and S. Chiavarini, 1992, J. AOAC International, 75, 511-518
  12. Pang, G. F., Y. Z. Chao and C. L. Fan, 1995, Modification of AOAC multiresidue method for determination of synthetic pyrethroid res­idues in fruits, vesitable, and grains, Part I: Acetonitrile extraction system and opti­mization of florisil cleanup and gas chroma­tography, J. AOAC International., 78, 1481-­1488
  13. Lee, B. H., W. S. Kim, W. W. Park, S. U. Jeong and H. J. Park, 1997, Study on the de­velopment of analytical method (multi-pesti­cide residue method) for the organophosphate pesticides, J. Korean Envi. Sci. Soc., 6(2), 183-187
  14. Lee, B. H., W. S. Kim, W. W. Park, S. U. Jeong and H. J. Park, 1997, Study on the de­velopment of analytical method (multi-pesti­cide residue method) for the organophosphate pesticides, J. Korean Envi. Sci. Soc., 6(2), 183-187
  15. Lee, S. M., M. L. Papathakis, H. M. C. Feng, G. F. Hunter and J. E. Carr, 1991, Multipes­ticide residue method for fruits and vegetable : California Department of Food and Agricul­ture, J. Anal. Chem., 339, 376-383 https://doi.org/10.1007/BF00322352
  16. Torres, C. M., Y. Pico, M. J. Redondo and J. Manes, 1996, Matrix solid-phase dispersion extraction procedure for multiresidue pesti­cide analysis in orange, J. Chromat. A, 719, 9S-103
  17. Torres, C. M., Y. Pico and J. Manes, 1995, Analysis of pesticide residues in fruit and vegetables by matrix solid phase dispersion (MSPD) and different gas chromatography element-selective detectors, Chromatographia., 41, 685-692 https://doi.org/10.1007/BF02688108
  18. IUPAC Commission on Pesticide Chemistry, 1986, Pure & Appl. Chrm., 58, 1035 https://doi.org/10.1351/pac198658071035
  19. Kim, W. S., B. H. Lee and H. J. Park, 1996, Study on the development of simultaneous analytical method for the residual organo­chloride pesticide by gas chromatography, J. Korean Envi. Sci. Soc., 5, 561-567