DOI QR코드

DOI QR Code

Effects of Water Extract from Enzymic-Treated Hamcho (Salicornia herbacea) on Lipid Metabolism in Rats Fed High Cholesterol Diet

효소처리한 함초(Salicornia herbacea) 열수추출물이 고콜레스테롤 식이 흰쥐의 지질대사에 미치는 영향

  • Kim, Kyung-Ran (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Jang, Mi-Jin (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Choi, Sang-Won (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Woo, Mi-Hee (Dept. of Pharmacy, Catholic University of Daegu) ;
  • Choi, Jeong-Hwa (School of Food Science, Jinju International University)
  • 김경란 (대구가톨릭대학교 식품영양학과) ;
  • 장미진 (대구가톨릭대학교 식품영양학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과) ;
  • 우미희 (대구가톨릭대학교 약학과) ;
  • 최정화 (진주국제대학교 식품과학부)
  • Published : 2006.01.01

Abstract

This study was conducted to investigate the effects of water extract from enzymic-treated Salicornia herbacea (Hamcho) on lipid metabolism in rats fed high cholesterol diet. Experimental rats were divided into six groups which were composed of normal diet group, normal and $2\%$ Hamcho extract diet group, high cholesterol diet group, high cholesterol and $1\%,\;2\%\;and\;4\%$ Hamcho extracts diet groups. Body weight gain and food efficiency ratio (FER) of the high cholesterol diet group were significantly increased compared to the normal diet group, while those of the high cholesterol and Hamcho extracts diet groups were decreased compared to the high cholesterol diet group. Food intake was not significantly different among all experimental groups, The serum TG content of the high cholesterol diet group was significantly increased compared to the normal diet group, but that of the high cholesterol and extracts diet group was significantly decreased in dose-dependent concentration. The liver TG, total lipid and total cholesterol contents of the high cholesterol diet group were significantly increased compared to the normal diet group, while those of the high cholesterol and $2\%\;or\;4\%$ Hamcho extracts diet groups were significantly decreased compared to the high cholesterol diet group. The serum total cholesterol and LDL-cholesterol contents, and AI of the high cholesterol diet group were significantly increased compared to the normal diet group, while those of the high cholesterol and Hamcho extracts diet groups were decreased, and especially the high cholesterol and $4\%$ Hamcho extract diet group was significantly decreased compared to the high cholesterol diet group. Serum HDL-cholesterol contents of the high cholesterol diet group were significantly increased compared to the normal diet group. These results suggest that supplementation of water extract from enzymic-treated Salicornia herbacea may have a pronounced impact on lipid metabolism of serum and liver in the rats fed high cholesterol diet.

본 연구에서는 고콜레스테롤 식이 흰쥐에서 효소 처리하여 제조된 함초 열수추출물의 공급이 혈청 및 간조직의 지질대사에 미치는 영향을 관찰하였다. 각 실험군의 100g당 간 조직의 무게를 비교한 결과 정상식이 대조군과 정상식이에 함초 추출물을 공급한 군은 유의적인 차이가 없었으며, 고콜레스테롤군들에서 정상식이 대조군에 비해 유의적으로 증가하였다. 간조직 중성지질 함량은 고콜레스테롤군들에서 정상식이 대조군에 비해 유의적으로 증가하였으며, 고콜레스테롤식이에 함초 추출물 $2\%$$4\%$를 공급한 군들에서 고콜레스테롤식이 대조군보다 유의적으로 감소되었으며, 간조직 총지질 함량 또한 중성지질과 같은 경향을 나타내었다. 혈청 총콜레스테롤 함량은 고콜레스테롤 식이대조군에서 정상식이 대조군에 비해 유의적으로 증가하였으나, 고콜레스테롤식이에 함초 추출물을 공급함으로써 총콜레스테롤 함량이 다소 감소하였다. 간조직 총콜레스테롤의 함량은 혈청 총 콜레스테롤 함량과 유사한 경향을 나타내었으며, 고콜레스테롤식이에 함초 추출물 $2\%$$4\%$를 공급한 군들에서 고콜레스테롤식이 대조군에 비해 콜레스테롤의 함량이 유의적으로 감소되었다. 혈청 HDL-콜레스테롤 함량은 고콜레스테롤군들에서 정상식이 대조군에 비해 유의적으로 감소하였으며, 고콜레스테롤식이에 함초 추출물을 공급한 군들에서 다소 증가하였다. 혈청 LDL-콜레스테롤 함량은 고콜레스테롤식이 대조군이 정상식이 대조군에 비해 유의적으로 증가하였으나, 고콜레스테롤식이에 함초 추출물 $4\%$를 공급한 군에서 고콜레스테롤식이 대조군에 비해 유의적으로 감소되었다. 따라서, 고콜레스테롤 식이 흰쥐에서 효소 처리한 함초 열수추출물의 공급은 혈청 및 간조직의 총지질, 중성지질, 총콜레스테롤, LDL-콜레스테롤 및 HDL-콜레스테롤 등의 지질함량을 조절함으로써 지질대사 개선 효과가 있음을 알 수 있었다.

Keywords

References

  1. Kim DS, Kim CH. 2001. Effect of sea tangle, Laminaria japonicus, extract on activities of glucokinase and hexo-kinase in alloxan-induced diabetic mellitus mice. Korean J Life Sci 11: 467-482
  2. Han SK. 2004. Antioxidative effect of fermented Salicornia Herbacea L. liquid with EM (effective microorganism) on pork. Korean J Food Sci Ani Resour 24: 298-302
  3. Cho KJ, Lee YS, Ryu BH. 1990. Antitumor effect and im-munology activity seaweeds toward sarcoma-180. J Kor Fish Soc 23: 345-352
  4. Lee HS, Choi MS, Lee YK, Park SH, Kim YJ. 1996. A study on the development of high-fiber supplements for the diabetic patients (2)-Effect of seaweed supplementation on the lipid and glucose metabolism in streptozotocin-induced diabetic rats. Korean J Nutr 29: 296-306
  5. Lee EJ, Sung MK. 2001. Effect of fiber-rich sea mustard feeding on AOM-induced colon aberrant crypt formation and colonic cell proliferation in Sprague-Dawley rats. J Korean Soc Food Sci Nutr 30: 535-539
  6. Lee KS, Seo JS, Choi YS. 1998. Effect of sea tangle and hypoglycemic agent on lipid metabolism in diabetic rats. J Korean Soc Food Sci Nutr 27: 960-967
  7. Lee HJ, Kim YA, Ahn JW, Lee BJ, Moon SK, Seo YW. 2004. Screening of peroxynitrite and DPPH radical scav-en-ging activities from salt marsh plants. Korean J Biotechnol Bioeng 19: 57-61
  8. Adeghate E, Parvez SH. 2000. Nitric oxide and neuronal and pancreatic beta cell death. Toxicology 153: 143-156 https://doi.org/10.1016/0300-483X(75)90081-5
  9. Reddi AS. 1986. Riboflavin nutritional status and flavo-protein enzymes in streptozotocin-diabetic rats. Biochem Biophys Acta 3: 71-76
  10. Ihm BS, Lee JS. 1986. The strategies of Salicornia herbacea and Suaeda japonica for coping with environ-mental fluctu-ation of salt marsh. Korean J Environ Biol 4: 15-25
  11. Cha JY, Netty E, Kim SG, Lee JJ, Lim CO, Chung WS, Lee KH, Son DY. 2003. Molecular cloning and charac-terization of salt-inducible aldolase from Salicornia her-bacea. Korean J Plant Biotechnol 30: 323-328 https://doi.org/10.5010/JPB.2003.30.4.323
  12. Han SK, Kim SM. 2003. Antioxidative effect of Salicornia herbacea L. grown in closed sea beach. J Korean Soc Food Sci Nutr 32: 207-210 https://doi.org/10.3746/jkfn.2003.32.2.207
  13. Han SK, Kim SM, Pyo BS. 2003. Antioxidative effect of glasswort (Salicornia herbacea L.) on the lipid oxidation of pork. Korean J Food Sci Ani Resour 23: 46-49
  14. Netty E, Cha JY, Yingshi L, Jung MH, Shin DG, Lee BH, Lee KH, Son DY. 2004. Molecular cloning and char-acterization of outer envelope membrane protein from Salicornia herbacea. Korean J Plant Biotechnol 31: 273- 278 https://doi.org/10.5010/JPB.2004.31.4.273
  15. Jeong CY, Ryu JS, Choi CK, Jeon BS, Park JW, Kim BK, Shin GG, Bae DW, Cha JY. 2004. Supplemented effect of Salicornia herbacea extracted powder on preparation and quality characteristics of fermented milk product. J Life Sci 14: 788-793 https://doi.org/10.5352/JLS.2004.14.5.788
  16. Park SH, Kim KS. 2004. Isolation and identification of antioxidant flavonoids from Salicornia herbacea L. J Korean Soc Appl Biol Chem 47: 120-123
  17. Do JR, Kim EM, Koo JG, Jo KS. 1997. Dietary fiber contents of marine algae and extraction condition of the fiber. J Korean Fish Soc 30: 291-296
  18. Cho SY, Kang HJ, Joo DS, Lee JS, Kim SM. 1999. A comparative study on physical properties and gel formation abilities of hot-water extractable material, water-soluble alginate and alkali-soluble alginate extracted form Laminaria japonica in east sea, Korea. J Korean Fish Soc 32: 774-778
  19. Lee JT, An BJ. 2002. Detection of physical activity of Salicornia herbacea. Kor J Herbology 17: 61-69
  20. Min JG, Lee DS, Kim TJ, Park JH, Cho TY, Park DI. 2002. Chemical composition of Salicornia herbacea L. J Food Sci Nutr 7: 105-107 https://doi.org/10.3746/jfn.2002.7.1.105
  21. Cha JY, Jeon BS, Park JW, Kim BK, Jeong CY, Ryu JS, Choi CK, Cho YS. 2004. Hypocholesterolemic effect of yogurt supplemented Salicornia herbacea extrect in cho-lesterol-fed rats. J Life Sci 14: 747-751 https://doi.org/10.5352/JLS.2004.14.5.747
  22. Sale FD, Marchesini S, Fishman PH, Berra B. 1984. A sen-sitive enzymatic assay for determination of cholesterol in lipid extracts. Academic Press Inc, New York. p 347-350
  23. Folch JM, Lees M, Stanley GHS. 1957. A simple method for the isolation and purification of total lipld from animal tissue. J Biol Chem 26: 497-509
  24. Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502
  25. Fiordaliso M, Kok N, Desager KP, Goethals F, Deboyser D, Roberfoid M, Delzenne N. 1995. Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats. Lipids 30: 163- 167 https://doi.org/10.1007/BF02538270
  26. Sreel RGD, Torrie JH. 1990. Principles and procedures of satistics. McGrow Hill, New York, USA
  27. Rhee SJ, Park HK. 1984. Changes of lipid content and histochemical observation in liver of rats fed high fat diet. Korean J Nutr 17: 113-125
  28. Rhee IJ, Kim EJ, Jeong SW, Yang JH, Lee IS. 2003. Effects of Liriopis tuber extracts on lipid metabolism in rats fed high cholesterol diet. Korean J Pharmacogn 34: 65-69
  29. Jo YC, Ahn JH, Chon SM, Lee KS, Bae TJ, Kang DS. 2002. Studies on pharmacological effects of glasswort (Salicornia herbacea L.). J Medicinal Crop Sci 10: 93-99
  30. Kim YY, Lee KW, Kim GB, Cho YJ. 2000. Studies on physicochemical and biological properties of depolymerized alginate from sea tangle Laminaria japonicus by heating hydrolysis. 3. Excretion effects of cholesterol, glucose and cadmium (Cd) in rats. J Korean Fish Soc 33: 393-398
  31. Bang MA, Kim HA, Cho YJ. 2002. Hypoglycemic and antioxidant effect of dietary Hamcho powder in strepto-zotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 31: 840-846 https://doi.org/10.3746/jkfn.2002.31.5.840

Cited by

  1. Physiochemical Characteristics and Antioxidant Activity of Soybean Curd Added with Saltwort (Salicornia herbacea L.) vol.29, pp.2, 2013, https://doi.org/10.9724/kfcs.2013.29.2.123
  2. Hypolipidemic effect of Salicornia herbacea in animal model of type 2 diabetes mellitus vol.1, pp.4, 2007, https://doi.org/10.4162/nrp.2007.1.4.371
  3. Anti-obesity effect of Korean Hamcho (Salicornia herbaceaL.) powder on high-fat diet-induced obese rats vol.48, pp.2, 2015, https://doi.org/10.4163/jnh.2015.48.2.123
  4. Quality Characteristics of 4 Year-old Ginseng by Enzymatic Hydrolysis Conditions vol.40, pp.2, 2011, https://doi.org/10.3746/jkfn.2011.40.2.229
  5. Production of novel vinegar having antioxidant and anti-fatigue activities fromSalicornia herbaceaL. vol.96, pp.4, 2016, https://doi.org/10.1002/jsfa.7180
  6. Antioxidant and Anti-inflammatory Activities of Extracts from Korean Traditional Medicinal Prescriptions vol.43, pp.5, 2011, https://doi.org/10.9721/KJFST.2011.43.5.624
  7. Effects of Enzymatic Hydrolysates from Hamcho (Salicornia herbacea L.) on Blood Glucose and Serum Lipid Composition in Streptozotocin-Induced Diabetic Rats vol.37, pp.2, 2008, https://doi.org/10.3746/jkfn.2008.37.2.170
  8. Effects of Salicornia herbacea on Growth Performance, Meat Quality, Excreta Microbial Populations, and Noxious Gas Emissions in Broiler Chicks vol.56, pp.1, 2019, https://doi.org/10.2141/jpsa.0170210