Enhanced Heterologous Expression of Aspergillus niger Epoxide Hydrolase and Its Application to Enantioselective Hydrolysis of Racemic Epoxides

Aspergillus niger의 Epoxide Hydrolase 고효율 발현 및 라세믹 에폭사이드의 입체선택적 가수분해

  • Lee, Soo Jung (Department of Food Science and Technology, College of Engineering, Kyungsung University) ;
  • Kim, Hee Sook (Department of Food Science and Technology, College of Engineering, Kyungsung University) ;
  • Lee, Eun Yeol (Department of Food Science and Technology, College of Engineering, Kyungsung University)
  • 이수정 (경성대학교 공과대학 식품공학과) ;
  • 김희숙 (경성대학교 공과대학 식품공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과)
  • Received : 2005.08.25
  • Accepted : 2006.06.12
  • Published : 2006.10.10

Abstract

The epoxide hydrolase (EH) of Aspergillus niger LK was expressed to high levels in Escherichia coli based on codon usage. E. coli, Rosetta (DE3)PLysS, containing a large number of tRNAs for rare-codons was employed as a host strain. The recombinant E. coli expressing A. niger EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee was obtained from racemic substrates.

Keywords

epoxide hydrolase;Aspergillus niger;enantioselective hydrolysis;Escherichia coli Rosetta;codon usage

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