Optimum Condition of Mobile Phase Composition for Purine Compounds by HCI Program

HCI프로그램을 이용한 퓨린 유도체의 이동상 조성의 최적화 조건

  • Jin, Chun Hua (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Lee, Ju Weon (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
  • 김춘화 (인하대학교 화학공학과 인하대학교 초정밀생물분리기술연구센터) ;
  • 이주원 (인하대학교 화학공학과 인하대학교 초정밀생물분리기술연구센터) ;
  • 노경호 (인하대학교 화학공학과 인하대학교 초정밀생물분리기술연구센터)
  • Received : 2006.04.13
  • Accepted : 2006.05.17
  • Published : 2006.06.10

Abstract

The optimum mobile phase condition for analysis of the six purine derivatives (caffeine, guanine, hypoxanthine, purine, theobromine, and theophylline) were determined by a HCI program. Reversed-phase HPLC system was used with the binary mobile phase, water and methanol. Three retention models (Snyder, Langmuir, and Binary polynomial) were considered to predict the retention factors. The elution profiles were calculated by the plate theory based on the binary polynomial retention model. From the final calculated results, the binary polynomial retention model showed the best agreements between the calculated and experimental data. In the isocratic mode, the optimum mobile phase composition of water/methanol is 93/7(v/v). However, we used step-gradient mode to decrease the run-time ($1^{st}$ mobile phase : water/methanol = 93/7 (v/v), gradient time : 5 min, $2^{nd}$ mobile phase : water/methanol = 75/25 (v/v)). The experimental and simulated profiles of above the two conditions show a good agreement.

Acknowledgement

Supported by : 인하대학교

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