A Study on the Factors Affecting Entrapment Efficiency and Particle Size of Ethosomes

Ethosomes의 포집효율과 입자크기에 영향을 주는 인자에 관한 연구

  • Jin, Byung Suk (Department of Applied Chemistry, Dongduk Women's University) ;
  • Lee, Sang Mook (Department of Applied Chemistry, Dongduk Women's University) ;
  • Lee, Kwang Hee (Nano System Engineering, Inha University)
  • 진병석 (동덕여자대학교 응용화학과) ;
  • 이상묵 (동덕여자대학교 응용화학과) ;
  • 이광희 (인하대학교 나노시스템 공학부)
  • Received : 2005.10.10
  • Accepted : 2006.03.22
  • Published : 2006.04.10


Ethosome is a liquid crystalline vesicle prepared by hydration of ethanol-dissolved lecithin with a solution containing hydrophilic components. Investigation of factors affecting the entrapment efficiency and particle size of ethosomes was carried out, because the high entrapment efficiency and small particle size are prerequisite in developing ethosomes as a drug delivery system. The variations of properties of ethosomes with constituent composition and preparation method were examined using a calcein as a hydrophilic marker. It was observed that the amount of ethanol and calcein solution, phosphatidyl choline content in lecithin, preparation temperature, stirring rate, and PBS addition method had a considerable effect on the properties of ethosome. Sonication treatment resulted in the reduction of entrapment efficiency of ethosome, which was due to the release of entrapped components in the vesicles by strong sonication vibration.


Supported by : 동덕여자 대학교


  1. K. Egbaria and N. Weiner, Adv. Drug. Rev., 5, 287 (1990) https://doi.org/10.1016/0169-409X(90)90021-J
  2. V. M. Knepp, R. S. Hinz, F. C. Szoka, and R. H. Guy, J. Control. Rel., 5, 211 (1988) https://doi.org/10.1016/0168-3659(88)90020-X
  3. V. M. Knepp, F. C. Szoka, and R. H. Guy, J. Control. Rel., 12, 25 (1990) https://doi.org/10.1016/0168-3659(90)90179-W
  4. M. E. Planas, P. Gonzalez, L. Rodriquez, S. Sanchez, and G. Cevc, Anesth. Analg., 75, 615 (1992)
  5. G. Cevc and G. Blume, Biochim. Biophys. Acta, 1104, 226 (1992) https://doi.org/10.1016/0005-2736(92)90154-E
  6. G. Cevc, A. Schatzlein, and G. Blume, J. Control. Rel., 36, 3 (1995) https://doi.org/10.1016/0168-3659(95)00056-E
  7. G. Cevc, G. Blume, A. Schatzlein, D. Gebauer, and A. Paul, Adv. Drug Del. Rev., 18, 349 (1996) https://doi.org/10.1016/0169-409X(95)00091-K
  8. G. Cevc, G. Blume, and A. Schatzlein, J. Control. Rel., 45, 211 (1997) https://doi.org/10.1016/S0168-3659(96)01566-0
  9. A. Paul, G. Cevc, and B. K. Bachhawat, Eur. J. Immunol., 25, 3521 (1995) https://doi.org/10.1002/eji.1830251248
  10. M. Trotta, E. Peira, F. Debernardi, and M. Gallarate, Int. J. Pharm., 241, 319 (2002) https://doi.org/10.1016/S0378-5173(02)00266-1
  11. M. Trotta, E. Peira, M. E. Carlotti, and M. Gallarate, Int. J. Pharm., 270, 119 (2004) https://doi.org/10.1016/j.ijpharm.2003.10.006
  12. E. Touitou, N. Dayan, L. Bergelson, B. Godin, and M. Eliaz, J. Control. Rel., 65, 403 (2000) https://doi.org/10.1016/S0168-3659(99)00222-9
  13. E. Touitou, B. Godin, and C. Weiss, Drug Dev. Res., 50, 406 (2000) https://doi.org/10.1002/1098-2299(200007/08)50:3/4<406::AID-DDR23>3.0.CO;2-M
  14. E. Touitou, V. M. Meidan, and E. Horwitz, J. Control. Rel., 56, 7 (1998) https://doi.org/10.1016/S0168-3659(98)00060-1
  15. S. Perrett, M. Golding, and W. P. Williams, J. Pharm. Pharmacol., 43, 154 (1991) https://doi.org/10.1111/j.2042-7158.1991.tb06657.x
  16. M. Kirjavainen, A. Urtti, R. Valjakka-Koskela, J. Kiesvaara, and J. Monkkonen, Eur. J. Pharm. Sci., 7, 279 (1999) https://doi.org/10.1016/S0928-0987(98)00037-2
  17. B. S. Jin, Y. M. Han, K. H. Lee, and M. J. Choi, J. Korean Ind. Eng. Chem., 15, 205 (2004)