Analysis of Intragenic Ds Transpositions and Excision Events Generating Novel Allelic Variation in Rice

  • Park, Soon Ju (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Piao, Hai Long (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Xuan, Yuan-hu (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Park, Sung Han (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Je, Byoung Il (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Kim, Chul Min (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Lee, Eun Jin (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Park, Soo Hyun (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Ryu, ByeongChan (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Lee, Kon Ho (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University) ;
  • Lee, Gi Hwan (Division of Rice Breeding and Cultivation, Yeongnam National Agricultural Experiment Station) ;
  • Nam, Min Hee (Division of Rice Breeding and Cultivation, Yeongnam National Agricultural Experiment Station) ;
  • Yeo, Un Sang (Division of Rice Breeding and Cultivation, Yeongnam National Agricultural Experiment Station) ;
  • Lee, Myung Chul (Rice Functional Genomics Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Yun, Doh Won (Rice Functional Genomics Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Eun, Moo Young (Rice Functional Genomics Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Han, Chang-deok (Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University)
  • Received : 2006.01.11
  • Accepted : 2006.02.08
  • Published : 2006.04.30

Abstract

Even though Ac/Ds gene-tagging systems have been established in many higher plants, maize is the only major plant in which short-distance transposition of Ac/Ds has been utilized to probe gene function. This study was performed to evaluate the efficiency of obtaining new alleles and functional revertants from Ds insertion loci in rice. By analyzing 1,580 plants and the progeny of selected lines, the insertion sites and orientations of Ds elements within 16 new heritable alleles of three rice loci were identified and characterized. Intragenic transposition was detected in both directions from the original insertion sites. The closest interval was 35 bp. Three of the alleles had two Ds elements in cis configuration in the same transcription units. We also analyzed the excision footprints of intragenic and extragenic transpositions in Ds-inserted alleles at 5 loci. The 134 footprints obtained from different plants revealed predominant patterns. Ds excision at each locus left a predominant footprint at frequencies of 30-75%. Overall, 66% of the footprints were 7-bp additions. In addition, 16% of the excisions left 0-, 3-, 6-, and 9-bp additions with the potential of conserving reading frame.

Keywords

Ac;Ds;Footprints;Intragenic Transposition;Rice

Acknowledgement

Supported by : Crop Functional Genomics Center, Korea Research Foundation, KOSEF

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