Cellular Changes Resulting from Forced Expression of Glypican-3 in Hepatocellular Carcinoma Cells

  • Kwack, Mi Hee (Department of Immunology, School of Medicine, Kyungpook National University) ;
  • Choi, Byung Yeol (Department of Immunology, School of Medicine, Kyungpook National University) ;
  • Sung, Young Kwan (Department of Immunology, School of Medicine, Kyungpook National University)
  • Received : 2005.11.01
  • Accepted : 2006.02.27
  • Published : 2006.04.30


Glypican-3 (GPC3) is a member of the glypican family, which encodes cell-surface heparan-sulfate proteoglycans, and is frequently upregulated in hepatocellular carcinoma (HCC). We have recently reported that blocking endogenous GPC3 expression promotes the growth of HCC cell lines, suggesting that GPC3 plays a negative role in HCC cell proliferation. Here, we report that forced expression of GPC3 reduced the growth of HCC cells. We also found that FGF2-mediated cell proliferation was inhibited by GPC3. In addition, we observed that the adhesion of HCC cells to collagen type I and fibronectin was decreased by GPC3, whereas cellular migration and invasiveness were stimulated. Collectively, these results suggest that progression of hepatocellular carcinoma is associated with upregulation of GPC3.


Adhesion;Glypican-3;Growth Suppression;Hepatocellular Carcinoma;Invasion;Migration


Supported by : Kyungpook National University Hospital


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