Plastid Transformation in the Monocotyledonous Cereal Crop, Rice (Oryza sativa) and Transmission of Transgenes to Their Progeny

  • Lee, Sa Mi (School of Agricultural Biotechnology, Seoul National University) ;
  • Kang, Kyungsu (School of Agricultural Biotechnology, Seoul National University) ;
  • Chung, Hyunsup (School of Agricultural Biotechnology, Seoul National University) ;
  • Yoo, Soon Hee (School of Agricultural Biotechnology, Seoul National University) ;
  • Ming Xu, Xiang (School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Seung-Bum (Department of Molecular Biology and Microbiology, University of Central Florida) ;
  • Cheong, Jong-Joo (School of Agricultural Biotechnology, Seoul National University) ;
  • Daniell, Henry (Department of Molecular Biology and Microbiology, University of Central Florida) ;
  • Kim, Minkyun (School of Agricultural Biotechnology, Seoul National University)
  • Received : 2006.02.22
  • Accepted : 2006.03.28
  • Published : 2006.06.30

Abstract

The plastid transformation approach offers a number of unique advantages, including high-level transgene expression, multi-gene engineering, transgene containment, and a lack of gene silencing and position effects. The extension of plastid transformation technology to monocotyledonous cereal crops, including rice, bears great promise for the improvement of agronomic traits, and the efficient production of pharmaceutical or nutritional enhancement. Here, we report a promising step towards stable plastid transformation in rice. We produced fertile transplastomic rice plants and demonstrated transmission of the plastidexpressed green fluorescent protein (GFP) and aminoglycoside 3′-adenylyltransferase genes to the progeny of these plants. Transgenic chloroplasts were determined to have stably expressed the GFP, which was confirmed by both confocal microscopy and Western blot analyses. Although the produced rice plastid transformants were found to be heteroplastomic, and the transformation efficiency requires further improvement, this study has established a variety of parameters for the use of plastid transformation technology in cereal crops.

Keywords

Cereal Crop;Chloroplast Genome;Monocotyledonous Plant pecies;Plastid Transformation;Rice

Acknowledgement

Supported by : USDA

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