Identification of Two Types of Naturally-occurring Intertypic Recombinants of Epstein-Barr Virus

  • Kim, Sung-Min (Division of Bioscience and Bioinformatics and Institute of Bioscience and Biotechnology, Myongji University) ;
  • Kang, So-Hee (Division of Bioscience and Bioinformatics and Institute of Bioscience and Biotechnology, Myongji University) ;
  • Lee, Won-Keun (Division of Bioscience and Bioinformatics and Institute of Bioscience and Biotechnology, Myongji University)
  • Received : 2005.10.14
  • Accepted : 2006.01.25
  • Published : 2006.04.30

Abstract

Two Epstein-Barr virus (EBV) types, type 1 and type 2, maintain the same allelic specificity at four genomic loci encoding the EBNA2, -3A, -3B, and -3C proteins. We have previously described 16 EBV-transformed B-lymphoblastoid cell lines derived from Korean cancer patients, and the EBNA2 types of the EBV isolates therein. In this study, the allelic types of the EBNA2, -3A, -3B, and -3C genes of these EBV isolates were determined. We report the identification of two distinct types of naturally occurring intertypic recombinants, one with genotype EBNA2 type1/EBN3A, -3B, -3C type 2 and the other with genotype EBNA2, -3A type 1/EBNA3B, -3C type 2. The existence of these intertypic recombinants indicates that various intertypic EBV strains may be circulating in the human population, in addition to typical EBV-1 and EBV-2 strains.

Keywords

B-Lymphoblastoid Cell Line;Epstein-Barr Virus;Intertypic Recombinant;Type Determination

Acknowledgement

Supported by : Ministry of Education of Korea

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