Identification of Two Types of Naturally-occurring Intertypic Recombinants of Epstein-Barr Virus

  • Kim, Sung-Min (Division of Bioscience and Bioinformatics and Institute of Bioscience and Biotechnology, Myongji University) ;
  • Kang, So-Hee (Division of Bioscience and Bioinformatics and Institute of Bioscience and Biotechnology, Myongji University) ;
  • Lee, Won-Keun (Division of Bioscience and Bioinformatics and Institute of Bioscience and Biotechnology, Myongji University)
  • Received : 2005.10.14
  • Accepted : 2006.01.25
  • Published : 2006.04.30


Two Epstein-Barr virus (EBV) types, type 1 and type 2, maintain the same allelic specificity at four genomic loci encoding the EBNA2, -3A, -3B, and -3C proteins. We have previously described 16 EBV-transformed B-lymphoblastoid cell lines derived from Korean cancer patients, and the EBNA2 types of the EBV isolates therein. In this study, the allelic types of the EBNA2, -3A, -3B, and -3C genes of these EBV isolates were determined. We report the identification of two distinct types of naturally occurring intertypic recombinants, one with genotype EBNA2 type1/EBN3A, -3B, -3C type 2 and the other with genotype EBNA2, -3A type 1/EBNA3B, -3C type 2. The existence of these intertypic recombinants indicates that various intertypic EBV strains may be circulating in the human population, in addition to typical EBV-1 and EBV-2 strains.


B-Lymphoblastoid Cell Line;Epstein-Barr Virus;Intertypic Recombinant;Type Determination


Supported by : Ministry of Education of Korea


  1. Burrows, J. M., Khanna, R., Sculley, T. B., Alpers, M. P., Moss, D. J., et al. (1996) Identification of a naturally occurring recombinant Epstein-Barr virus isolate from New Guinea that encodes both type 1 and type 2 nuclear antigen sequences. J. Virol. 70, 4829-4833
  2. Lee, W.-K., Kim, S. M., Shim, Y. S., Cho, S.-G., Park, S. H., et al. (1998) B-lymphoblastoid cell lines from cancer patients. In Vitro Cell. Dev. Biol. Anim. 34, 97-100
  3. Lee, W., Hwang, Y. H., Lee, S. K., Subramanian, C., and Robertson, E. S. (2001) An Epstein-Barr virus isolated from a lymphoblastoid cell line has a 16-kilobase-pair deletion which includes gp350 and the Epstein-Barr virus nuclear antigen 3A. J. Virol. 75, 8556-8568
  4. Young, L. S., Yao, Q. Y., Rooney, C. M., Sculley, T. B., Moss, D. J., et al. (1987) New type B isolates of Epstein-Barr virus from Burkitt's lymphoma and from normal individuals in endemic areas. J. Gen. Virol. 68, 2853-2862
  5. Shim, Y. S., Kim, C. W., and Lee, W.-K. (1998) Sequence variation of EBNA2 of Epstein-Barr virus isolates from Korea. Mol. Cells 8, 226-232
  6. Cho, S.-G. and Lee, W.-K. (2000) Analysis of genetic polymorphisms of Epstein-Barr virus isolates from cancer patients and healthy carriers. J. Microbiol. Biotechnol. 10, 620-627
  7. Cohen, J. I., Wang, F., Mannick, J., and Kieff, E. (1989) Epstein- Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation. Proc. Natl. Acad. Sci. USA 86, 9558-9562
  8. Midgley, R. S., Blake, N. W., Yao, Q. Y., Croom-Carter, D., Cheung, S. T., et al. (2000) Novel intertypic recombinants of Epstein-Barr virus in the Chinese population. J. Virol. 74, 1544-1548
  9. Baer, R., Bankier, A. T., Biggin, M. D., Deininger, P. L., Farrell, P. J., et al. (1984) DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310, 207-211
  10. Sidagis, J., Ueno, K., Tokunaga, M., Ohyama, M., and Eizuru, Y. (1997) Molecular epidemiology of Epstein-Barr virus (EBV) in EBV-related malignancies. Int. J. Cancer 72, 72-76<72::AID-IJC11>3.0.CO;2-C
  11. Yao, Q. Y., Tierney, R. J., Croom-Carter, D., Cooper, G. M., Ellis, C. J., et al. (1996) Isolation of intertypic recombinants of Epstein-Barr virus from T-cell-immunocompromised individuals. J. Virol. 70, 4895-4903
  12. Arrand, J. R., Young, L. S., and Tugwood, J. D. (1989) Two families of sequences in the small RNA-encoding region of Epstein-Barr virus (EBV) correlate with EBV types A and B. J. Virol. 63, 983-986
  13. Rabson, M., Gradoville, L., Heston, L., and Miller, G. (1982) Non-immortalizing P3J-HR-1 Epstein-Barr virus: a deletion mutant of its transforming parent, Jijoye. J. Virol. 44, 834-844
  14. Rowe, M., Young, L. S., Cadwallader, K., Petti, L., Kieff, E., et al. (1989) Distinction between Epstein-Barr virus type A (EBNA 2A) and type B (EBNA 2B) isolates extends to the EBNA 3 family of nuclear proteins. J. Virol. 63, 1031-1039
  15. Rickinson, A. B. and Kieff, E. (2001) Epstein-Barr virus; in Fields Virology, Knipe, D. M., Howley, P. M., Griffin, D. E., Lamb, R. A., Martin, M., Roizman, A. B., and Straus, S. E. (eds.), 4th ed., Vol. 2, pp. 2575-2628, Lippincott Williams & Wilkins, Philadelphia, Pa
  16. Kunimoto, M., Tamura, S., Tabata, T., and Yoshie, O. (1992) One-step typing of Epstein-Barr virus by polymerase chain reaction: predominance of type 1 virus in Japan. J. Gen. Virol. 73, 455-461
  17. Rickinson, A. B., Young, L. S., and Rowe, M. (1987) Influence of the Epstein-Barr virus nuclear antigens EBNA-2 on the growth phenotype of virus-transformed B cells. J. Virol. 61, 1310-1317
  18. Dambaugh, T., Hennessy, K., Chamnankit, L., and Kieff, E. (1984) U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2. Proc. Natl. Acad. Sci. USA 81, 7632-7636
  19. Abdel-Hamid, M., Chen, J.-J., Constantine, N., Massoud, M., and Raab-Traub, N. (1992) EBV strain variation: geographical distribution and relation to disease state. Virology 190, 168-175
  20. Apolloni, A. and Sculley, T. B. (1994) Detection of A-type and B-type Epstein-Barr virus in throat washings and lymphocytes. Virology 202, 978-981
  21. Sample, J., Young, L., Martin, B., Chatman, T., Kieff, E., et al. (1990) Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes. J. Virol. 64, 4084-4092