Analysis on Association of a SNP in the Chicken OBR Gene with Growth and Body Composition Traits

  • Wang, Ying (College of Animal Science, Northeast Agricultural University) ;
  • Li, Hui (College of Animal Science, Northeast Agricultural University) ;
  • Zhang, YuanDan (Animal Genetics and Breeding Unit, the University of NewEngland, Armidale) ;
  • Gu, ZhiLiang (College of Animal Science, Northeast Agricultural University) ;
  • Li, ZhiHui (College of Animal Science, Northeast Agricultural University) ;
  • Wang, QiGui (College of Animal Science, Northeast Agricultural University)
  • Received : 2006.02.25
  • Accepted : 2006.08.19
  • Published : 2005.12.01


Leptin receptor (OBR) is a member of the class I cytokine receptor family. It signals mainly via the JAK/STAT pathway and plays an important role in regulating body energy storage and metabolism. This study was designed to investigate the effects of the OBR gene on chicken growth and body composition. Broiler lines selected divergently for or against abdominal fat were used. Primers for the exon9-region in the OBR gene were designed using chicken genomic sequences from the public genome domain. A C/A single nucleotide polymorphism (SNP) was found and its three genotypes (AA, AB and BB) were identified in this population. The results showed that the OBR polymorphism was associated with fatness traits, such as abdominal fat weight and abdominal fat percentage. This research suggests that OBR or a linked gene has effect on fat deposition in the chicken.


  1. Boldman, K. and D. V. Vleck. 2002. MTDFREML manual, United States Department of Agriculture USA
  2. Chen, C. C., T. Chang and H. Y. Su. 2004. Characterization of porcine leptin receptor polymorphisms and their association with reproduction and production traits. Anim. Biotechnol. 15 (1): 89-102
  3. Dunn, I. C., T. Boswell, M. Friedman-Einat, Y. Eshdat, D. W. Burt and I. R. Paton. 2000. Mapping of the leptin receptor gene (lepr) to chicken chromosome 8. Anim. Genet. 31(4): 290-295
  4. Martin, A., E. A. Dunnington, W. B. Gross, W. E. Briles, R. W. Briles and P. B. Siegel. 1990. Production traits and alloantigen systems in lines of chickens selected for high or low antibody responses to sheep erythrocytes. Poult. Sci. 69(6): 871-878
  5. Ohkubo, T., M. Tanaka and K. Nakashima. 2000. Structure and tissue distribution of chicken leptin receptor (cOB-R) mRNA. Biochim. Biophys. Acta. 1491(1-3):303-308
  6. Pinard-van, D. L. H. M., P. B. Siegel and S. J. Lamont. 1998. Lessons from selection experiments on immune response in the chicken. Poult. Avi. Bio. Rev. 9: 125-141
  7. Gu, Z. L., J. G. Zhao, H. Li, H. Meng, Q. Q. Wang, Q. H. Wang, and D. H. Zhu. 2002 Single Nucleotide Polymorphism Analysis in Chicken Leptin Receptor Exon9. Hereditas (Beijing) 24 (3): 259-262
  8. Meng, H., J. G. Zhao, Z. H. Li and H. Li. 2005. Single Nucleotide Polymorphisms on Peroxisome Proliferator-activated Receptor Genes Associated with Fatness Traits in Chicken. Asian-Aust. J. Anim. Sci. 18(9):1221-1225
  9. Horev, G., P. Einat, T. Aharoni, Y. Eshdat and M. Friedman-Einat. 2000. Molecular cloning and properties of the chicken leptinreceptor (CLEPR) gene. Mol. Cell Endocrnol. 162(1-2):95-106
  10. Li, H., N. Deeb, H. Zhou, A. D. Mitchell, C. M. Ashwell and S. J. Lamont. 2003. Chicken quantitative trait loci for growth and body composition associated with transforming growth factor- $\beta$ genes. Poult. Sci. 82(3): 347-356
  11. Zhang, Y., R. Proenca, M. Maffel, M. Barone, L. Leopold and J. M. Frideman. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 374(6251):425-431
  12. Li, C. Y. and H. Li. 2006. Association of MC4R Gene Polymorphisms with Growth and Body Composition Traits in Chicken. Asian-Aust. J. Anim. Sci. 19(6):763-768
  13. Wang, W. J., K. Ouyang, J. Ouyang, H. Li, S. Lin and H. Sun. 2004. Polymorphism of Insulin-like Growth Factor I Gene in Six Chicken Breeds and Its Relationship with Growth Traits. Asian-Aust. J. Anim. Sci. 17(3):301-305
  14. Tartaglia, L. A., M. Dembski, X. Weng, N. Deng, J. Culpepper, R. Devos, G. J. Richards, L. A. Campfield, F. T. Clark and J. Deeds. 1995. Identification and expression cloning of a leptin receptor, OB-R Cell 83(7):1263-1271
  15. Houseknecht, K. L. and C. P. Portacarrero. 1998. Leptin and its receptors: regulators of whole-body energy homeostasis. Domest. Anim. Endocrinol. 15(6): 457-475
  16. Quinton, N. D., A. J. Lee, R. J. Ross, R. Eastell and A. I. Blakemore. 2001. A single nucleotide polymorphism (SNP) in the leptin receptor is associated with BMI, fat mass and leptin levels in postmenopausal Caucasian women. Hum. Genet. 108 (3):233-236
  17. Rothschild, M. F. and M. Soller. 1997. Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe Newslett. Agric. Genom. 8:13-20
  18. Tartaglia, L. A. 1997. The leptin receptor. J. Biol. Chem. 272(10): 6093-6097
  19. Fei, H., H. J. Okano, C. Li, G. H. Lee, C. Zhao, R. Darnell and J. M. Friedman. 1997. Anatomic localization of alternatively spliced leptin receptors (Ob-R) in mouse brain and other tissues. Proc. Natl. Acad. Sci. 94(13): 7001-7005
  20. SAS Institute Inc. 2002. JMP User's Guide. Cary, NC: SAS Institute Inc

Cited by

  1. Novel SNPs of the Bovine LEPR Gene and Their Association with Growth Traits vol.46, pp.11-12, 2008,
  2. Recent Progress in Avian Leptin Research vol.51, pp.4, 2014,
  3. Association of leptin receptor gene polymorphisms with growth and feed efficiency in meat-type chickens vol.93, pp.8, 2014,
  4. genes in chicken preadipocytes vol.88, pp.3, 2016,