Influence of Temperature Shifts on Antibody Synthesis in the Oliver Flounder (Paralichthys olivaceus) Immunised with Formalin Killed Edwardsiella tarda Antigen

  • Published : 2006.12.01

Abstract

The effects of various temperature shifts on the kinetics of the humoral antibody response in oliver flounder, Paralichthys olivaceus, immunised with formalin-killed Edwardsiella tarda, were determined by measuring the antibody production in vivo and in vitro. When fish acclimated to a high temperature and immunised at that temperature were transferred to a lower temperature (22℃ to 12℃) at a various times after immunisation, the fish showed a weaker immune response than that achieved when the fish were kept at a high environmental temperature. However, in the converse experiment (12℃ to 22℃), the magnitude of the humoral immune response was recovered independent of the time of the transfer after immunisation at low temperature, even though the peak levels of each transferred group did not reach the level found in the positive control group that was maintained and immunised at a high environmental temperature. Hence, these studies provide some evidence that the potential for antibody production in B cells of oliver flounder immunized at high temperature is not impaired by subsequent exposure to low temperature.

References

  1. Alcorn, S. W., Murra, A. L. and Pascho, R. J.: Effects of rearing temperature on immune functions in sockeye salmon (Oncorhynchus nerka). Fish Shellfish Immunol., 12: 303-334,2002 https://doi.org/10.1006/fsim.2001.0373
  2. Attia-EI-Hili, H., EI-Gaaid, A., Troutaud, D. and Deschaux, P.: Study of reared sea bream antibody response to a sheep red blood cells injection. Ichtyophysiol. Acta, 15: 7-20, 1992
  3. Bly, J. E. and Clem, L. W: Temperature-mediated processes in teleost immunity : Hemeoviscous adaptation by channel catfish peripheral blood cells. Comp. Biochem. Physiol., 914: 481-485,1988
  4. Bly, J. E. and Clem, L. W: Temperature-mediated processes in teleost immunity: in vitro immunosuppression induced by in vivo low temperature in channel catfish. Vet.Immunol. Immunopathol., 28: 365-377, 1991 https://doi.org/10.1016/0165-2427(91)90127-X
  5. Bly, J. E. and Clem, L. W: Temperature and teleost immune functions. Fish Shellfish Immunol., 2: 159-171, 1992 https://doi.org/10.1016/S1050-4648(05)80056-7
  6. Engelsma,M. Y, Hougee, S., Nap, D., Hofenk, M., Rombout, J. H., van Muiswinkel, W. B. and Verburg-van Kemenade, B. M. L.: Multiple acute temperature stress affects leucocyte populations and antibody responses in common carp, Cyprinus carpio L. Fish Shellfish Irnmunol., 15: 397-410,2003 https://doi.org/10.1016/S1050-4648(03)00006-8
  7. Miller, N. W.and Clem, L. W.: Temperature-mediated processes in teleost immunity: differential effects of temperature on catfish in vitro antibody responses to TD and TI antigens. J.Immunol., 133:2356-2359, 1984
  8. Nath, S., Kales, S., Fujiki, K. and Dixon, B.: Major histocompatibility class Il genes in rainbow trout (Oncorhynchus mykiss) exhibittemperature dependent downregulation. Immunogenetics, 58: 443-453, 2006 https://doi.org/10.1007/s00251-006-0094-5
  9. Pylkko, P., Lyytikainen, T, Ritola, O., Pelkonen, S. and Valtonen, E. T: Temperature effect on the immune defense functions of Arctic charr Salvelinus alpinus. Dis. Aquat. Org., 52: 47-55, 2002 https://doi.org/10.3354/dao052047
  10. Rijkers, G. T, Frederix-Wolters, E. M. H. and Van Muinswinkel, W B.: The immune system of cyprinid fish. Kinetics and temperature dependence of antibody producing cells in carp (Cyprinus carpio). Immunol., 41: 9197,1980
  11. Secombes, C. J., White, A.,Fletcher, T. C. and Houlihan, D. F: The development of an ELISPOT assay to quantify total and specific antibody secreting cells in dab Limanda limanda (L). Fish Shellfish Immunol., 1: 87-89,1991 https://doi.org/10.1016/S1050-4648(06)80009-4
  12. Waterstrat, P. R., Ainsworth, A. J. and Capley, G.: In vitro responses of channel catfish (Ictalurus punctatusi neutrophils to Edwardsiella ictaluri. Dev. Comp. Immunol., 15: 53-63, 1991 https://doi.org/10.1016/0145-305X(91)90047-3
  13. Watts, M., Munday, B. L. and Burke C. M.: Immune responses of teleost fish. Aust. Vet.J., 79: 570-574,2001 https://doi.org/10.1111/j.1751-0813.2001.tb10753.x
  14. Yang, X. and Zuo, W.: Mechanism of water temperature-dependent immune response in grass carp (Ctenopharyngodon idella), J. Chin.Acad. Fish. Sci. Zhongguo Shuichan Kexue Yanjiuyuan Xuebao.,4: 27-33, 1991