Myogenic Differentiation of p53- and Rb-deficient Immortalized and Transformed Bovine Fibroblasts in Response to MyoD

  • Jin, Xun (Laboratory of Cell Growth and Function Regulation, Korea University) ;
  • Lee, Joong-Seub (Laboratory of Cell Growth and Function Regulation, Korea University) ;
  • Kwak, Sungwook (Laboratory of Cell Growth and Function Regulation, Korea University) ;
  • Jung, Ji-Eun (Laboratory of Cell Growth and Function Regulation, Korea University) ;
  • Kim, Tae-Kyung (Laboratory of Cell Growth and Function Regulation, Korea University) ;
  • Xuo, Chenxiong (Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Hong, Zhongshan (Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Li, Zhehu (Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Sun-Myoung (Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Whang, Kwang Youn (Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Hong, Ki-Chang (Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • You, Seungkwon (Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Choi, Yun-Jaie (Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Hyunggee (Laboratory of Cell Growth and Function Regulation, Korea University)
  • Received : 2005.10.12
  • Accepted : 2005.12.25
  • Published : 2006.04.30

Abstract

We have established in culture a spontaneously immortalized bovine embryonic fibroblast (BEF) cell line that has lost p53 and $p16^{INK4a}$ functions. MyoD is a muscle-specific regulator capable of inducing myogenesis in a number of cell types. When the BEF cells were transduced with MyoD they differentiated efficiently to desmin-positive myofibers in the presence of 2% horse serum and 1.7 nM insulin. The myogenic differentiation of this cell line was more rapid and obvious than that of C2C12 cells, as judged by morphological changes and expression of various muscle regulatory factors. To confirm that lack of the p53 and $p16^{INK4a}$ pathway does not prevent MyoD-mediated myogenesis, we established a cell line transformed with SV40LT (BEFV) and introduced MyoD into it. In the presence of 2% horse serum and 1.7 nM insulin, the MyoD-transduced BEFV cells differentiated like the MyoD-transduced BEFS cells, and displayed a similar pattern of expression of muscle regulatory proteins. Taken together, our results indicate that MyoD overexpression overcomes the defect in muscle differentiation associated with immortalization and cell transformation caused by the loss of p53 and Rb functions.

Keywords

BEF;Muscle Differentiation;MyoD p53;Rb;SV40LT

Acknowledgement

Supported by : ARPC, Ministry of Education and Human Resources Development in Korea

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