Short-term Hypothermic Preservation of CHO Cells Using Serum-Free Media

무혈청 배지를 이용한 CHO 세포의 단기 저온보존

  • Byoun, Soon-Hwi (Department of Microbial Engineering, Konkuk University) ;
  • Park, Hong-Woo (Department of Chemical Engineering, Hanyang University) ;
  • Choe, Tae-Boo (Department of Microbial Engineering, Konkuk University)
  • 변순휘 (건국대학교 미생물공학과) ;
  • 박홍우 (한양대학교 화학공학과) ;
  • 최태부 (건국대학교 미생물공학과)
  • Published : 2006.08.30

Abstract

Cell preservation is indispensable in animal cell culture process and should be established according to the cell characteristics. In this study, we experimented hypothermic preservation of CHO cells that is widely used in pharmaceutical industry to produce therapeutic proteins and established a stable method of preservation. The highest viability of CHO cells was obtained when the cells were preserved using rolling tube, which means the cells should be suspended to avoid the cell lumping during the preservation. Also, we obtained superior preservation result under the anaerobic condition. To evaluate the serum-free media as a preservation solution, we investigated cell growth after hypothermic preservation using serum-free media. High cell viability and normal cell growth was observed during 10 days using serum-free media. Moreover, we found that more effective preservation when ${\alpha}$-tocopherol and retinoic acid is added to media as an additive. In the case of 1 liter large scale hypothermic preservation using established protocol, cell viability and growth rate was obtained as good as small scale one. This study is considered to be helpful for hypothermic preservation of CHO cells and large scale hypothermic preservation may be available through the further studies.

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