Effects of Cheonggukjang on Immune Responses and Gastrointestinal Functions in Rats

  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Yang, Eun-In (Faculty of Biotechnology (Food Science & Technology Major), Chonbuk National University) ;
  • Song, Geun-Seoup (Department of Food Engineering, Iksan National College) ;
  • Chai, Ok-Hee (Department of Anatomy, Chonbuk National University Medical School) ;
  • Kim, Young-Soo (Faculty of Biotechnology (Food Science & Technology Major), Chonbuk National University)
  • Published : 2006.02.28


Effects of cheonggukjang on immunohistochemical reactions in gastrointestinal (GI) tract of rats were investigated. $CD4^+/CD8^+$ immunoreactive cells of cheonggukjang-fed diet groups were more strongly stained in lamina propria of mucosa and submucosa than those of basal diet group. Universal nitric oxide synthase immunoreactive density in colon was mildly stained in surface epithelium and mucous secretory gland, and strongly stained in submucosa and myenteric plexus in muscle layers of all cheonggukjang-fed diet groups. Protein kinase C-${\alpha}$ immunoreactive cells in colons of 15 and 25% cheonggukjang-fed diet groups were more strongly stained in mucosa, submucosa, and muscle layers than those of basal diet group. These results indicate mucosal immune activity, gastrointestinal motility, blood circulation, and physiological activities of enteroendocrine cells in GI tract could be increased with cheonggukjang intake.


  1. Katagiri F, Itoh H, Takeyama M. Effect of Sho-hange-ka-bukuryo-to on gastrointestinal peptide concentrations in the plasma of healthy human subjects. Biol. Pharm. Bull. 27: 1674-1678 (2004) https://doi.org/10.1248/bpb.27.1674
  2. Leeson TS, Leeson C, Paparo AA. Text/atlas of histology. W.B. Saunders Company, USA, p. 431 (1988)
  3. Klein JR, Kagnoff MF. Nonspecific recruitment of cytotoxic effector cells in the intestinal mucosa of antigen-primed mice. J. Exp. Med. 160: 1931-1936 (1984) https://doi.org/10.1084/jem.160.6.1931
  4. Ernst PB, Clark DA, Rosenthal KL, Befus AD, Bienenstock J. Detection and characterization of cytotoxic T lymphocyte precursors in the murine intestinal intraepithelial leukocyte population. J. Immunol. 136: 2121-2126 (1986)
  5. Taguchi T, McGhee JR, Coffinan RL, Beagly KW, Eldridge JH, Takatsu K, Kiyono H. Analysis Th1 and Th2 cells in murine gut associated tissue: frequencies of CD4+ and CD8+ T cells that secrete IFN-y and IL-5. J. Immunol. 145: 68-77 (1990)
  6. Ishizuka S, Tanaka S. Modulation of CD8+ intraepithelial lymphocyte distribution by dietary fiber in the rat large intestine. Exp. Biol. Med. 227: 1017-1021 (2002) https://doi.org/10.1177/153537020222701110
  7. Lee EJ, Kim JK. Characteristics of taste components of Cheonggukjang fermented with Bacillus subtilis. Food Sci. Biotechnol. 13: 572-575 (2004)
  8. Kang MJ, Kim JI, Kwon TW. Effect of cheonggukjang on blood glucose and lipid profile in neonatal streptozotocin-induced diabetic rats. Food Sci. Biotechnol. 12: 544-547 (2003)
  9. Kim W, Choi K, Kim Y, Park H, Choi J, Lee Y, Oh H, Kwon I, Lee S. Purification and characterization of fibrinolytic enzyme produced from Bacillus sp. strain CK-11-4 screened from chungkuk-jang. Appl. Environ. Microbiol. 62: 2482-2488 (1996)
  10. Lee CH, Yang EI, Song GS, Chai OH and Kim YS. Cheonggukjang mucilage stimulates immunohistochemical activities of gastrointestinal tract in rats. Food Sci. Biotechnol. 14: 813-817 (2005)
  11. Lee SK, Heo S, Bac DH, Choi KH. Medium optimization for fibrinolytic enzyme production by Bacillus subtilis KCK-7 isolated from Korean traditional chungkukjang. J. Appl. Microb. Biotechnol. 26: 226-231 (1998)
  12. Yoo JY. Present status of industries and research activities of Korean fermented soybean products. Microorg. Ind. 23: 13-30 (1997)
  13. Ishizuka S, Tanaka S, Xu H, Hara H. Fermentable dietary fiber potentiates the local ization of immune cells in the rat large intestinal crypts. Exp. Biol. Med. 229: 876-884 (2004) https://doi.org/10.1177/153537020422900903
  14. Vega-Lopez NA, Telemo E, Bailey M, Stevens K, Stokes CR. Immune cell distribution in the small intestine of the pig: immunohistological evidence for an organized compartmentalization in the lamina propria. Vet. Irnmunol. Immunopathol. 37: 49-60 (1993) https://doi.org/10.1016/0165-2427(93)90015-V
  15. Beagley KW, Husband AJ. Intraepithelial lymphocytes: origins, distribution, and function. Crit. Rev. Immunol. 18: 237-254 (1998) https://doi.org/10.1615/CritRevImmunol.v18.i3.40
  16. Blottiere HM, Buecher B, Galmiche JP, Cherbut C. Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation. Proc. Nutr. Soc. 62: 101-106 (2003)
  17. Moncada S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathophysiology and pharmacology. Phamacol. Rev. 43: 109-141 (1991)
  18. Konturek SK, Konturek PC. Role of nitric oxide in the digestive system. Digestion 56: 1-13 (1995)
  19. Sanders KM, Sean MW. Nitric oxide as a mediator of nonadrenergic noncholinergic neurotransmission. Am. J. Physiol. 262: G379-392 (1992)
  20. Nishizuka Y. Protein kinase C and lipid signaling for sustained cellular responses. FASEB J. 9: 484-496 (1995) https://doi.org/10.1096/fasebj.9.7.7737456
  21. Rickard KL, Gibson PR, Wilson NJ, Mariadason JM, Phillips WA. Short-chain fatty acids reduce expression of specific protein kinase C isoforms in human colonic epithelial cells. J. Cell Physiol. 182: 222-231 (2000) https://doi.org/10.1002/(SICI)1097-4652(200002)182:2<222::AID-JCP11>3.0.CO;2-B