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황산화 세균 Thiobacillus sp. UIW-6 변이주의 성장 최적화

Optimization of Mutant Strain of the Sulfur-Oxidizing Bacteria, Thiobacillus sp. UIW-6

  • 발행 : 2006.06.30

초록

황 화합물 악취를 제거하기 위하여 UV-B를 돌연변이원으로 처리한 후 공시균주 Thiobacillus sp. IW보다 성장속도가 빠른 변이주 UIW-6를 분리하여 최적성장 조건을 조사하였다. 변이주 UIW-6의 특성을 조사한 결과 최적조건은 pH 6.5, 배양온도는 $35^{\circ}C$이었으며, 회전속도는 200 rpm 이었다. 황 화합물인 sodium thiosulfate 농도가 50 mM에서 변이주 UIW-6는 6시간째에 공시균주 IW보다 성장정도가 2배 더 빨랐다. 변이주 UIW-6의 성장 최적 탄소원은 fructose와 sucrose였으며 질소원은 yeast extract> tryptone> peptone 순으로 이용하였다. 변이주 UIW-6는 공시균주로 사용한 IW 보다 성장이 빨라 황 화합물 악취 제거에 효과적으로 이용할 수 있는 유용한 변이주로 판단되어진다.

키워드

Thiobacillus sp. IW;UV-B;sodium thiosulfate;odor removal

참고문헌

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