Optimization of Mutant Strain of the Sulfur-Oxidizing Bacteria, Thiobacillus sp. UIW-6

황산화 세균 Thiobacillus sp. UIW-6 변이주의 성장 최적화

  • Published : 2006.06.30


To reducing offensive odor form compost UIW-6 mutant obtained by UV treatment from sulfur-oxidizing bacteria, Thiobacillus sp. IW. The UIW-6 mutant was found 1.6 times faster in growth than the parent strain on thiosulfate medium (TM) at 36 h after incubation. Initial pH, temperature and agitation for the optimum growth of UIW-6 were 6.5, $35^{\circ}C$ and 200 rpm, respectively. The UIW-6 mutant growth was two times higher than parent strain at 6 h culture in TM liquid medium containing 50 mM sodium thiosulfate. The UIW-6 mutant used fructose and sucrose as carbon sources and yeast extract> tryptone> peptone as nitrogen ones. It was found that the growth of UIW-6 was increased in addition of 0.2% yeast extract.


  1. American Public Health Association (1995) American Water Works Association and Water Environment Federation. Standard Methods for the Examination of Water and Wastewater, 19th ed., APHA, Washington, DC. USA
  2. Hong, S. S. and Lee, N. H. (1993) Growth of Spirulina platensis in effluents from wastewater treatment plant of pig farm, J. Microbial. Biotechnol. 3, 19-23
  3. Manios, T. (2004), The composting potential of different organic solid wastes: experience from the island of Crete, Environ. Int. 29, 1079-1089
  4. Cho, K. S., Hirai, M. and Shoda, M. (1992) Enhanced removability of odorous sulfur containing gases by mixed cultures of purified bacteria from peat biofilters, J. Ferment. and Bioeng. 73, 219-224
  5. Cha, J. M and Lee, I. W. (1995) Removal of hydrogen sulfide by immobilized Thiobacillus sp. IW. on polyvinylpyridine in a bubble column reactor, J. KSEE. 17, 145-155
  6. Cha, J. M., Park, Y. and Lee, I. W. (1994) Effects of cultivation condition on growth of hydrogen sulfidedegradating Thiobacillus sp. IW. isolated from waste coal mine water, Kor. J. Biotechnol. Bioeng. 9, 287-293
  7. Kwon, K. H., Cha, W. 5., Go, H. C, Lee, K. V.. Park, D. H. and Cha, J. M. (2004) Optimization of the sulfur-oxidizing bacteria, Thiobacillus novellus SRM, Kor. J. Biotechnol. Bioeng. 18, 443-447
  8. Wehner, J. and Homeck, G. (1995) Effects of vacuum UV and UVC radiation on dry E. coli plasmid pUC19. I. Inactivation, lacZ- mutation induction and strand breaks, J. Photochem. Photobiol. B: BioI. 28, 77-85
  9. Lee, S. K and Shoda, M (1989) Biological deodorization using activated carbon fabric as a carrier of microorganisms, J. Ferment. and Bioeng. 68, 437-442